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ZNF185 (NM_007150) Human Tagged ORF Clone
CAT＃: RC213230
ZNF185 (Myc-DDK-tagged)-Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 4
ORF Plasmid: tGFP
"NM_007150" in other vectors (4)
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CNY 5,048.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SCELL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213230 representing NM_007150
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATCTCAGCTCTTGGAGGCCGCACCAAAGGGAAGCCTCTGCCACCAGGCGAGGAGGAGCGCAATA ACGTTCTCAAGCAGATGAAAGTGCGAACCACGCTGAAGGGGGACAAGAGCTGGATTACCAAGCAGGATGA ATCGGAGGGTCGCACCATAGAGCTGCCCTCAGGCCGGAGTCGCGCCACATCCTTTTCATCAGCTGGGGAG GTTCCGAAGCCTAGGCCTCCGAGCACAAGGGCTCCCACTGGCTACATCATCCGGGGAGTGTTCACCAAGC CCATAGACAGCTCTTCCCAGCCCCAGCAGCAGTTCCCCAAGGCCAACGGGACTCCAAAAAGTGCTGCCAG TCTGGTGAGAACAGCTAACGCTGGTCCTCCCCGCCCCTCCTCCTCTGGCTACAAGATGACCACTGAGGAT TACAAGAAGCTGGCACCCTACAATATCAGGCGCAGCTCTACATCAGGGGACACCGAGGAGGAGGAGGAGG AGGAGGTGGTGCCATTCTCCTCAGATGAACAGAAACGGAGGTCAGAGGCTGCAAGCGGTGTTCTGAGGAG GACAGCTCCCCGGGAGCACTCCTACGTCCTGTCAGCGGCCAAGAAGAGCACTGGTCCTACCCAGGAGACA CAGGCACCGTTTATCGCGAAGAGGGTGGAGGTGGTGGAAGAGGACGGGCCTTCTGAGAAGAGCCAGGACC CACCTGCTCTGGCAAGATCCACTCCTGGCTCAAACAGTGCGGATGGAGGCAGGACCAAAGCGTCTCGGGC AATTTGGATCGAGTGCCTGCCAAGTATGCCTAGCCCCGCTGGGAGCCAGGAGCTCAGCTCAAGAGGTGAG GAAATTGTCCGCCTGCAGATCCTGACACCCAGGGCAGGACTCCGCCTGGTGGCCCCAGACGTGGAAGGCA TGAGGTCTTCCCCAGGCAACAAAGACAAGGAGGCCCCCTGCTCCAGAGAGCTCCAGAGGGACTTGGCTGG TGAGGAGGCTTTCAGGGCCCCCAACACAGATGCTGCAAGGTCAAGTGCACAGTTGAGTGATGGCAATGTG GGATCCGGAGCCACGGGCTCCCGGCCTGAAGGCTTGGCTGCAGTAGACATCGGCTCCGAGAGAGGAAGCT CCAGTGCCACTTCAGTCTCTGCTGTCCCTGCTGATAGGAAGAGCAACAGCACAGCAGCCCAGGAGGATGC AAAGGCAGACCCAAAGGGGGCCTTGGCTGATTATGAGGGGAAGGATGTGGCCACCAGGGTCGGAGAGGCC TGGCAGGAGAGGCCTGGAGCTCCAAGAGGTGGCCAAGGAGACCCAGCTGTACCCGCTCAGCAACCTGCAG ATCCCAGCACCCCAGAGCGGCAGAGCAGCCCCAGCGGATCTGAGCAACTTGTCAGACGAGAGAGTTGTGG CAGCAGCGTGTTGACTGATTTTGAGGGGAAGGATGTGGCCACCAAGGTCGGAGAGGCCTGGCAGGACAGG CCTGGAGCCCCAAGAGGTGGCCAAGGAGACCCAGCTGTACCCACTCAGCAACCTGCAGATCCCAGTACCC CAGAACAGCAGAACAGCCCCAGCGGATCTGAGCAATTCGTCAGACGAGAGAGCTGCACCAGCAGGGTGAG GAGCCCCTCGAGCTGCATGGTCACTGTTACTGTCACTGCCACATCTGAGCAGCCTCACATTTATATTCCA GCCCCCGCAAGTGAATTGGACTCCAGCTCTACCACCAAAGGGATTCTCTTCGTGAAGGAGTACGTGAATG