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VLDL Receptor (VLDLR) (NM_003383) Human Tagged ORF Clone
CAT＃: RC211796
VLDLR (Myc-DDK-tagged)-Human very low density lipoprotein receptor (VLDLR), transcript variant 1
ORF Plasmid: tGFP
"NM_003383" in other vectors (6)
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CNY 9,592.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAMRQ1; CARMQ1; CHRMQ1; VLDL-R; VLDLRCH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211796 representing NM_003383
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACGTCCGCGCTCTGGGCGCTCTGGCTGCTGCTCGCGCTGTGCTGGGCGCCCCGGGAGAGCGGCG CCACCGGAACCGGGAGAAAAGCCAAATGTGAACCCTCCCAATTCCAGTGCACAAATGGTCGCTGTATTAC GCTGTTGTGGAAATGTGATGGGGATGAAGACTGTGTTGACGGCAGTGATGAAAAGAACTGTGTAAAGAAG ACGTGTGCTGAATCTGACTTCGTGTGCAACAATGGCCAGTGTGTTCCCAGCCGATGGAAGTGTGATGGAG ATCCTGACTGCGAAGATGGTTCAGATGAAAGCCCAGAACAGTGCCATATGAGAACATGCCGCATACATGA AATCAGCTGTGGCGCCCATTCTACTCAGTGTATCCCAGTGTCCTGGAGATGTGATGGTGAAAATGATTGT GACAGTGGAGAAGATGAAGAAAACTGTGGCAATATAACATGTAGTCCCGACGAGTTCACCTGCTCCAGTG GCCGCTGCATCTCCAGGAACTTTGTATGCAATGGCCAGGATGACTGCAGCGATGGCAGTGATGAGCTGGA CTGTGCCCCGCCAACCTGTGGCGCCCATGAGTTCCAGTGCAGCACCTCCTCCTGCATCCCCATCAGCTGG GTATGCGACGATGATGCAGACTGCTCCGACCAATCTGATGAGTCCCTGGAGCAGTGTGGCCGTCAGCCAG TCATACACACCAAGTGTCCAGCCAGCGAAATCCAGTGCGGCTCTGGCGAGTGCATCCATAAGAAGTGGCG ATGTGATGGGGACCCTGACTGCAAGGATGGCAGTGATGAGGTCAACTGTCCCTCTCGAACTTGCCGACCT GACCAATTTGAATGTGAGGATGGCAGCTGCATCCATGGCAGCAGGCAGTGTAATGGTATCCGAGACTGTG TCGATGGTTCCGATGAAGTCAACTGCAAAAATGTCAATCAGTGCTTGGGCCCTGGAAAATTCAAGTGCAG AAGTGGAGAATGCATAGATATCAGCAAAGTATGTAACCAGGAGCAGGACTGCAGGGACTGGAGTGATGAG CCCCTGAAAGAGTGTCATATAAACGAATGCTTGGTAAATAATGGTGGATGTTCTCATATCTGCAAAGACC TAGTTATAGGCTACGAGTGTGACTGTGCAGCTGGGTTTGAACTGATAGATAGGAAAACCTGTGGAGATAT TGATGAATGCCAAAATCCAGGAATCTGCAGTCAAATTTGTATCAACTTAAAAGGCGGTTACAAGTGTGAA TGTAGTCGTGGCTATCAAATGGATCTTGCTACTGGCGTGTGCAAGGCAGTAGGCAAAGAGCCAAGTCTGA TCTTCACTAATCGAAGAGACATCAGGAAGATTGGCTTAGAGAGGAAAGAATATATCCAACTAGTTGAACA GCTAAGAAACACTGTGGCTCTCGATGCTGACATTGCTGCCCAGAAACTATTCTGGGCCGATCTAAGCCAA AAGGCTATCTTCAGTGCCTCAATTGATGACAAGGTTGGTAGACATGTTAAAATGATCGACAATGTCTATA ATCCTGCAGCCATTGCTGTTGATTGGGTGTACAAGACCATCTACTGGACTGATGCGGCTTCTAAGACTAT TTCAGTAGCTACCCTAGATGGAACCAAGAGGAAGTTCCTGTTTAACTCTGACTTGCGAGAGCCTGCCTCC ATAGCTGTGGACCCACTGTCTGGCTTTGTTTACTGGTCAGACTGGGGTGAACCAGCTAAAATAGAAAAAG CAGGAATGAATGGATTCGATAGACGTCCACTGGTGACAGCGGATATCCAGTGGCCTAACGGAATTACACT TGACCTTATAAAAAGTCGCCTCTATTGGCTTGATTCTAAGTTGCACATGTTATCCAGCGTGGACTTGAAT GGCCAAGATCGTAGGATAGTACTAAAGTCTCTGGAGTTCCTAGCTCATCCTCTTGCACTAACAATATTTG AGGATCGTGTCTACTGGATAGATGGGGAAAATGAAGCAGTCTATGGTGCCAATAAATTCACTGGATCAGA GCTAGCCACTCTAGTCAACAACCTGAATGATGCCCAAGACATCATTGTCTATCATGAACTTGTACAGCCA TCAGGTAAAAATTGGTGTGAAGAAGACATGGAGAATGGAGGATGTGAATACCTATGCCTGCCAGCACCAC AGATTAATGATCACTCTCCAAAATATACCTGTTCCTGTCCCAGTGGGTACAATGTAGAGGAAAATGGCCG AGACTGTCAAAGTACTGCAACTACTGTGACTTACAGTGAGACAAAAGATACGAACACAACAGAAATTTCA GCAACTAGTGGACTAGTTCCTGGAGGGATCAATGTGACCACAGCAGTATCAGAGGTCAGTGTTCCCCCAA AAGGGACTTCTGCCGCATGGGCCATTCTTCCTCTCTTGCTCTTAGTGATGGCAGCAGTAGGTGGCTACTT GATGTGGCGGAATTGGCAACACAAGAACATGAAAAGCATGAACTTTGACAATCCTGTGTACTTGAAAACC ACTGAAGAGGACCTCTCCATAGACATTGGTAGACACAGTGCTTCTGTTGGACACACGTACCCAGCAATAT CAGTTGTAAGCACAGATGATGATCTAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211796 representing NM_003383
