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C17orf85 (NCBP3) (NM_018553) Human Tagged ORF Clone
CAT＃: RC212022
C17orf85 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 85 (C17orf85), transcript variant 2
ORF Plasmid: tGFP
"NM_018553" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ELG; HSA277841 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212022 representing NM_018553
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATATGGGAATCCAAATTATGGAGGCATGAAAGGAATTCTTAGCAATTCATGGAAGCGAAGATATC ATTCCCGTCGTATTCAGCGGGACGTGATCAAGAAGAGAGCCCTGATTGGGGATGACGTTGGCTTGACGTC GTATAAACATCGACATTCTGGGCTAGTGAATGTTCCCGAGGAACCCATTGAAGAGGAGGAAGAGGAGGAG GAGGAGGAAGAGGAAGAGGAAGAAGAAGACCAGGACATGGATGCAGATGACAGAGTGGTGGTAGAGTACC ACGAGGAGCTCCCGGCTCTCAAGCAGCCCCGGGAGCGGAGCGCGTCTAGACGATCCAGTGCCAGCAGCTC AGACTCAGATGAAATGGACTATGATCTAGAACTGAAAATGATTTCCACGCCTTCACCAAAGAAAAGCATG AAAATGACTATGTATGCTGACGAAGTGGAATCTCAGTTGAAAAATATTAGGAACTCCATGAGGGCAGATA GTGTATCTTCAAGCAATATCAAAAACCGAATTGGTAACAAATTACCACCTGAGAAATTTGCAGATGTCCG ACATCTATTAGATGAGAAACGTCAGCACTCCCGTCCACGGCCACCAGTCAGCAGTACTAAATCAGATATA CGCCAGCGGTTAGGAAAAAGACCACATTCTCCGGAAAAGGCTTTTAGTAGTAACCCCGTCGTTCGGAGAG AGCCCTCTTCTGATGTGCATAGTAGGCTAGGTGTTCCCAGGCAGGATAGTAAAGGCCTCTACGCCGATAC TCGGGAGAAGAAATCAGGTAATTTATGGACTCGCCTAGGATCTGCACCCAAGACCAAAGAAAAGAATACG AAGAAAGTGGATCACAGGGCGCCTGGCGCTGAGGAAGACGACTCTGAGCTGCAAAGGGCATGGGGGGCTC TGATTAAGGAGAAAGAGCAGTCTCGCCAAAAGAAGAGCCGGTTAGATAACTTACCATCTCTCCAGATTGA AGTTAGTCGGGAAAGCAGCTCTGGTTCAGAGGCAGAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212022 representing NM_018553
Red=Cloning site Green=Tags(s) MKYGNPNYGGMKGILSNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEE EEEEEEEEEDQDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSM KMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPRPPVSSTKSDI RQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREKKSGNLWTRLGSAPKTKEKNT KKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDNLPSLQIEVSRESSSGSEAES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018553 |
| ORF Size | 1020 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018553.3 |
| RefSeq Size | 2709 bp |
| RefSeq ORF | 1022 bp |
| Locus ID | 55421 |
| MW | 38.7 kDa |
| Gene Summary | Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212022L3 | Lenti ORF clone of Human chromosome 17 open reading frame 85 (C17orf85), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212022L4 | Lenti ORF clone of Human chromosome 17 open reading frame 85 (C17orf85), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212022 | C17orf85 (tGFP-tagged) - Human chromosome 17 open reading frame 85 (C17orf85), transcript variant 2 |
CNY 4,370.00 |
|
| SC127383 | C17orf85 (untagged)-Human chromosome 17 open reading frame 85 (C17orf85), transcript variant 2 |
CNY 3,656.00 |
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