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CENTG3 (AGAP3) (NM_031946) Human Tagged ORF Clone
CAT＃: RC212440
AGAP3 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1
ORF Plasmid: tGFP
"NM_031946" in other vectors (4)
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CNY 6,672.00
CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AGAP-3; CENTG3; cnt-g3; CRAG; MRIP-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212440 representing NM_031946
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTCCAGGCGGGCGGGGGGCAGAGCCCGCAGCAGCAGCAGAGCCTGGCGGCTCCGGGGGGCGGCG GCGCTGCCGCGCAGCAGCTCGTCTGCGGCGGGCAGTTCGGCGGCGCGGGGCCCGGGGCCGGGGGCGGCGG CGGCCCCTCGCAGCAGCTGGCCGGCGGGCCCCCCCAGCAGTTCGCGCTCTCCAACTCCGCGGCCATCCGG GCCGAGATCCAGCGCTTCGAGTCCGTGCATCCCAATATCTACGCCATCTACGACCTGATCGAGCGCATCG AGGATTTGGCGCTGCAGAACCAGATCCGGGAGCACGTCATCTCCATCGAGGACTCGTTTGTGAACAGCCA GGAGTGGACGCTGAGCCGCTCCGTACCGGAGCTTAAAGTGGGCATAGTGGGGAACCTGTCTAGCGGGAAG TCAGCCCTGGTGCACCGCTATCTGACGGGGACCTATGTCCAGGAGGAGTCCCCTGAAGGGGGGCGGTTTA AGAAGGAGATTGTGGTGGATGGCCAGAGTTACCTGCTGCTGATCCGAGATGAAGGAGGCCCCCCTGAGCT CCAGTTTGCTGCCTGGGTGGATGCAGTGGTGTTTGTGTTCAGCCTGGAGGATGAAATCAGTTTCCAGACG GTGTACAACTACTTCCTGCGTCTCTGCAGCTTCCGCAACGCCAGCGAGGTGCCCATGGTGCTTGTGGGCA CGCAGGATGCCATCAGCGCTGCGAATCCCCGGGTTATCGACGACAGCAGAGCCCGCAAGCTCTCCACAGA TCTGAAGCGGTGCACCTACTATGAGACGTGCGCGACCTACGGGCTCAATGTGGAGCGTGTCTTCCAGGAC GTGGCCCAGAAGGTAGTGGCCTTGCGAAAGAAGCAGCAACTGGCCATCGGGCCCTGCAAGTCACTGCCCA ACTCGCCCAGCCACTCGGCCGTGTCCGCCGCCTCCATCCCGGCCGTGCACATCAACCAGGCCACGAATGG CGGCGGCAGCGCCTTCAGCGACTACTCGTCCTCAGTCCCCTCCACCCCCAGCATCAGCCAGCGGGAGCTG CGCATCGAGACCATCGCTGCCTCCTCCACCCCCACACCCATCCGAAAGCAGTCCAAGCGGCGCTCCAACA TCTTCACGTCTCGGAAGGGTGCTGACCTGGACCGGGAGAAGAAGGCTGCCGAGTGCAAGGTGGACAGCAT CGGGAGCGGCCGCGCCATCCCCATCAAGCAGGGGATCCTGCTAAAGCGGAGCGGCAAGTCCCTGAACAAG GAGTGGAAGAAGAAGTATGTGACGCTCTGTGACAACGGGCTGCTCACCTATCACCCCAGCCTGCATGATT ACATGCAGAACATCCACGGCAAGGAGATTGACCTGCTGCGGACAACGGTGAAAGTGCCAGGGAAGCGCCT GCCCCGAGCCACACCTGCCACAGCCCCGGGCACCAGCCCCCGTGCCAACGGGCTGTCCGTGGAGCGGAGT AACACACAGCTGGGTGGGGGCACAGGTGCCCCCCACTCGGCCAGCAGCGCATCCCTGCACTCTGAGCGCC CCCTCAGCAGCTCGGCCTGGGCTGGCCCGCGCCCTGAGGGGCTGCACCAGCGCTCCTGCTCCGTTTCCAG CGCCGACCAGTGGAGTGAGGCCACCACTTCCCTGCCCCCAGGCATGCAGCACCCTGCCAGTGGCCCAGCT GAGGTACTCAGTTCCAGCCCCAAGCTGGATCCTCCCCCATCTCCCCACTCCAACCGGAAGAAGCACCGGA GGAAAAAGAGCACCGGGACCCCCCGACCAGACGGCCCCAGCAGTGCTACTGAAGAGGCAGAGGAGTCGTT TGAATTTGTGGTGGTGTCCCTCACTGGGCAGACGTGGCACTTCGAGGCTTCAACGGCGGAGGAGCGGGAG CTGTGGGTTCAGAGTGTGCAGGCCCAGATCCTTGCCAGCCTGCAAGGCTGCCGCAGTGCCAAGGACAAGA CTCGACTGGGGAACCAGAACGCAGCTCTGGCTGTGCAGGCCGTCCGCACCGTCCGCGGCAACAGCTTTTG TATCGACTGCGATGCACCCAATCCAGACTGGGCCAGCCTGAACCTGGGTGCCCTGATGTGCATTGAGTGC TCAGGCATCCACCGACACCTGGGGGCTCACCTGTCCCGGGTGCGCTCCCTTGACCTCGATGACTGGCCGC