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PKC mu (PRKD1) (NM_002742) Human Tagged ORF Clone
CAT＃: RC211676
PRKD1 (Myc-DDK-tagged)-Human protein kinase D1 (PRKD1)
ORF Plasmid: tGFP
"NM_002742" in other vectors (7)
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CNY 6,680.00
CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CHDED; PKC-MU; PKCM; PKD; PRKCM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211676 representing NM_002742
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCCCCTCCGGTCCTGCGGCCGCCCAGTCCGCTGCTGCCCGTGGCGGCGGCAGCTGCCGCAGCGG CCGCCGCACTGGTCCCAGGGTCCGGGCCCGGGCCCGCGCCGTTCTTGGCTCCTGTCGCGGCCCCGGTCGG GGGCATCTCGTTCCATCTGCAGATCGGCCTGAGCCGTGAGCCGGTGCTGCTGCTGCAGGACTCGTCCGGG GACTACAGCCTGGCGCACGTCCGCGAGATGGCTTGCTCCATTGTCGACCAGAAGTTCCCTGAATGTGGTT TCTACGGAATGTATGATAAGATCCTGCTTTTTCGCCATGACCCTACCTCTGAAAACATCCTTCAGCTGGT GAAAGCGGCCAGTGATATCCAGGAAGGCGATCTTATTGAAGTGGTCTTGTCAGCTTCCGCCACCTTTGAA GACTTTCAGATTCGTCCCCACGCTCTCTTTGTTCATTCATACAGAGCTCCAGCTTTCTGTGATCACTGTG GAGAAATGCTGTGGGGGCTGGTACGTCAAGGTCTTAAATGTGAAGGGTGTGGTCTGAATTACCATAAGAG ATGTGCATTTAAAATACCCAACAATTGCAGCGGTGTGAGGCGGAGAAGGCTCTCAAACGTTTCCCTCACT GGGGTCAGCACCATCCGCACATCATCTGCTGAACTCTCTACAAGTGCCCCTGATGAGCCCCTTCTGCAAA AATCACCATCAGAGTCGTTTATTGGTCGAGAGAAGAGGTCAAATTCTCAATCATACATTGGACGACCAAT TCACCTTGACAAGATTTTGATGTCTAAAGTTAAAGTGCCGCACACATTTGTCATCCACTCCTACACCCGG CCCACAGTGTGCCAGTACTGCAAGAAGCTTCTGAAGGGGCTTTTCAGGCAGGGCTTGCAGTGCAAAGATT GCAGATTCAACTGCCATAAACGTTGTGCACCGAAAGTACCAAACAACTGCCTTGGCGAAGTGACCATTAA TGGAGATTTGCTTAGCCCTGGGGCAGAGTCTGATGTGGTCATGGAAGAAGGGAGTGATGACAATGATAGT GAAAGGAACAGTGGGCTCATGGATGATATGGAAGAAGCAATGGTCCAAGATGCAGAGATGGCAATGGCAG AGTGCCAGAACGACAGTGGCGAGATGCAAGATCCAGACCCAGACCACGAGGACGCCAACAGAACCATCAG TCCATCAACAAGCAACAATATCCCACTCATGAGGGTAGTGCAGTCTGTCAAACACACGAAGAGGAAAAGC AGCACAGTCATGAAAGAAGGATGGATGGTCCACTACACCAGCAAGGACACGCTGCGGAAACGGCACTATT GGAGATTGGATAGCAAATGTATTACCCTCTTTCAGAATGACACAGGAAGCAGGTACTACAAGGAAATTCC TTTATCTGAAATTTTGTCTCTGGAACCAGTAAAAACTTCAGCTTTAATTCCTAATGGGGCCAATCCTCAT TGTTTCGAAATCACTACGGCAAATGTAGTGTATTATGTGGGAGAAAATGTGGTCAATCCTTCCAGCCCAT CACCAAATAACAGTGTTCTCACCAGTGGCGTTGGTGCAGATGTGGCCAGGATGTGGGAGATAGCCATCCA GCATGCCCTTATGCCCGTCATTCCCAAGGGCTCCTCCGTGGGTACAGGAACCAACTTGCACAGAGATATC TCTGTGAGTATTTCAGTATCAAATTGCCAGATTCAAGAAAATGTGGACATCAGCACAGTATATCAGATTT TTCCTGATGAAGTACTGGGTTCTGGACAGTTTGGAATTGTTTATGGAGGAAAACATCGTAAAACAGGAAG AGATGTAGCTATTAAAATCATTGACAAATTACGATTTCCAACAAAACAAGAAAGCCAGCTTCGTAATGAG GTTGCAATTCTACAGAACCTTCATCACCCTGGTGTTGTAAATTTGGAGTGTATGTTTGAGACGCCTGAAA GAGTGTTTGTTGTTATGGAAAAACTCCATGGAGACATGCTGGAAATGATCTTGTCAAGTGAAAAGGGCAG GTTGCCAGAGCACATAACGAAGTTTTTAATTACTCAGATACTCGTGGCTTTGCGGCACCTTCATTTTAAA AATATCGTTCACTGTGACCTCAAACCAGAAAATGTGTTGCTAGCCTCAGCTGATCCTTTTCCTCAGGTGA