Plexin B2 (PLXNB2) (NM_012401) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	dJ402G11.3; MM1; Nbla00445; PLEXB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC213651 representing NM_012401. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCACTGCAGCTCTGGGCCCTGACCCTGCTGGGCCTGCTGGGCGCAGGTGCCAGCCTGAGGCCCCGC AAGCTGGACTTCTTCCGCAGCGAGAAAGAGCTGAACCACCTGGCTGTGGATGAGGCCTCAGGCGTGGTG TACCTGGGGGCGGTGAATGCCCTCTACCAGCTGGATGCGAAGCTGCAGCTGGAGCAGCAGGTGGCCACG GGCCCGGCCCTGGACAACAAGAAGTGCACGCCGCCCATCGAGGCCAGCCAGTGCCATGAGGCTGAGATG ACTGACAATGTCAACCAGCTGCTGCTGCTCGACCCTCCCAGGAAGCGCCTGGTGGAGTGCGGCAGCCTC TTCAAGGGCATCTGCGCTCTGCGCGCCCTGAGCAACATCTCCCTCCGCCTGTTCTACGAGGACGGCAGC GGGGAGAAGTCTTTCGTGGCCAGCAATGATGAGGGCGTGGCCACAGTGGGGCTGGTGAGCTCCACGGGT CCTGGTGGTGACCGCGTGCTGTTTGTGGGCAAAGGCAATGGGCCACACGACAACGGCATCATCGTGAGC ACTCGGCTGTTGGACCGGACTGACAGCAGGGAGGCCTTTGAAGCCTACACGGACCACGCCACCTACAAG GCCGGCTACCTGTCCACCAACACACAGCAGTTCGTGGCGGCCTTCGAGGACGGCCCCTACGTCTTCTTT GTCTTCAACCAGCAGGACAAGCACCCGGCCCGGAACCGCACGCTGCTGGCACGCATGTGCAGAGAAGAC CCCAACTACTACTCCTACCTGGAGATGGACCTGCAGTGCCGGGACCCCGACATCCACGCCGCTGCCTTT GGCACCTGCCTGGCCGCCTCCGTGGCTGCGCCTGGCTCTGGCAGGGTGCTATATGCTGTCTTCAGCAGA GACAGCCGGAGCAGTGGGGGGCCCGGTGCGGGCCTCTGCCTGTTCCCGCTGGACAAGGTGCACGCCAAG ATGGAGGCCAACCGCAACGCCTGTTACACAGGCACCCGGGAGGCCCGTGACATCTTCTACAAGCCCTTC CACGGCGATATCCAGTGCGGCGGCCACGCGCCGGGCTCCAGCAAGAGCTTCCCATGTGGCTCGGAGCAC CTGCCCTACCCGCTGGGCAGCCGCGACGGGCTCAGAGGCACAGCCGTGCTGCAGCGTGGAGGCCTGAAC CTCACGGCCGTGACGGTCGCCGCCGAGAACAACCACACTGTTGCTTTTCTGGGCACCTCTGATGGCCGG ATCCTCAAGGTGTACCTCACCCCAGATGGCACCTCCTCAGAGTACGACTCTATCCTTGTGGAGATAAAC AAGAGAGTCAAGCGCGACCTGGTACTGTCTGGAGACCTGGGCAGCCTGTACGCCATGACCCAGGACAAG GTGTTCCGGCTGCCGGTGCAGGAGTGCCTGAGCTACCCGACCTGCACCCAGTGCCGCGACTCCCAGGAC CCCTACTGCGGCTGGTGCGTCGTCGAGGGACGATGCACCCGGAAGGCCGAGTGTCCGCGGGCCGAGGAG GCCAGCCACTGGCTGTGGAGCCGAAGCAAGTCCTGCGTGGCCGTCACCAGCGCCCAGCCACAGAACATG AGCCGGCGGGCCCAGGGGGAGGTGCAGCTGACCGTCAGCCCCCTCCCTGCCCTGAGCGAGGAGGACGAG TTGCTGTGCCTTTTTGGGGAGTCGCCGCCACACCCCGCCCGCGTGGAGGGCGAGGCCGTCATCTGCAAC TCCCCAAGCAGCATCCCCGTCACACCGCCAGGCCAGGACCACGTGGCCGTGACCATCCAGCTCCTCCTT