Transglutaminase 7 (TGM7) (NM_052955) Human Tagged ORF Clone

CAT#: RC213082

TGM7 (Myc-DDK-tagged)-Human transglutaminase 7 (TGM7)

ORF Plasmid: DDK tGFP



  "NM_052955" in other vectors (4)

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CNY 7,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-TGM7 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms TGMZ
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213082 representing NM_052955
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCAGGTGGCAACCTTGCGGCTTGAGTCTGTCGACCTGCAGAGCTCCAGGAACAACAAGGAGCACC
ACACGCAGGAGATGGGCGTCAAGCGGCTCACTGTGCGCCGCGGCCAGCCCTTCTACCTCCGGCTGAGCTT
CAGCCGACCCTTCCAGTCCCAGAACGACCACATCACCTTTGTGGCTGAGACCGGACCCAAGCCGTCAGAG
CTGCTGGGGACCCGAGCCACATTCTTCCTCACCCGGGTCCAGCCCGGGAATGTCTGGAGCGCTTCTGATT
TCACCATTGACTCCAACTCTCTCCAAGTTTCCCTTTTCACACCAGCCAATGCAGTTATTGGCCATTACAC
TCTGAAAATAGAGATCTCTCAGGGCCAAGGTCACAGTGTGACTTACCCGCTGGGAACTTTCATCCTACTT
TTTAACCCTTGGAGTCCAGAGGACGACGTCTACCTGCCAAGTGAAATACTGCTGCAGGAGTATATCATGC
GAGATTATGGCTTTGTTTACAAGGGTCATGAAAGATTCATCACCTCCTGGCCCTGGAACTACGGGCAGTT
TGAAGAGGACATCATAGACATCTGCTTTGAGATCCTGAACAAGAGCCTGTATCACTTAAAGAACCCGGCC
AAAGACTGTTCCCAGCGGAACGACGTGGTGTATGTGTGCAGGGTGGTGAGTGCCATGATCAACAGCAACG
ATGACAATGGCGTGCTGCAGGGGAACTGGGGCGAGGACTACTCCAAAGGGGTCAGTCCTCTGGAGTGGAA
GGGCAGTGTGGCCATCCTACAGCAGTGGTCAGCCAGGGGCGGGCAGCCTGTGAAGTACGGACAGTGCTGG
GTCTTCGCCTCTGTTATGTGCACCGTAATGAGATGCTTAGGTGTTCCAACCCGTGTTGTTTCCAATTTCC
GTTCCGCGCACAACGTGGATAGGAACTTGACCATCGATACGTACTATGACCGAAATGCCGAGATGCTGTC
AACTCAGAAACGAGACAAAATATGGAACTTCCACGTCTGGAATGAGTGCTGGATGATCCGGAAAGATCTC
CCACCAGGATACAACGGGTGGCAGGTTCTGGACCCCACTCCCCAGCAGACCAGCAGTGGGCTGTTCTGCT
GTGGCCCTGCCTCTGTGAAGGCCATCAGGGAAGGGGATGTCCACCTGGCCTATGACACCCCTTTTGTGTA
TGCCGAGGTGAACGCCGATGAAGTCATTTGGCTCCTTGGGGATGGCCAGGCCCAGGAAATCCTGGCCCAC
AACACCAGTTCCATCGGGAAGGAGATCAGCACTAAGATGGTGGGGTCAGACCAGCGCCAGAGCATCACCA
GCTCCTACAAGTACCCAGAAGGATCCCCTGAGGAGAGAGCTGTCTTCATGAAGGCTTCTCGGAAAATGCT
GGGCCCCCAAAGAGCTTCTTTGCCCTTCCTGGATCTCCTGGAGTCTGGGGGTCTTAGGGATCAGCCAGCG
CAGCTGCAGCTTCACCTGGCCAGGATACCCGAGTGGGGCCAGGACCTGCAGCTGCTGCTGCGTATCCAGA
GGGTGCCAGACAGCACCCACCCTCGGGGGCCCATCGGACTGGTGGTGCGCTTCTGTGCACAGGCCCTGCT
GCATGGGGGTGGTACCCAGAAGCCCTTCTGGAGGCACACAGTGCGGATGAACCTGGACTTTGGGAAGGAG
ACACAGTGGCCGCTCCTCCTGCCCTACAGCAATTACAGAAACAAGCTAACGGACGAAAAGCTCATCCGCG
TGTCTGGCATCGCGGAGGTTGAAGAGACAGGGAGGTCCATGCTGGTCCTAAAAGATATCTGTCTGGAGCC
TCCCCACTTGTCTATTGAGGTGTCTGAGAGGGCTGAGGTGGGCAAGGCGCTGAGAGTCCATGTCACCCTC
ACCAACACCTTAATGGTGGCTCTGAGCAGCTGCACGATGGTGCTGGAAGGAAGCGGCCTCATCAATGGGC
AGATAGCAAAGGACCTTGGGACTCTGGTGGCCGGACACACCCTCCAAATTCAACTGGACCTCTACCCGAC
CAAAGCTGGACCCCGCCAGCTCCAGGTTCTCATCAGCAGCAACGAGGTCAAGGAGATCAAAGGCTACAAG
GACATCTTCGTCACTGTGGCTGGGGCTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213082 representing NM_052955
Red=Cloning site Green=Tags(s)

MDQVATLRLESVDLQSSRNNKEHHTQEMGVKRLTVRRGQPFYLRLSFSRPFQSQNDHITFVAETGPKPSE
LLGTRATFFLTRVQPGNVWSASDFTIDSNSLQVSLFTPANAVIGHYTLKIEISQGQGHSVTYPLGTFILL
FNPWSPEDDVYLPSEILLQEYIMRDYGFVYKGHERFITSWPWNYGQFEEDIIDICFEILNKSLYHLKNPA
KDCSQRNDVVYVCRVVSAMINSNDDNGVLQGNWGEDYSKGVSPLEWKGSVAILQQWSARGGQPVKYGQCW
VFASVMCTVMRCLGVPTRVVSNFRSAHNVDRNLTIDTYYDRNAEMLSTQKRDKIWNFHVWNECWMIRKDL
PPGYNGWQVLDPTPQQTSSGLFCCGPASVKAIREGDVHLAYDTPFVYAEVNADEVIWLLGDGQAQEILAH
NTSSIGKEISTKMVGSDQRQSITSSYKYPEGSPEERAVFMKASRKMLGPQRASLPFLDLLESGGLRDQPA
QLQLHLARIPEWGQDLQLLLRIQRVPDSTHPRGPIGLVVRFCAQALLHGGGTQKPFWRHTVRMNLDFGKE
TQWPLLLPYSNYRNKLTDEKLIRVSGIAEVEETGRSMLVLKDICLEPPHLSIEVSERAEVGKALRVHVTL
TNTLMVALSSCTMVLEGSGLINGQIAKDLGTLVAGHTLQIQLDLYPTKAGPRQLQVLISSNEVKEIKGYK
DIFVTVAGAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_052955
ORF Size 2130 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_052955.3
RefSeq Size 2312 bp
RefSeq ORF 2133 bp
Locus ID 116179
UniProt ID Q96PF1
Protein Families Druggable Genome
MW 79.8 kDa
Gene Summary Transglutaminases (TGM; EC 2.3.2.13) are a family of structurally and functionally related enzymes that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. For additional background information on transglutaminases, see TGM1 (MIM 190195).[supplied by OMIM, Jul 2002]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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