AACTTTGTGATTTTGGTTTTGCCCGGATCATCGGAGAGAAGTCTTTCCGGAGGTCAGTGGTGGGTACCCC CGCTTACCTGGCTCCTGAGGTCCTAAGGAACAAGGGCTACAATCGCTCTCTAGACATGTGGTCTGTTGGG GTCATCATCTATGTAAGCCTAAGCGGCACATTCCCATTTAATGAAGATGAAGACATACACGACCAAATTC AGAATGCAGCTTTCATGTATCCACCAAATCCCTGGAAGGAAATATCTCATGAAGCCATTGATCTTATCAA CAATTTGCTGCAAGTAAAAATGAGAAAGCGCTACAGTGTGGATAAGACCTTGAGCCACCCTTGGCTACAG GACTATCAGACCTGGTTAGATTTGCGAGAGCTGGAATGCAAAATCGGGGAGCGCTACATCACCCATGAAA GTGATGACCTGAGGTGGGAGAAGTATGCAGGCGAGCAGGGGCTGCAGTACCCCACACACCTGATCAATCC AAGTGCTAGCCACAGTGACACTCCTGAGACTGAAGAAACAGAAATGAAAGCCCTCGGTGAGCGTGTCAGC ATCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211676 representing NM_002742
Red=Cloning site Green=Tags(s) MSAPPVLRPPSPLLPVAAAAAAAAAALVPGSGPGPAPFLAPVAAPVGGISFHLQIGLSREPVLLLQDSSG DYSLAHVREMACSIVDQKFPECGFYGMYDKILLFRHDPTSENILQLVKAASDIQEGDLIEVVLSASATFE DFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLT GVSTIRTSSAELSTSAPDEPLLQKSPSESFIGREKRSNSQSYIGRPIHLDKILMSKVKVPHTFVIHSYTR PTVCQYCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGDLLSPGAESDVVMEEGSDDNDS ERNSGLMDDMEEAMVQDAEMAMAECQNDSGEMQDPDPDHEDANRTISPSTSNNIPLMRVVQSVKHTKRKS STVMKEGWMVHYTSKDTLRKRHYWRLDSKCITLFQNDTGSRYYKEIPLSEILSLEPVKTSALIPNGANPH CFEITTANVVYYVGENVVNPSSPSPNNSVLTSGVGADVARMWEIAIQHALMPVIPKGSSVGTGTNLHRDI SVSISVSNCQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNE VAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFK NIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVG VIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTLSHPWLQ DYQTWLDLRELECKIGERYITHESDDLRWEKYAGEQGLQYPTHLINPSASHSDTPETEETEMKALGERVS IL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002742 |
| ORF Size | 2736 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002742.3 |
| RefSeq Size | 3742 bp |
| RefSeq ORF | 2739 bp |
| Locus ID | 5587 |
| UniProt ID | Q15139 |
| Domains | pkinase, TyrKc, PH, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 101.5 kDa |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211676L1 | Lenti ORF clone of Human protein kinase D1 (PRKD1), Myc-DDK-tagged |
CNY 9,080.00 |
|
| RC211676L2 | Lenti ORF clone of Human protein kinase D1 (PRKD1), mGFP tagged |
CNY 11,500.00 |
|
| RC211676L3 | Lenti ORF clone of Human protein kinase D1 (PRKD1), Myc-DDK-tagged |
CNY 9,080.00 |
|
| RC211676L4 | Lenti ORF clone of Human protein kinase D1 (PRKD1), mGFP tagged |
CNY 9,080.00 |
|
| RG211676 | PRKD1 (tGFP-tagged) - Human protein kinase D1 (PRKD1) |
CNY 8,280.00 |
|
| SC118457 | PRKD1 (untagged)-Human protein kinase D1 (PRKD1) |
CNY 6,688.00 |
|
| SC323698 | PRKD1 (untagged)-Kinase deficient mutant (K612M) of Human protein kinase D1 (PRKD1) |
CNY 6,688.00 |