AGACGAGGCAACATCTTCCTCACGTCCTACCAGTACCCCTTCTACGACTGCCGCCAGGCCATGAGCCTG GAGGAGAACCTGCCGTGCATCTCCTGCGTGAGCAACCGCTGGACCTGCCAGTGGGACCTGCGCTACCAC GAGTGCCGGGAGGCTTCGCCCAACCCTGAGGACGGCATCGTCCGTGCCCACATGGAGGACAGCTGTCCC CAGTTCCTGGGACCCAGCCCCCTGGTGATCCCCATGAACCACGAGACAGATGTGAACTTCCAGGGCAAG AACCTGGACACCGTGAAGGGTTCCTCCCTGCACGTGGGCAGTGACTTGCTCAAGTTCATGGAGCCGGTG ACCATGCAGGAATCTGGGACCTTCGCCTTTCGGACCCCAAAGCTGTCCCACGATGCCAACGAGACGCTG CCCCTGCACCTCTACGTCAAGTCTTACGGCAAGAATATCGACAGCAAGCTCCATGTGACCCTCTACAAC TGCTCCTTTGGCCGCAGCGACTGCAGCCTGTGCCGGGCCGCTAACCCCGACTACAGGTGTGCGTGGTGC GGGGGCCAGAGCAGGTGCGTGTATGAGGCCCTGTGCAACACCACCTCCGAGTGCCCGCCGCCCGTCATC ACCAGGATCCAGCCTGAGACGGGCCCCCTGGGTGGGGGCATCCGCATCACCATCCTGGGGTCCAATTTG GGCGTCCAAGCAGGGGACATCCAGAGGATCTCTGTGGCCGGCCGGAACTGCTCCTTTCAGCCGGAACGT TACTCCGTGTCCACCCGGATCGTGTGTGTGATCGAGGCTGCGGAGACGCCTTTCACGGGGGGTGTCGAG GTGGACGTCTTCGGGAAACTGGGCCGTTCGCCTCCCAATGTCCAGTTCACCTTCCAACAGCCCAAGCCT CTCAGTGTGGAGCCGCAGCAGGGACCGCAGGCGGGCGGCACCACACTGACCATCCACGGCACCCACCTG GACACGGGCTCCCAGGAGGACGTGCGGGTGACCCTCAACGGCGTCCCGTGTAAAGTGACGAAGTTTGGG GCGCAGCTCCAGTGTGTCACTGGCCCCCAGGCGACACGGGGCCAGATGCTTCTGGAGGTCTCCTACGGG GGGTCCCCCGTGCCCAACCCCGGCATCTTCTTCACCTACCGCGAAAACCCCGTACTGCGAGCCTTCGAG CCGCTACGAAGCTTTGCCAGTGGTGGCCGCAGCATCAACGTCACGGGTCAGGGCTTCAGCCTGATCCAG AGGTTTGCCATGGTGGTCATCGCGGAGCCCCTGCAGTCCTGGCAGCCGCCGCGGGAGGCTGAATCCCTG CAGCCCATGACGGTGGTGGGTACAGACTACGTGTTCCACAATGACACCAAGGTCGTCTTCCTGTCCCCG GCTGTGCCTGAGGAGCCAGAGGCCTACAACCTCACGGTGCTGATCGAGATGGACGGGCACCGTGCCCTG CTCAGAACAGAGGCCGGGGCCTTCGAGTACGTGCCTGACCCCACCTTTGAGAACTTCACAGGTGGCGTC AAGAAGCAGGTCAACAAGCTCATCCACGCCCGGGGCACCAATCTGAACAAGGCGATGACGCTGCAGGAG GCCGAGGCCTTCGTGGGTGCCGAGCGCTGCACCATGAAGACGCTGACGGAGACCGACCTGTACTGTGAG CCCCCGGAGGTGCAGCCCCCGCCCAAGCGGCGGCAGAAACGAGACACCACACACAACCTGCCCGAGTTC ATTGTGAAGTTCGGCTCTCGCGAGTGGGTGCTGGGCCGCGTGGAGTACGACACACGGGTGAGCGACGTG CCGCTCAGCCTCATCTTGCCGCTGGTCATCGTGCCCATGGTGGTCGTCATCGCGGTGTCTGTCTACTGC TACTGGAGGAAGAGCCAGCAGGCCGAACGAGAGTATGAGAAGATCAAGTCCCAGCTGGAGGGCCTGGAG GAGAGCGTGCGGGACCGCTGCAAGAAGGAATTCACAGACCTGATGATCGAGATGGAGGACCAGACCAAC GACGTGCACGAGGCCGGCATCCCCGTGCTGGACTACAAGACCTACACCGACCGCGTCTTCTTCCTGCCC