BAG5 (NM_001015048) Human Tagged ORF Clone

CAT#: RC212765

BAG5 (Myc-DDK-tagged)-Human BCL2-associated athanogene 5 (BAG5), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001015048" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


BAG5 mouse monoclonal antibody,clone OTI2D6
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BAG-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212765 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTTTCCATTGGTTACCCACCTTGAGCGAGCCGTTTGATAGAAACCAAGAGCTTGAAACGTGCATTA
GGCCGTTGTGGACTCCCAGTGGGAGTGCTTGTGAAACTGAACACAACAAAAGTATGGATATGGGAAACCA
ACATCCTTCTATTAGTAGGCTTCAGGAAATCCAAAAGGAAGTAAAAAGTGTAGAACAGCAAGTTATCGGC
TTCAGTGGTCTGTCAGATGACAAGAATTACAAGAAACTGGAGAGGATTCTAACAAAACAGCTTTTTGAAA
TAGACTCTGTAGATACTGAAGGAAAAGGAGATATTCAGCAAGCTAGGAAGCGGGCAGCACAGGAGACAGA
ACGTCTTCTCAAAGAGTTGGAGCAGAATGCAAACCACCCACACCGGATTGAAATACAGAACATTTTTGAG
GAAGCCCAGTCCCTCGTGAGAGAGAAAATTGTGCCATTTTATAATGGAGGCAACTGCGTAACTGATGAGT
TTGAAGAAGGCATCCAAGATATCATTCTGAGGCTGACACATGTTAAAACTGGAGGAAAAATCTCCTTGCG
GAAAGCAAGGTATCACACTTTAACCAAAATCTGTGCGGTGCAAGAGATAATCGAAGACTGCATGAAAAAG
CAGCCTTCCCTGCCGCTTTCCGAGGATGCACATCCTTCCGTTGCCAAAATCAACTTCGTGATGTGTGAGG
TGAACAAGGCCCGAGGGGTCCTGATTGCACTTCTGATGGGTGTGAACAACAATGAGACCTGCAGGCACTT
ATCCTGTGTGCTCTCGGGGCTGATCGCTGACCTGGATGCTCTAGATGTGTGCGGCCGGACAGAAATCAGA
AATTATCGGAGGGAGGTAGTAGAAGATATCAACAAATTATTGAAATATCTGGATTTGGAAGAGGAAGCAG
ACACAACTAAAGCATTTGACCTGAGACAGAATCATTCCATTTTAAAAATAGAAAAGGTCCTCAAGAGAAT
GAGAGAAATAAAAAATGAACTTCTCCAAGCACAAAACCCTTCTGAATTGTACCTGAGCTCCAAAACAGAA
TTGCAGGGTTTAATTGGACAGTTGGATGAGGTAAGTCTTGAAAAAAACCCCTGCATCCGGGAAGCCAGGA
GAAGAGCAGTGATCGAGGTGCAAACTCTGATCACATATATTGACTTGAAGGAGGCCCTTGAGAAAAGAAA
GCTGTTTGCTTGTGAGGAGCACCCATCCCATAAAGCCGTCTGGAACGTCCTTGGAAACTTGTCTGAGATC
CAGGGAGAAGTTCTTTCATTTGATGGAAATCGAACCGATAAGAACTACATCCGGCTGGAAGAGCTGCTCA
CCAAGCAGCTGCTAGCCCTGGATGCTGTTGATCCGCAGGGAGAAGAGAAGTGTAAGGCTGCCAGGAAACA
AGCTGTGAGGCTTGCGCAGAATATTCTCAGCTATCTCGACCTGAAATCTGATGAATGGGAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212765 protein sequence
Red=Cloning site Green=Tags(s)

MRFHWLPTLSEPFDRNQELETCIRPLWTPSGSACETEHNKSMDMGNQHPSISRLQEIQKEVKSVEQQVIG
FSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFE
EAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKICAVQEIIEDCMKK
QPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIR
NYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTE
LQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALEKRKLFACEEHPSHKAVWNVLGNLSEI
QGEVLSFDGNRTDKNYIRLEELLTKQLLALDAVDPQGEEKCKAARKQAVRLAQNILSYLDLKSDEWEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001015048
ORF Size 1467 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq Size 4866 bp
RefSeq ORF 1344 bp
Locus ID 9529
UniProt ID Q9UL15
Protein Families Druggable Genome
MW 56 kDa
Gene Summary The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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