SIRT4 (NM_012240) Human Tagged ORF Clone

Name: SIRT4 (NM_012240) Human Tagged ORF Clone
SKU: RC212226
Price: 2400.00

CAT＃: RC212226

SIRT4 (Myc-DDK-tagged)-Human sirtuin 4 (SIRT4)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_012240" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00

货期*

现货

规格

Cited in 6 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA381511

SIRT4 Rabbit polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SIR2L4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC212226 representing NM_012240 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGAGCTTTGCGTTGACTTTCAGGTCAGCAAAAGGCCGTTGGATCGCAAACCCCAGCCAGCCGT GCTCGAAAGCCTCCATTGGGTTATTTGTGCCAGCAAGTCCTCCTCTGGACCCTGAGAAGGTCAAAGAGTT ACAGCGCTTCATCACCCTTTCCAAGAGACTCCTTGTGATGACTGGGGCAGGAATCTCCACCGAATCGGGG ATACCAGACTACAGGTCAGAAAAAGTGGGGCTTTATGCCCGCACTGACCGCAGGCCCATCCAGCATGGTG ATTTTGTCCGGAGTGCCCCAATCCGCCAGCGGTACTGGGCGAGAAACTTCGTAGGCTGGCCTCAATTCTC CTCCCACCAGCCTAACCCTGCACACTGGGCTTTGAGCACCTGGGAGAAACTCGGAAAGCTGTACTGGTTG GTGACCCAAAATGTGGATGCTTTGCACACCAAGGCGGGGAGTCGGCGCCTGACAGAGCTCCACGGATGCA TGGACAGGGTCCTGTGCTTGGATTGTGGGGAACAGACTCCCCGGGGGGTGCTGCAAGAGCGTTTCCAAGT CCTGAACCCCACCTGGAGTGCTGAGGCCCATGGCCTGGCTCCTGATGGTGACGTCTTTCTCTCAGAGGAG CAAGTCCGGAGCTTTCAGGTCCCAACCTGCGTTCAATGTGGAGGCCATCTGAAACCAGATGTCGTTTTCT TCGGGGACACAGTGAACCCTGACAAGGTTGATTTTGTGCACAAGCGTGTAAAAGAAGCCGACTCCCTCTT GGTGGTGGGATCATCCTTGCAGGTATACTCTGGTTACAGGTTTATCCTCACTGCCTGGGAGAAGAAGCTC CCGATTGCAATACTGAACATTGGGCCCACACGGTCGGATGACTTGGCGTGTCTGAAACTGAATTCTCGTT GTGGAGAGTTGCTGCCTTTGATAGACCCATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC212226 representing NM_012240 Red=Cloning site Green=Tags(s) MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASPPLDPEKVKELQRFITLSKRLLVMTGAGISTESG IPDYRSEKVGLYARTDRRPIQHGDFVRSAPIRQRYWARNFVGWPQFSSHQPNPAHWALSTWEKLGKLYWL VTQNVDALHTKAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEE QVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKL PIAILNIGPTRSDDLACLKLNSRCGELLPLIDPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_012240
ORF Size	942 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012240.2
RefSeq Size	1174 bp
RefSeq ORF	945 bp
Locus ID	23409
UniProt ID	Q9Y6E7
Domains	SIR2
Protein Families	Druggable Genome, Transcription Factors
MW	35 kDa
Gene Summary	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (6)

The use of this cDNA Clones has been cited in the following citations:
SIRT4 prevents excitotoxicity via modulating glutamate metabolism in glioma cells ,Yalçın, GD;Colak, M;, Hum Exp Toxicol ,PubMed ID 32081049 [SIRT4]
SIRT4 enhances the sensitivity of ER-positive breast cancer to tamoxifen by inhibiting the IL-6/STAT3 signal pathway ,Xing, J;Li, J;Fu, L;Gai, J;Guan, J;Li, Q;, Cancer Med ,PubMed ID 31573734 [SIRT4]
SIRT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion ,Sun, H;Huang, D;Liu, G;Jian, F;Zhu, J;Zhang, L;, Onco Targets Ther ,PubMed ID 30022839 [SIRT4]
SIRT4 interacts with OPA1 and regulates mitochondrial quality control and mitophagy ,Lang, A;Anand, R;Altinoluk-Hambüchen, S;Ezzahoini, H;Stefanski, A;Iram, A;Bergmann, L;Urbach, J;Böhler, P;Hänsel, J;Franke, M;Stühler, K;Krutmann, J;Scheller, J;Stork, B;Reichert, AS;Piekorz, RP;, Aging (Albany NY)2017 ,PubMed ID 29081403 [SIRT4]
SIRT4 inhibits malignancy progression of NSCLCs, through mitochondrial dynamics mediated by the ERK-Drp1 pathway ,Fu, L;Dong, Q;He, J;Wang, X;Xing, J;Wang, E;Qiu, X;Li, Q;, Oncogene ,PubMed ID 27941873 [SIRT4]
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activity ,null, The Journal of Cell Biology ,PubMed ID 25023514 [SIRT4]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC212226L1	Lenti ORF clone of Human sirtuin 4 (SIRT4), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC212226L2	Lenti ORF clone of Human sirtuin 4 (SIRT4), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC212226L3	Lenti ORF clone of Human sirtuin 4 (SIRT4), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC212226L4	Lenti ORF clone of Human sirtuin 4 (SIRT4), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RG212226	SIRT4 (tGFP-tagged) - Human sirtuin 4 (SIRT4)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,000.00 CNY 4,370.00
SC310543	SIRT4 (untagged)-Human sirtuin 4 (SIRT4)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 6,270.00