CTGAGCTGCTGGCTGTCATGACTGCCATGGGCAATGCCCTCGCCAACAGCGTCTGGGAGGGGGCCTTGGG TGGCTACTCCAAGCCAGGGCCTGATGCCTGCAGAGAGGAGAAGGAACGCTGGATACGGGCCAAGTATGAA CAGAAGCTCTTCCTGGCCCCACTGCCAAGCTCAGATGTGCCACTGGGGCAGCAGCTGCTCCGGGCCGTGG TGGAAGATGACCTGCGGCTGTTGGTGATGCTCCTGGCACATGGCTCCAAAGAGGAGGTGAATGAGACCTA TGGGGACGGGGACGGGCGGACGGCTCTACATCTCTCCAGTGCCATGGCCAACGTTGTCTTCACGCAGCTG CTCATCTGGTACGGGGTGGACGTGAGGAGCCGGGACGCCCGGGGCCTGACTCCACTGGCATATGCTCGCC GGGCCGGCAGCCAGGAGTGTGCAGACATCTTGATCCAGCATGGCTGCCCTGGGGAGGGCTGTGGCTTAGC GCCTACCCCCAACAGAGAGCCTGCCAATGGCACCAACCCCTCTGCTGAGCTGCACCGTAGTCCTAGCCTC CTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212440 representing NM_031946
Red=Cloning site Green=Tags(s) MNFQAGGGQSPQQQQSLAAPGGGGAAAQQLVCGGQFGGAGPGAGGGGGPSQQLAGGPPQQFALSNSAAIR AEIQRFESVHPNIYAIYDLIERIEDLALQNQIREHVISIEDSFVNSQEWTLSRSVPELKVGIVGNLSSGK SALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPELQFAAWVDAVVFVFSLEDEISFQT VYNYFLRLCSFRNASEVPMVLVGTQDAISAANPRVIDDSRARKLSTDLKRCTYYETCATYGLNVERVFQD VAQKVVALRKKQQLAIGPCKSLPNSPSHSAVSAASIPAVHINQATNGGGSAFSDYSSSVPSTPSISQREL RIETIAASSTPTPIRKQSKRRSNIFTSRKGADLDREKKAAECKVDSIGSGRAIPIKQGILLKRSGKSLNK EWKKKYVTLCDNGLLTYHPSLHDYMQNIHGKEIDLLRTTVKVPGKRLPRATPATAPGTSPRANGLSVERS NTQLGGGTGAPHSASSASLHSERPLSSSAWAGPRPEGLHQRSCSVSSADQWSEATTSLPPGMQHPASGPA EVLSSSPKLDPPPSPHSNRKKHRRKKSTGTPRPDGPSSATEEAEESFEFVVVSLTGQTWHFEASTAEERE LWVQSVQAQILASLQGCRSAKDKTRLGNQNAALAVQAVRTVRGNSFCIDCDAPNPDWASLNLGALMCIEC SGIHRHLGAHLSRVRSLDLDDWPPELLAVMTAMGNALANSVWEGALGGYSKPGPDACREEKERWIRAKYE QKLFLAPLPSSDVPLGQQLLRAVVEDDLRLLVMLLAHGSKEEVNETYGDGDGRTALHLSSAMANVVFTQL LIWYGVDVRSRDARGLTPLAYARRAGSQECADILIQHGCPGEGCGLAPTPNREPANGTNPSAELHRSPSL L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031946 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031946.7 |
| RefSeq Size | 3247 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 116988 |
| UniProt ID | Q96P47 |
| Domains | ArfGap, PH, ANK, RAS, RAB |
| MW | 97.8 kDa |
| Gene Summary | This gene encodes an essential component of the N-methyl-D-aspartate (NMDA) receptor signaling complex which mediates long-term potentiation in synapses by linking activation of NMDA receptor to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The encoded protein contains an N-terminal GTPase-like domain, a pleckstrin homology domain, an ArfGAP domain and several C-terminal ankryn repeat domains. [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212440L3 | Lenti ORF clone of Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC212440L4 | Lenti ORF clone of Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RG212440 | AGAP3 (tGFP-tagged) - Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1 |
CNY 10,550.00 |
|
| SC318383 | AGAP3 (untagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1 |
CNY 6,680.00 |
