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FMRFamide (NPFF) (NM_003717) Human Tagged ORF Clone
CAT＃: RC211960
- TrueORF®
NPFF (Myc-DDK-tagged)-Human neuropeptide FF-amide peptide precursor (NPFF)
ORF Plasmid: tGFP
"NM_003717" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FMRFAL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211960 representing NM_003717
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCTAGGCAGGCTGCTGCACTGCTGGTGCTGCTGCTGTTAATAGACGGGGGCTGTGCTGAAGGGC CAGGAGGCCAGCAGGAAGACCAGCTCTCCGCGGAGGAAGACAGCGAACCCCTCCCACCACAGGATGCCCA GACCTCTGGGTCACTGTTGCACTACCTGCTCCAGGCAATGGAGAGACCTGGCCGGAGCCAAGCCTTCCTG TTTCAGCCCCAGAGGTTTGGCAGAAATACCCAGGGATCCTGGAGGAATGAATGGCTGAGTCCCCGGGCTG GAGAGGGGCTGAATTCCCAGTTCTGGAGCCTGGCTGCCCCTCAACGCTTTGGGAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211960 representing NM_003717
Red=Cloning site Green=Tags(s) MDSRQAAALLVLLLLIDGGCAEGPGGQQEDQLSAEEDSEPLPPQDAQTSGSLLHYLLQAMERPGRSQAFL FQPQRFGRNTQGSWRNEWLSPRAGEGLNSQFWSLAAPQRFGKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003717 |
| ORF Size | 339 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003717.4 |
| RefSeq Size | 592 bp |
| RefSeq ORF | 342 bp |
| Locus ID | 8620 |
| UniProt ID | O15130 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 12.9 kDa |
| Gene Summary | This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211960L3 | Lenti-ORF clone of NPFF (Myc-DDK-tagged)-Human neuropeptide FF-amide peptide precursor (NPFF) |
CNY 5,890.00 |
|
| RC211960L4 | Lenti-ORF clone of NPFF (mGFP-tagged)-Human neuropeptide FF-amide peptide precursor (NPFF) |
CNY 5,890.00 |
|
| RG211960 | NPFF (tGFP-tagged) - Human neuropeptide FF-amide peptide precursor (NPFF) |
CNY 2,800.00 |
|
| SC117789 | NPFF (untagged)-Human neuropeptide FF-amide peptide precursor (NPFF) |
CNY 1,200.00 |
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