TCCAAGGACGGCGACAAGGACGTGATGATCACCGGCAAGCTGGACATCCCTGAGCCGCGGCGGCCGGTG GTGGAGCAGGCCCTCTACCAGTTCTCCAACCTGCTGAACAGCAAGTCTTTCCTCATCAATTTCATCCAC ACCCTGGAGAACCAGCGGGAGTTCTCGGCCCGCGCCAAGGTCTACTTCGCGTCCCTGCTGACGGTGGCG CTGCACGGGAAACTGGAGTACTACACGGACATCATGCACACGCTCTTCCTGGAGCTCCTGGAGCAGTAC GTGGTGGCCAAGAACCCCAAGCTGATGCTGCGCAGGTCTGAGACTGTGGTGGAGAGGATGCTGTCCAAC TGGATGTCCATCTGCCTGTACCAGTACCTCAAGGACAGTGCCGGGGAGCCCCTGTACAAGCTCTTCAAG GCCATCAAACATCAGGTGGAAAAGGGCCCGGTGGATGCGGTACAGAAGAAGGCCAAGTACACTCTCAAC GACACGGGGCTGCTGGGGGATGATGTGGAGTACGCACCCCTGACGGTGAGCGTGATCGTGCAGGACGAG GGAGTGGACGCCATCCCGGTGAAGGTCCTCAACTGTGACACCATCTCCCAGGTCAAGGAGAAGATCATT GACCAGGTGTACCGTGGGCAGCCCTGCTCCTGCTGGCCCAGGCCAGACAGCGTGGTCCTGGAGTGGCGT CCGGGCTCCACAGCGCAGATCCTGTCGGACCTGGACCTGACGTCACAGCGGGAGGGCCGGTGGAAGCGC GTCAACACCCTTATGCACTACAATGTCCGGGATGGAGCCACCCTCATCCTGTCCAAGGTGGGGGTCTCC CAGCAGCCGGAGGACAGCCAGCAGGACCTGCCTGGGGAGCGCCATGCCCTCCTGGAGGAGGAGAACCGG GTGTGGCACCTGGTGCGGCCGACCGACGAGGTGGACGAGGGCAAGTCCAAGAGAGGCAGCGTGAAAGAG AAGGAGCGGACGAAGGCCATCACCGAGATCTACCTGACGCGGCTGCTCTCAGTCAAGGGCACACTGCAG CAGTTTGTGGACAACTTCTTCCAGAGCGTGCTGGCGCCTGGGCACGCGGTGCCACCTGCAGTCAAGTAC TTCTTCGACTTCCTGGACGAGCAGGCAGAGAAGCACAACATCCAGGATGAAGACACCATCCACATCTGG AAGACGAACAGCTTACCGCTCCGGTTCTGGGTGAACATCCTCAAGAACCCCCACTTCATCTTTGACGTG CATGTCCACGAGGTGGTGGACGCCTCGCTGTCAGTCATCGCGCAGACCTTCATGGATGCCTGCACGCGC ACGGAGCATAAGCTGAGCCGCGATTCTCCCAGCAACAAGCTGCTGTACGCCAAGGAGATCTCCACCTAC AAGAAGATGGTGGAGGATTACTACAAGGGGATCCGGCAGATGGTGCAGGTCAGCGACCAGGACATGAAC ACACACCTGGCAGAGATTTCCCGGGCGCACACGGACTCCTTGAACACCCTCGTGGCACTCCACCAGCTC TACCAATACACGCAGAAGTACTATGACGAGATCATCAATGCCTTGGAGGAGGATCCTGCCGCCCAGAAG ATGCAGCTGGCCTTCCGCCTGCAGCAGATTGCCGCTGCACTGGAGAACAAGGTCACTGACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC213651 Blue=ORF Red=Cloning site Green=Tag(s) MALQLWALTLLGLLGAGASLRPRKLDFFRSEKELNHLAVDEASGVVYLGAVNALYQLDAKLQLEQQVAT GPALDNKKCTPPIEASQCHEAEMTDNVNQLLLLDPPRKRLVECGSLFKGICALRALSNISLRLFYEDGS GEKSFVASNDEGVATVGLVSSTGPGGDRVLFVGKGNGPHDNGIIVSTRLLDRTDSREAFEAYTDHATYK AGYLSTNTQQFVAAFEDGPYVFFVFNQQDKHPARNRTLLARMCREDPNYYSYLEMDLQCRDPDIHAAAF