Nesprin 2 (SYNE2) (NM_182910) Human Tagged ORF Clone

CAT#: RC213843

  • TrueORF®

SYNE2 (Myc-DDK-tagged)-Human spectrin repeat containing, nuclear envelope 2 (SYNE2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_182910" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms EDMD5; KASH2; Nesp2; Nesprin-2; NUA; NUANCE; SYNE-2; TROPH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213843 representing NM_182910
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAA
AGCTACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCA
GGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAA
GCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCA
TCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATAT
CCAGGAAATAGAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTA
GTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAGAGCTCCAAA
GTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTT
ACGACAGTCGCTCATGCAGTGCCAGGGCTCTAAGACACGGCCGCGCTCTGATGTGTTGTTTTTTAAGGAC
TTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATG
TCACCGATCCAAAGGCAGACCCCCGGGCTCTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGA
GCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCCTTCTCATTAAGGGACAT
GGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGC
AAGTGTCCCAAGATTTAATGGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGA
GGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCTCCCCGAGCAAAGTTCAGAGCAGTGAGA
ACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCT
CAAGGGTGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCT
GCCCTCCTCCGAAGAAGACTACAGCTGCACTCAGGCCAACAACTTTGCCCGGTCCTTTTACCCCATGCTG
AGGTACACCAATGGGCCACCCCCCACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213843 representing NM_182910
Red=Cloning site Green=Tags(s)

MEGDRNVPPVPPASSTPYKPPYGKLLLPPGTDGGKEGPRVLNGNPQQEDGGLAGITEQQSGAFDRWEMIQ
AQELHNKLKIKQNLQQLNSDISAITTWLKKTEAELEMLKMAKPPSDIQEIELRVKRLQEILKAFDTYKAL
VVSVNVSSKEFLQTESPESTELQSRLRQLSLLWEAAQGAVDSWRGGLRQSLMQCQGSKTRPRSDVLFFKD
FHQLSQNLLLWLASAKNRRQKAHVTDPKADPRALLECRRELMQLEKELVERQPQVDMLQEISNSLLIKGH
GEDCIEAEEKVHVIEKKLKQLREQVSQDLMALQGTQNPASPLPSFDEVDSGDQPPATSVPAPRAKFRAVR
TTEGEEETESRVPGSTRPQRSFLSRVVRAALPLQLLLLLLLLLACLLPSSEEDYSCTQANNFARSFYPML
RYTNGPPPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_182910
ORF Size 1287 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182910.2, NP_878914.1
RefSeq Size 3103 bp
RefSeq ORF 1290 bp
Locus ID 23224
UniProt ID Q8WXH0
Protein Families Druggable Genome, Transcription Factors
MW 48 kDa
Gene Summary The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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