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KDM2B (NM_032590) Human Tagged ORF Clone
CAT＃: RC213999
- TrueORF®
KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1
ORF Plasmid: tGFP
"NM_032590" in other vectors (6)
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CNY 13,712.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CXXC2; Fbl10; FBXL10; JHDM1B; PCCX2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213999 representing NM_032590
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTCCGCAAATGGGGGGATCTGCAGAGGATCACCCCCCACGAAAAAGACATGCAGCAGAAAAGC AAAAAAAGAAAACAGTTATATATACAAAATGCTTTGAATTTGAGTCGGCCACACAGCGCCCGATTGACCG CCAGCGATACGACGAGAACGAGGACTTGTCGGACGTGGAGGAGATCGTCAGCGTCCGCGGCTTCAGCCTG GAGGAGAAGCTTCGCAGCCAGCTGTACCAGGGGGACTTCGTGCACGCCATGGAGGGCAAAGATTTCAACT ATGAGTACGTACAGAGAGAAGCTCTCAGGGTTCCCCTGATATTTCGAGAAAAGGATGGACTGGGAATTAA GATGCCTGACCCTGATTTCACAGTCCGAGACGTCAAACTCCTAGTGGGGAGCCGGCGGCTTGTGGACGTG ATGGATGTGAACACCCAGAAGGGCACGGAGATGAGCATGTCCCAGTTTGTGCGTTACTACGAGACGCCCG AGGCCCAGCGGGACAAGCTGTACAACGTCATCAGCCTAGAGTTCAGCCACACCAAGCTGGAGCACTTGGT CAAGCGTCCGACTGTGGTAGACCTGGTGGACTGGGTGGACAACATGTGGCCCCAGCATCTGAAGGAGAAG CAGACAGAAGCCACGAACGCCATTGCAGAGATGAAGTACCCGAAAGTGAAAAAGTACTGTCTGATGAGCG TGAAAGGTTGTTTCACCGACTTCCACATCGACTTTGGAGGCACTTCCGTTTGGTACCATGTTTTCCGGGG TGGGAAGATTTTTTGGCTGATTCCTCCAACGCTGCACAATTTGGCGCTGTACGAGGAGTGGGTGCTGTCA GGCAAACAGAGTGACATCTTTCTGGGAGACCGTGTGGAACGATGCCAAAGAATTGAGCTGAAGCAGGGCT ACACATTTTTCATCCCTTCCGGTTGGATCCATGCCGTCTACACCCCTGTAGACTCTTTGGTGTTCGGCGG AAACATCCTGCACAGCTTTAACGTGCCCATGCAGCTGCGGATCTACGAGATCGAGGACAGGACGCGGGTG CAGCCCAAATTCCGTTACCCCTTCTACTATGAGATGTGCTGGTATGTCCTGGAGAGATACGTGTACTGTG TGACCCAGCGCTCCCACCTCACTCAGGAATACCAGAGGGAGTCGATGCTTATTGATGCCCCGAGGAAGCC CAGCATAGACGGCTTCTCTTCGGATTCCTGGCTGGAGATGGAGGAGGAGGCCTGTGATCAGCAGCCTCAG GAGGAGGAGGAGAAGGACGAGGAGGGCGAGGGCAGGGACAGGGCACCCAAACCGCCCACCGATGGCTCCA CTTCACCCACCAGCACGCCCTCTGAGGACCAGGAGGCCCTCGGGAAGAAGCCCAAAGCACCTGCCCTGCG ATTCCTCAAAAGGACTTTGTCTAATGAGTCGGAGGAAAGTGTGAAGTCCACCACATTGGCCGTAGACTAC CCCAAGACCCCCACCGGCTCTCCCGCCACGGAGGTCTCTGCCAAATGGACCCATCTCACTGAGTTTGAAC TGAAGGGCCTGAAAGCTCTGGTGGAGAAACTGGAATCCCTCCCGGAGAACAAGAAGTGTGTCCCCGAGGG CATCGAGGACCCCCAGGCACTCCTGGAGGGTGTGAAGAACGTCCTGAAGGAGCACGCAGATGATGACCCT AGTCTGGCCATCACTGGGGTCCCTGTGGTGACTTGGCCAAAGAAGACTCCAAAGAACCGGGCTGTGGGTC GGCCCAAGGGGAAGCTGGGCCCGGCCTCCGCGGTGAAGTTGGCCGCCAACCGGACAACGGCAGGAGCTCG GCGGCGCCGGACGCGATGCCGCAAGTGCGAGGCCTGCCTGCGGACCGAGTGCGGAGAGTGCCACTTCTGC