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PHKA1 (NM_002637) Human Tagged ORF Clone
CAT＃: RC211553
PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1
ORF Plasmid: tGFP
"NM_002637" in other vectors (4)
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CNY 3,744.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PHKA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211553 representing NM_002637
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTGT GCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAA TGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGAT AAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCA GACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAGTACAA CACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTG TACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGATGAAGTCAATT TCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTCGGGATATGGGAACGTGG AGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGGCAGCCCTGGAA GCATTAGATGAACTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATG AAGTACAGCACTGCCAGTCTATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAGGTTGATGC TAGTCTACTCTCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAA CAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGATATAAAACTC CTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGTGA ATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTCTTCAGTGGCAATGCAGAACAGGTTCAAGAA TATAAAGAGGCTCTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACA GTGTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCCATGGGGAA ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCT GGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTTCAAGTCTCCA TTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATTTACGTGGAGACCATTGCTGA GGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGCCACATTTATTCCAGCCTAGGATGCAACAAT AGAATGAAACTCAGTGGACGACCCTACAGACACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTC GGAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACAA GATGATAGTGGAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCC ACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCAAGTATCCTGG CAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAAACAGGTAAATTGTCAGAGTT TTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA GATTATGATGACAACTATGATTACCTGGAATCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATG CTCGCTGTGGTGATGAAGTTGCTCGTTATTTAGATCACCTTTTGGCGCACACTGCTCCCCATCCTAAACT AGCCCCTACCTCACAGAAGGGAGGGCTAGATCGGTTCCAAGCTGCTGTGCAAACAACCTGCGACTTAATG TCCTTGGTGACCAAGGCCAAGGAACTGCATGTACAGAATGTTCACATGTATCTTCCTACGAAGTTATTTC AGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACCTCATCCCCGACAGGAGAACCAGGTTCCCTCTGT TCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGGGAGGTGGACTTTAAAGCACTGGTTTTACAGTTG AAGGAGACCTCAAGCTTACAGGAACAAGCTGATATCCTCTATATGCTGTATACTATGAAAGGACCTGACT GGAACACTGAATTGTATAATGAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTATGGCAAAGT GGGAGAAATTCGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCACTT GATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTGACAGTAGGACTTCCTCCAGAACCTCGAG AAAAGACTATCTCTGCACCTCTGCCCTATGAGGCGCTCACTCAGCTGATAGATGAAGCCAGTGAAGGGGA TATGAGCATTTCAATCCTTACACAGGAAATAATGGTATATCTAGCCATGTATATGCGAACCCAGCCTGGC CTCTTTGCTGAAATGTTTCGACTTCGAATTGGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACT CCCTTCGATGCTCAGCTGAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATGAAGAATCT CCTGCATCACATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTC AGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCAACCAAAACAGAACGAACTGGGATCATGC AGTTAAAAAGTGAGATAAAGCAGTCACCTGGAACCTCTATGACTCCAAGTAGTGGGTCCTTTCCTAGTGC ATATGATCAGCAGTCATCTAAAGATAGTCGTCAAGGTCAATGGCAACGCCGAAGAAGGCTGGATGGGGCA CTGAATAGAGTTCCAGTTGGATTTTATCAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTG TTGAAGGGTTTGTCCTTCCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTCTCTGTTCA TGTGGAGTCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTGGTTGAAGCCATCCTTGTC CTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGCATCATTGCTGTGGAAAAAATAGTGCATA TTGCCAATGACTTGTTCCTTCAAGAACAGAAAACCCTTGGCGCAGATGATACCATGTTGGCAAAGGATCC CGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCACCCAGTGGCAGGTTTGGCACCATGACCTACCTC TCCAAGGCAGCCGCCACCTACGTGCAGGAGTTCCTGCCCCACAGCATCTGTGCCATGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211553 representing NM_002637
Red=Cloning site Green=Tags(s) MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDED KAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSV YLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALE ALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTK QEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQE YKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNN RMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQP TITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSE DYDDNYDYLESGNWMNDYDSTSHARCGDEVARYLDHLLAHTAPHPKLAPTSQKGGLDRFQAAVQTTCDLM SLVTKAKELHVQNVHMYLPTKLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSGEVDFKALVLQL KETSSLQEQADILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEAL DEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPG LFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNV SPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQRRRRLDGA LNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILV LTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYL SKAAATYVQEFLPHSICAMQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002637 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 4215 bp |
| RefSeq ORF | 3672 bp |
| Locus ID | 5255 |
| UniProt ID | P46020 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Insulin signaling pathway |
| MW | 135.81 kDa |
| Gene Summary | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211553L3 | Lenti-ORF clone of PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 14,730.00 |
|
| RC211553L4 | Lenti-ORF clone of PHKA1 (mGFP-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 14,730.00 |
|
| RG211553 | PHKA1 (tGFP-tagged) - Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 5,344.00 |
|
| SC118481 | PHKA1 (untagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 8,520.00 |
