TBL1Y (NM_134259) Human Tagged ORF Clone

CAT#: RC212240

TBL1Y (Myc-DDK-tagged)-Human transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_134259" in other vectors (6)

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CNY 5,832.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-TBL1Y Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DFNY2; TBL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212240 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCATAACCAGTGACGAGGTGAACTTTCTGGTTTATCGGTATCTCCAGGAGTCAGGTTTTTCTCACT
CGGCTTTCACGTTTGGGATCGAGAGCCACATCAGCCAGTCCAACATCAATGGGACACTAGTGCCACCGTC
TGCCCTCATCTCCATTCTCCAGAAGGGACTGCAGTATGTAGAGGCTGAGATAAGCATCAACAAGGATGGC
ACAGTGTTCGACAGCCGCCCTATAGAGTCCCTGTCCCTGATAGTTGCTGTGATTCCTGATGTGGTGCAGA
TGCGGCAGCAGGCATTTGGAGAGAAGCTCACTCAGCAGCAAGCCAGTGCAGCAGCCACAGAGGCATCAGC
AATGGCAAAGGCGGCAACCATGACCCCAGCTGCTATTTCCCAGCAAAATCCTCCAAAGAACCGAGAGGCC
ACAGTGAACGGGGAAGAGAATGGAGCACATGAAATCAATAATCACTCAAAGCCAATGGAAATAGATGGGG
ATGTTGAGATTCCACCCAACAAAGCCACAGTCCTTCGGGGCCACGAGTCTGAGGTGTTCATTTGTGCCTG
GAACCCTGTCAGTGATTTGCTAGCCTCTGGATCTGGAGACTCAACTGCAAGGATATGGAACCTGAATGAG
AATAGCAACGGGGGCTCCACCCAGCTCGTGTTGAGACATTGTATACGAGAAGGGGGGCACGACGTCCCAA
GTAATAAAGATGTCACCTCACTGGACTGGAACAGTGATGGAACACTATTGGCTATGGGTTCATATGATGG
TTTCGCAAGAATATGGACAGAAAATGGTAACCTGGCCAGCACCTTAGGCCAACATAAAGGCCCCATCTTC
GCTCTGAAATGGAACAAAAAGGGGAATTATGTTTTGAGTGCTGGTGTAGACAAAACAACAATAATTTGGG
ATGCTCACACAGGAGAAGCCAAACAGCAGTTTCCTTTTCATTCAGCCCCCGCCCTTGATGTGGACTGGCA
GAACAACATGACCTTTGCCTCCTGTAGCACAGACATGTGTATCCATGTGTGCAGGCTCGGCTGTGACCAC
CCAGTCAAAACCTTCCAGGGACACACAAACGAGGTCAATGCCATCAAATGGGATCCTTCTGGAATGTTGC
TGGCGTCCTGCTCGGATGACATGACATTGAAGATCTGGAGTATGAAGCAGGATGCATGCGTCCACGATCT
TCAGGCTCACAGCAAAGAGATCTACACCATCAAGTGGAGCCCCACCGGGCCTGCCACCAGCAACCCAAAC
TCCAGCATCATGTTGGCAAGTGCTTCATTTGATTCTACAGTGCGACTGTGGGATGTGGAGCAAGGTGTCT
GCACCCACACACTCATGAAGCATCAAGAGCCTGTCTACAGTGTAGCTTTCAGCCCCGATGGAAAGTACTT
GGCTAGTGGATCCTTTGACAAGTATGTTCATATCTGGAATACTCAGAGTGGAAGTCTCGTCCACAGCTAC
CAAGGCACTGGCGGTATCTTCGAGGTGTGCTGGAACGCCCGAGGAGATAAAGTGGGCGCCAGCGCGTCTG
ATGGCTCTGTGTGTGTTCTGGATCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212240 protein sequence
Red=Cloning site Green=Tags(s)

MSITSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGTLVPPSALISILQKGLQYVEAEISINKDG
TVFDSRPIESLSLIVAVIPDVVQMRQQAFGEKLTQQQASAAATEASAMAKAATMTPAAISQQNPPKNREA
TVNGEENGAHEINNHSKPMEIDGDVEIPPNKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNE
NSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNSDGTLLAMGSYDGFARIWTENGNLASTLGQHKGPIF
ALKWNKKGNYVLSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNMTFASCSTDMCIHVCRLGCDH
PVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTLKIWSMKQDACVHDLQAHSKEIYTIKWSPTGPATSNPN
SSIMLASASFDSTVRLWDVEQGVCTHTLMKHQEPVYSVAFSPDGKYLASGSFDKYVHIWNTQSGSLVHSY
QGTGGIFEVCWNARGDKVGASASDGSVCVLDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134259
ORF Size 1566 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_134259.1, NP_599021.1
RefSeq Size 2312 bp
RefSeq ORF 1569 bp
Locus ID 90665
UniProt ID Q9BQ87
Domains WD40, LisH
Protein Families Transcription Factors
Protein Pathways Wnt signaling pathway
MW 56.7 kDa
Gene Summary The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. This gene is highly similar to TBL1X gene in nucleotide sequence and protein sequence, but the TBL1X gene is located on chromosome X and this gene is on chromosome Y. This gene has three alternatively spliced transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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