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Calpain 9 (CAPN9) (NM_016452) Human Tagged ORF Clone
CAT＃: RC211502
CAPN9 (Myc-DDK-tagged)-Human calpain 9 (CAPN9), transcript variant 2
ORF Plasmid: tGFP
"NM_016452" in other vectors (4)
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CNY 4,944.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GC36; nCL-4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211502 representing NM_016452
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTACCTCTACCGGGCCCCAGGGCCTCAGGCACACCCGGTTCCCAAGGACGCCCGGATCACCCACT CCTCAGGCCAGAGCTTTGAGCAAATGAGGCAGGAGTGCCTGCAGAGAGGCACCCTGTTTGAGGATGCAGA CTTCCCAGCCAGCAATTCCTCCCTGTTCTACAGTGAGAGGCCGCAGATCCCCTTTGTGTGGAAACGACCA GGGGAAATCGTGAAAAACCCAGAATTCATTCTTGGAGGGGCCACCAGGACTGATATCTGCCAGGGAGAGC TGGGAGACTGCTGGCTATTAGCCGCCATCGCCTCCCTTACGCTTAATCAAAAAGCACTGGCCAGAGTCAT CCCCCAGGACCAAAGCTTTGGCCCTGGTTATGCCGGGATATTCCATTTCCAGTTCTGGCAGCACAGTGAG TGGCTGGACGTGGTGATCGATGACCGCCTGCCCACCTTCAGGGACCGCTTGGTTTTCCTCCACTCTGCCG ACCACAACGAGTTCTGGAGCGCCTTGCTGGAAAAAGCCTACGCCAAGCTAAATGGGAGCTATGAAGCTCT GAAGGGAGGCAGCGCCATCGAGGCCATGGAAGACTTCACTGGGGGTGTGGCAGAGACCTTCCAAACTAAA GAGGCCCCCGAGAACTTCTATGAGATTCTAGAGAAGGCTTTGAAGAGAGGCTCCCTGCTGGGCTGCTTCA TTGATACCAGAAGTGCTGCAGAATCTGAGGCCCGGACGCCGTTTGGTCTTATTAAGGGTCATGCCTACAG TGTAACGGGAATTGACCAGGTAAGCTTCCGAGGCCAGAGAATCGAGCTCATCCGAATCCGGAACCCTTGG GGCCAGGTTGAGTGGAACGGGTCGTGGAGCGACAGGATGGCATTTAAGGACTTCAAGGCCCACTTTGATA AAGTGGAGATCTGCAACCTCACTCCCGATGCCCTGGAGGAAGACGCGATCCACAAATGGGAGGTGACGGT CCATCAGGGAAGCTGGGTTCGCGGCTCCACGGCTGGGGGCTGCCGCAATTTCCTGGATACCTTTTGGACC AATCCACAAATAAAATTGTCTCTGACTGAGAAAGATGAGGGGCAGGAGGAGTGTAGTTTCCTTGTAGCCC TGATGCAGAAAGATAGAAGGAAACTCAAGAGATTTGGTGCCAATGTGCTGACAATCGGCTATGCCATTTA TGAGTGCCCTGACAAAGACGAACACCTGAACAAAGACTTCTTCAGATACCACGCTTCTCGGGCCAGAAGC AAGACGTTCATCAACCTGAGAGAAGTCTCCGACCGGTTCAAGCTGCCCCCTGGGGAGTACATCCTGATTC CCAGCACTTTTGAGCCCCACCAGGAAGCTGATTTCTGTCTGAGAATCTTTTCAGAGAAAAAAGCCATTAC CCGGGATATGGATGGAAATGTAGACATTGACCTTCCTGAGCCTCCAAAGCCAACTCCACCTGACCAGGAG ACAGAGGAGGAGCAGCGGTTTCGGGCTCTGTTTGAACAAGTCGCTGGTGAGGACATGGAGGTGACAGCAG AGGAACTTGAGTATGTTTTAAATGCTGTGCTGCAAAAGAAAAAGGACATCAAATTCAAGAAGCTAAGCCT GATCTCCTGTAAAAACATCATTTCCCTGATGGACACCAGCGGCAATGGGAAGCTGGAGTTTGATGAATTC AAAGTGTTCTGGGACAAGCTGAAGCAGTGGATTAACCTTTTCCTTCGGTTTGATGCTGACAAGTCCGGCA CCATGTCTACCTATGAACTACGGACTGCACTGAAAGCTGCAGGCTTTCAGCTGAGCAGCCACCTCCTGCA GCTGATTGTGCTCAGGTATGCGGATGAGGAGCTCCAGCTGGACTTCGATGACTTCCTCAACTGCCTGGTC CGGCTGGAGAATGCGAGCCGGGTGTTCCAGGCTCTCAGTACAAAGAACAAGGAGTTCATTCATCTCAATA TAAATGAGTTCATCCATTTGACAATGAACATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211502 representing NM_016452
Red=Cloning site Green=Tags(s) MPYLYRAPGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRP GEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSE WLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQTK EAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPW GQVEWNGSWSDRMAFKDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWT NPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDKDEHLNKDFFRYHASRARS KTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDIDLPEPPKPTPPDQE TEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEF KVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFLNCLV RLENASRVFQALSTKNKEFIHLNINEFIHLTMNI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016452 |
| ORF Size | 1992 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016452.2, NP_057536.1 |
| RefSeq Size | 2484 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 10753 |
| UniProt ID | O14815 |
| Protein Families | Druggable Genome, Protease |
| MW | 76 kDa |
| Gene Summary | Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211502L3 | Lenti-ORF clone of CAPN9 (Myc-DDK-tagged)-Human calpain 9 (CAPN9), transcript variant 2 |
CNY 6,460.00 |
|
| RC211502L4 | Lenti-ORF clone of CAPN9 (mGFP-tagged)-Human calpain 9 (CAPN9), transcript variant 2 |
CNY 6,460.00 |
|
| RG211502 | CAPN9 (tGFP-tagged) - Human calpain 9 (CAPN9), transcript variant 2 |
CNY 5,040.00 |
|
| SC304422 | CAPN9 (untagged)-Human calpain 9 (CAPN9), transcript variant 2 |
CNY 10,640.00 |
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