AAGGACATGAAGAAGTTCGGGGGCCCCGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCGCGC CAGTGCTGCCCCACACCGCCGTGTGCCTTGTGTGTGGCGAGGCGGGGAAGGAAGACACGGTGGAAGAGGA GGAAGGCAAGTTTAACCTCATGCTCATGGAGTGCTCCATCTGCAATGAAATCATCCACCCTGGATGCCTT AAGATTAAGGAGTCAGAGGGTGTGGTCAACGACGAGCTTCCAAACTGCTGGGAGTGTCCGAAGTGTAACC ACGCCGGCAAGACCGGGAAACAAAAGCGTGGCCCTGGCTTTAAGTACGCCTCCAACCTGCCCGGCTCCCT GCTCAAGGAGCAGAAGATGAACCGGGACAACAAGGAAGGGCAGGAACCTGCCAAGCGGAGGAGTGAGTGT GAGGAGGCGCCCCGGCGCAGGTCGGATGAGCACTCGAAGAAGGTGCCGCCGGACGGCCTTCTGCGCAGAA AGTCTGACGACGTGCACCTGAGGAAGAAGCGGAAATACGAGAAGCCCCAGGAGCTGAGTGGACGCAAGCG GGCCTCATCGCTTCAAACGTCCCCCGGTTCCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGCAGC CTCAGCCCCTGGTGGAGATCCAGTCTCACTTACTTCCAGCAGCAGCTCAAACCTGGCAAAGAAGATAAGC TTTTCAGGAAAAAGCGGCGGTCCTGGAAGAACGCCGAGGACCGCATGGCGCTGGCCAACAAGCCCCTCCG GCGCTTCAAGCAGGAACCCGAGGACGAACTGCCCGAGGCGCCCCCCAAGACCAGGGAGAGCGACCACTCC CGCTCCAGCTCCCCCACCGCGGGACCCAGCACCGAAGGGGCCGAGGGCCCGGAGGAGAAGAAGAAGGTGA AGATGCGCCGGAAGCGGCGGCTTCCCAACAAGGAGCTGAGCAGGGAGCTGAGCAAGGAGCTCAACCACGA GATCCAGAGGACGGAGAACAGCCTGGCCAACGAGAACCAGCAGCCCATCAAGTCGGAGCCTGAGAGCGAG GGCGAGGAGCCCAAGCGGCCCCCGGGCATCTGCGAGCGTCCCCACCGCTTCAGCAAGGGGCTCAACGGCA CCCCCCGGGAGCTGCGGCACCAGCTGGGGCCCAGCCTGCGCAGCCCGCCCCGTGTCATCTCCCGGCCCCC ACCCTCCGTGTCCCCGCCCAAGTGTATCCAGATGGAGCGCCATGTGATCCGGCCACCCCCCATCAGCCCC CCGCCTGACTCGCTACCCCTGGACGATGGGGCAGCCCACGTCATGCACAGGGAGGTGTGGATGGCCGTCT TCAGCTACCTCAGCCACCAAGACCTGTGTGTGTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTGCTG CGATAAGCGGTTGTGGACCCGCATTGACCTGAACCACTGCAAGTCTATCACACCCCTGATGCTGAGTGGC ATCATCCGGCGACAGCCCGTCTCCCTCGACCTCAGCTGGACCAATATCTCCAAGAAGCAGCTGAGCTGGC TCATCAACCGGCTGCCTGGGCTCCGGGACTTGGTGCTGTCAGGCTGCTCATGGATCGCGGTCTCGGCCCT TTGCAGCTCCAGTTGTCCGCTGCTCCGGACCCTGGATGTCCAGTGGGTGGAGGGACTAAAGGATGCCCAG ATGCGGGATCTCCTGTCCCCGCCCACAGACAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCCGGA ACATCGTGGAGCTGCGCCTGGCAGGCCTGGACATCACAGATGCCTCCCTGCGGCTCATCATCCGCCACAT GCCCCTGCTCTCCAAGCTCCACCTCAGTTACTGTAACCACGTCACCGACCAGTCTATCAACCTGCTCACT GCTGTTGGCACCACCACCCGAGACTCCTTAACCGAGATCAACCTGTCTGACTGCAATAAGGTCACTGATC AGTGCCTGTCCTTCTTCAAACGCTGTGGAAACATCTGTCATATTGACCTGAGGTACTGCAAGCAAGTCAC CAAGGAAGGCTGTGAGCAGTTCATAGCCGAGATGTCTGTGAGTGTCCAGTTTGGGCAAGTAGAAGAAAAA CTCCTGCAAAAACTGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213999 representing NM_032590
