MST4 (STK26) (NM_016542) Human Tagged ORF Clone

CAT#: RC212607

MST4 (Myc-DDK-tagged)-Human serine/threonine protein kinase MST4 (MST4), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_016542" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


STK26 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms MASK; MST4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212607 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCACTCGCCGGTGGCTGTCCAAGTGCCTGGGATGCAGAATAACATAGCTGATCCAGAAGAACTGT
TCACAAAATTAGAGCGCATTGGGAAAGGCTCATTTGGGGAAGTTTTCAAAGGAATTGATAACCGTACCCA
GCAAGTCGTTGCTATTAAAATCATAGACCTTGAGGAAGCCGAAGATGAAATAGAAGACATTCAGCAAGAA
ATAACTGTCTTGAGTCAATGTGACAGCTCATATGTAACAAAATACTATGGGTCATATTTAAAGGGGTCTA
AATTATGGATAATAATGGAATACCTGGGCGGTGGTTCAGCACTGGATCTTCTTCGAGCTGGTCCATTTGA
TGAGTTCCAGATTGCTACCATGCTAAAGGAAATTTTAAAAGGTCTGGACTATCTGCATTCAGAAAAGAAA
ATTCACCGAGACATAAAAGCTGCCAATGTCTTGCTCTCAGAACAAGGAGATGTTAAACTTGCTGATTTTG
GAGTTGCTGGTCAGCTGACAGATACACAGATTAAAAGAAATACCTTTGTGGGAACTCCATTTTGGATGGC
TCCTGAAGTTATTCAACAGTCAGCTTATGACTCAAAAGCTGACATTTGGTCATTGGGAATTACTGCTATT
GAACTAGCCAAGGGAGAGCCACCTAACTCCGATATGCATCCAATGAGAGTTCTGTTTCTTATTCCCAAAA
ACAATCCTCCAACTCTTGTTGGAGACTTTACTGAGTCTTTTAAGGAGTTTATTGATGCTTGCCTGAACAA
AGATCCATCATTTCGTCCTACAGCAAAAGAACTTCTGAAACACAAATTCATTGTAAAAAATTCAAAGAAG
ACTTCTTATCTGACTGAACTGATAGATCGTTTTAAGAGATGGAAGGCAGAAGGACACAGTGATGATGAAT
CTGATTCCGAGGGCTCTGATTCGGAATCTACCAGCAGGGAAAACAATACTCATCCTGAATGGAGCTTTAC
CACCGTACGAAAGAAGCCTGATCCAAAGAAAGTACAGAATGGGGCAGAGCAAGATCTTGTGCAAACCCTG
AGTTGTTTGTCTATGATAATCACACCTGCATTTGCTGAACTTAAACAGCAGGACGAGAATAACGCTAGCA
GGAATCAGGCGATTGAAGAACTCGAGAAAAGTATTGCTGTGGCTGAAGCCGCCTGTCCCGGCATCACAGA
TAAAATGGTGAAGAAACTAATTGAAAAATTTCAAAAGTGTTCAGCAGACGAATCCCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212607 protein sequence
Red=Cloning site Green=Tags(s)

MAHSPVAVQVPGMQNNIADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQE
ITVLSQCDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKK
IHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGITAI
ELAKGEPPNSDMHPMRVLFLIPKNNPPTLVGDFTESFKEFIDACLNKDPSFRPTAKELLKHKFIVKNSKK
TSYLTELIDRFKRWKAEGHSDDESDSEGSDSESTSRENNTHPEWSFTTVRKKPDPKKVQNGAEQDLVQTL
SCLSMIITPAFAELKQQDENNASRNQAIEELEKSIAVAEAACPGITDKMVKKLIEKFQKCSADESP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016542
ORF Size 1248 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016542.4
RefSeq Size 3352 bp
RefSeq ORF 1251 bp
Locus ID 51765
UniProt ID Q9P289
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
MW 46.5 kDa
Gene Summary The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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