HDAC5 (NM_005474) Human Tagged Lenti ORF Clone
CAT#: RC212863L3
- LentiORF®
Lenti-ORF clone of HDAC5 (Myc-DDK-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1
"NM_005474" in other vectors (6)
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CNY 14,120.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HD5; NY-CO-9 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC212863).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_005474 |
ORF Size | 3366 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005474.4 |
RefSeq Size | 5324 bp |
RefSeq ORF | 3369 bp |
Locus ID | 10014 |
UniProt ID | Q9UQL6 |
Domains | Hist_deacetyl |
Protein Families | Druggable Genome, Transcription Factors |
MW | 121.8 kDa |
Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212863 | HDAC5 (Myc-DDK-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1 |
CNY 11,720.00 |
|
RC212863L1 | Lenti-ORF clone of HDAC5 (Myc-DDK-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1 |
CNY 14,120.00 |
|
RC212863L2 | Lenti-ORF clone of HDAC5 (mGFP-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1 |
CNY 14,120.00 |
|
RC212863L4 | Lenti-ORF clone of HDAC5 (mGFP-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1 |
CNY 14,120.00 |
|
RG212863 | HDAC5 (tGFP-tagged) - Human histone deacetylase 5 (HDAC5), transcript variant 1 |
CNY 13,320.00 |
|
SC124229 | HDAC5 (untagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1 |
CNY 11,728.00 |