Red=Cloning site Green=Tags(s) MAGPQMGGSAEDHPPRKRHAAEKQKKKTVIYTKCFEFESATQRPIDRQRYDENEDLSDVEEIVSVRGFSL EEKLRSQLYQGDFVHAMEGKDFNYEYVQREALRVPLIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDV MDVNTQKGTEMSMSQFVRYYETPEAQRDKLYNVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEK QTEATNAIAEMKYPKVKKYCLMSVKGCFTDFHIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLS GKQSDIFLGDRVERCQRIELKQGYTFFIPSGWIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRV QPKFRYPFYYEMCWYVLERYVYCVTQRSHLTQEYQRESMLIDAPRKPSIDGFSSDSWLEMEEEACDQQPQ EEEEKDEEGEGRDRAPKPPTDGSTSPTSTPSEDQEALGKKPKAPALRFLKRTLSNESEESVKSTTLAVDY PKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHADDDP SLAITGVPVVTWPKKTPKNRAVGRPKGKLGPASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFC KDMKKFGGPGRMKQSCIMRQCIAPVLPHTAVCLVCGEAGKEDTVEEEEGKFNLMLMECSICNEIIHPGCL KIKESEGVVNDELPNCWECPKCNHAGKTGKQKRGPGFKYASNLPGSLLKEQKMNRDNKEGQEPAKRRSEC EEAPRRRSDEHSKKVPPDGLLRRKSDDVHLRKKRKYEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSS LSPWWRSSLTYFQQQLKPGKEDKLFRKKRRSWKNAEDRMALANKPLRRFKQEPEDELPEAPPKTRESDHS RSSSPTAGPSTEGAEGPEEKKKVKMRRKRRLPNKELSRELSKELNHEIQRTENSLANENQQPIKSEPESE GEEPKRPPGICERPHRFSKGLNGTPRELRHQLGPSLRSPPRVISRPPPSVSPPKCIQMERHVIRPPPISP PPDSLPLDDGAAHVMHREVWMAVFSYLSHQDLCVCMRVCRTWNRWCCDKRLWTRIDLNHCKSITPLMLSG IIRRQPVSLDLSWTNISKKQLSWLINRLPGLRDLVLSGCSWIAVSALCSSSCPLLRTLDVQWVEGLKDAQ MRDLLSPPTDNRPGQMDNRSKLRNIVELRLAGLDITDASLRLIIRHMPLLSKLHLSYCNHVTDQSINLLT AVGTTTRDSLTEINLSDCNKVTDQCLSFFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEK LLQKLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032590 |
| ORF Size | 4008 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032590.5 |
| RefSeq Size | 5287 bp |
| RefSeq ORF | 4011 bp |
| Locus ID | 84678 |
| UniProt ID | Q8NHM5 |
| Protein Families | Druggable Genome |
| MW | 152.4 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213999L1 | Lenti-ORF clone of KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1 |
CNY 16,112.00 |
|
| RC213999L2 | Lenti-ORF clone of KDM2B (mGFP-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1 |
CNY 12,730.00 |
|
| RC213999L3 | Lenti-ORF clone of KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1 |
CNY 16,112.00 |
|
| RC213999L4 | Lenti-ORF clone of KDM2B (mGFP-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1 |
CNY 16,112.00 |
|
| RG213999 | KDM2B (tGFP-tagged) - Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1 |
CNY 15,312.00 |
|
| SC305484 | KDM2B (untagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1 |
CNY 13,720.00 |