GTCLAASVAAPGSGRVLYAVFSRDSRSSGGPGAGLCLFPLDKVHAKMEANRNACYTGTREARDIFYKPF HGDIQCGGHAPGSSKSFPCGSEHLPYPLGSRDGLRGTAVLQRGGLNLTAVTVAAENNHTVAFLGTSDGR ILKVYLTPDGTSSEYDSILVEINKRVKRDLVLSGDLGSLYAMTQDKVFRLPVQECLSYPTCTQCRDSQD PYCGWCVVEGRCTRKAECPRAEEASHWLWSRSKSCVAVTSAQPQNMSRRAQGEVQLTVSPLPALSEEDE LLCLFGESPPHPARVEGEAVICNSPSSIPVTPPGQDHVAVTIQLLLRRGNIFLTSYQYPFYDCRQAMSL EENLPCISCVSNRWTCQWDLRYHECREASPNPEDGIVRAHMEDSCPQFLGPSPLVIPMNHETDVNFQGK NLDTVKGSSLHVGSDLLKFMEPVTMQESGTFAFRTPKLSHDANETLPLHLYVKSYGKNIDSKLHVTLYN CSFGRSDCSLCRAANPDYRCAWCGGQSRCVYEALCNTTSECPPPVITRIQPETGPLGGGIRITILGSNL GVQAGDIQRISVAGRNCSFQPERYSVSTRIVCVIEAAETPFTGGVEVDVFGKLGRSPPNVQFTFQQPKP LSVEPQQGPQAGGTTLTIHGTHLDTGSQEDVRVTLNGVPCKVTKFGAQLQCVTGPQATRGQMLLEVSYG GSPVPNPGIFFTYRENPVLRAFEPLRSFASGGRSINVTGQGFSLIQRFAMVVIAEPLQSWQPPREAESL QPMTVVGTDYVFHNDTKVVFLSPAVPEEPEAYNLTVLIEMDGHRALLRTEAGAFEYVPDPTFENFTGGV KKQVNKLIHARGTNLNKAMTLQEAEAFVGAERCTMKTLTETDLYCEPPEVQPPPKRRQKRDTTHNLPEF IVKFGSREWVLGRVEYDTRVSDVPLSLILPLVIVPMVVVIAVSVYCYWRKSQQAEREYEKIKSQLEGLE ESVRDRCKKEFTDLMIEMEDQTNDVHEAGIPVLDYKTYTDRVFFLPSKDGDKDVMITGKLDIPEPRRPV VEQALYQFSNLLNSKSFLINFIHTLENQREFSARAKVYFASLLTVALHGKLEYYTDIMHTLFLELLEQY VVAKNPKLMLRRSETVVERMLSNWMSICLYQYLKDSAGEPLYKLFKAIKHQVEKGPVDAVQKKAKYTLN DTGLLGDDVEYAPLTVSVIVQDEGVDAIPVKVLNCDTISQVKEKIIDQVYRGQPCSCWPRPDSVVLEWR PGSTAQILSDLDLTSQREGRWKRVNTLMHYNVRDGATLILSKVGVSQQPEDSQQDLPGERHALLEEENR VWHLVRPTDEVDEGKSKRGSVKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLAPGHAVPPAVKY FFDFLDEQAEKHNIQDEDTIHIWKTNSLPLRFWVNILKNPHFIFDVHVHEVVDASLSVIAQTFMDACTR TEHKLSRDSPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTDSLNTLVALHQL YQYTQKYYDEIINALEEDPAAQKMQLAFRLQQIAAALENKVTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012401
ORF Size	5514 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012401.4
RefSeq Size	6369 bp
RefSeq ORF	5517 bp
Locus ID	23654
UniProt ID	O15031
Protein Families	Druggable Genome
Protein Pathways	Axon guidance
MW	205.1 kDa
Gene Summary	Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins (Perrot et al. (2002) [PubMed 12183458]).[supplied by OMIM, Mar 2008]

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