Red=Cloning site Green=Tags(s) MGTSALWALWLLLALCWAPRESGATGTGRKAKCEPSQFQCTNGRCITLLWKCDGDEDCVDGSDEKNCVKK TCAESDFVCNNGQCVPSRWKCDGDPDCEDGSDESPEQCHMRTCRIHEISCGAHSTQCIPVSWRCDGENDC DSGEDEENCGNITCSPDEFTCSSGRCISRNFVCNGQDDCSDGSDELDCAPPTCGAHEFQCSTSSCIPISW VCDDDADCSDQSDESLEQCGRQPVIHTKCPASEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRP DQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDISKVCNQEQDCRDWSDE PLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCE CSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQ KAIFSASIDDKVGRHVKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGTKRKFLFNSDLREPAS IAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTADIQWPNGITLDLIKSRLYWLDSKLHMLSSVDLN GQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQP SGKNWCEEDMENGGCEYLCLPAPQINDHSPKYTCSCPSGYNVEENGRDCQSTATTVTYSETKDTNTTEIS ATSGLVPGGINVTTAVSEVSVPPKGTSAAWAILPLLLLVMAAVGGYLMWRNWQHKNMKSMNFDNPVYLKT TEEDLSIDIGRHSASVGHTYPAISVVSTDDDLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003383 |
| ORF Size | 2619 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003383.5 |
| RefSeq Size | 3646 bp |
| RefSeq ORF | 2622 bp |
| Locus ID | 7436 |
| UniProt ID | P98155 |
| Domains | ldl_recept_b, EGF_CA, ldl_recept_a, EGF |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 96.1 kDa |
| Gene Summary | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Roles of the low density lipoprotein receptor and related receptors in inhibition of lipoprotein(a) internalization by proprotein convertase subtilisin/kexin type 9
,null,
PLoS ONE
,PubMed ID 28750079
[VLDL Receptor]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211796L1 | Lenti ORF clone of Human very low density lipoprotein receptor (VLDLR), transcript variant 1, Myc-DDK-tagged |
CNY 11,992.00 |
|
| RC211796L2 | Lenti ORF clone of Human very low density lipoprotein receptor (VLDLR), transcript variant 1, mGFP tagged |
CNY 11,992.00 |
|
| RC211796L3 | Lenti ORF clone of Human very low density lipoprotein receptor (VLDLR), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211796L4 | Lenti ORF clone of Human very low density lipoprotein receptor (VLDLR), transcript variant 1, mGFP tagged |
CNY 11,992.00 |
|
| RG211796 | VLDLR (tGFP-tagged) - Human very low density lipoprotein receptor (VLDLR), transcript variant 1 |
CNY 11,192.00 |
|
| SC117993 | VLDLR (untagged)-Human very low density lipoprotein receptor (VLDLR), transcript variant 1 |
CNY 9,600.00 |
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