CTAGTGAAGTGTCTTCTGGGAAGCCAGTATCTGCACGCTATAGCAACGTCAGCAGCATTGAGGACTCATT CGCCATGGAGAAGAAGCCTCCATGTGGCAGCACTCCATACTCTGAGAGGACAACTGGAGGGATCTGTACT TACTGCAACCGTGAGATCCGAGACTGTCCAAAGATTACCCTAGAACATCTTGGTATCTGCTGCCATGAAT ATTGCTTTAAGTGTGGGATTTGCAGTAAACCGATGGGCGATCTCCTGGATCAGATCTTCATTCACCGTGA CACCATTCACTGTGGGAAATGCTATGAGAAGCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213230 representing NM_007150
Red=Cloning site Green=Tags(s) MSISALGGRTKGKPLPPGEEERNNVLKQMKVRTTLKGDKSWITKQDESEGRTIELPSGRSRATSFSSAGE VPKPRPPSTRAPTGYIIRGVFTKPIDSSSQPQQQFPKANGTPKSAASLVRTANAGPPRPSSSGYKMTTED YKKLAPYNIRRSSTSGDTEEEEEEEVVPFSSDEQKRRSEAASGVLRRTAPREHSYVLSAAKKSTGPTQET QAPFIAKRVEVVEEDGPSEKSQDPPALARSTPGSNSADGGRTKASRAIWIECLPSMPSPAGSQELSSRGE EIVRLQILTPRAGLRLVAPDVEGMRSSPGNKDKEAPCSRELQRDLAGEEAFRAPNTDAARSSAQLSDGNV GSGATGSRPEGLAAVDIGSERGSSSATSVSAVPADRKSNSTAAQEDAKADPKGALADYEGKDVATRVGEA WQERPGAPRGGQGDPAVPAQQPADPSTPERQSSPSGSEQLVRRESCGSSVLTDFEGKDVATKVGEAWQDR PGAPRGGQGDPAVPTQQPADPSTPEQQNSPSGSEQFVRRESCTSRVRSPSSCMVTVTVTATSEQPHIYIP APASELDSSSTTKGILFVKEYVNASEVSSGKPVSARYSNVSSIEDSFAMEKKPPCGSTPYSERTTGGICT YCNREIRDCPKITLEHLGICCHEYCFKCGICSKPMGDLLDQIFIHRDTIHCGKCYEKLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007150 |
| ORF Size | 2067 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007150.3, NP_009081.2 |
| RefSeq Size | 4312 bp |
| RefSeq ORF | 2070 bp |
| Locus ID | 7739 |
| UniProt ID | O15231 |
| Domains | LIM |
| MW | 73.3 kDa |
| Gene Summary | Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This gene encodes a LIM-domain zinc finger protein. The LIM domain is composed of two contiguous zinc finger domains, separated by a two-amino acid residue hydrophobic linker. The LIM domain mediates protein:protein interactions. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213230L3 | Lenti-ORF clone of ZNF185 (Myc-DDK-tagged)-Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 4 |
CNY 7,700.00 |
|
| RC213230L4 | Lenti-ORF clone of ZNF185 (mGFP-tagged)-Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 4 |
CNY 7,700.00 |
|
| RG213230 | ZNF185 (tGFP-tagged) - Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 4 |
CNY 6,370.00 |
|
| SC318167 | ZNF185 (untagged)-Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 4 |
CNY 13,300.00 |
