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Artemis (DCLRE1C) (NM_001033855) Human Tagged ORF Clone
CAT＃: RC214185
DCLRE1C (Myc-DDK-tagged)-Human DNA cross-link repair 1C (DCLRE1C), transcript variant a
ORF Plasmid: tGFP
"NM_001033855" in other vectors (4)
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CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A-SCID; DCLREC1C; RS-SCID; SCIDA; SNM1C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214185 representing NM_001033855
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTTTCGAGGGGCAGATGGCCGAGTATCCAACTATCTCCATAGACCGCTTCGATAGGGAGAACC TGAGGGCCCGCGCCTACTTCCTGTCCCACTGCCACAAAGATCACATGAAAGGATTAAGAGCCCCTACCTT GAAAAGAAGGTTGGAGTGCAGCTTGAAGGTTTATCTATACTGTTCACCTGTGACTAAGGAGTTGTTGTTA ACGAGCCCGAAATACAGATTTTGGAAGAAACGAATTATATCTATTGAAATCGAGACTCCTACCCAGATAT CTTTAGTGGATGAAGCATCAGGAGAGAAGGAAGAGATTGTTGTGACTCTCTTACCAGCTGGTCACTGTCC GGGATCAGTTATGTTTTTATTTCAGGGCAATAATGGAACTGTCCTGTACACAGGAGACTTCAGATTGGCG CAAGGAGAAGCTGCTAGAATGGAGCTTCTGCACTCCGGGGGCAGAGTCAAAGACATCCAAAGTGTATATT TGGATACTACGTTCTGTGATCCAAGATTTTACCAAATTCCAAGTCGGGAGGAGTGTTTAAGTGGAGTCTT AGAGCTGGTCCGAAGCTGGATCACTCGGAGCCCGTACCATGTTGTGTGGCTGAACTGCAAAGCGGCTTAT GGCTATGAATATCTGTTCACCAACCTTAGTGAAGAATTAGGAGTCCAGGTTCATGTGAATAAGCTAGACA TGTTTAGGAACATGCCTGAGATCCTTCATCATCTCACAACAGACCGCAACACTCAGATCCATGCATGCCG GCATCCCAAGGCAGAGGAATATTTTCAGTGGAGCAAATTACCCTGTGGAATTACTTCCAGAAATAGAATT CCACTCCACATAATCAGCATTAAGCCATCCACCATGTGGTTTGGAGAAAGGAGCAGAAAAACAAATGTAA TTGTGAGGACTGGAGAGAGTTCATACAGAGCTTGTTTTTCTTTTCACTCCTCCTACAGTGAGATTAAAGA TTTCTTGAGCTACCTCTGTCCTGTGAACGCATATCCAAATGTCATTCCAGTTGGCACAACTATGGATAAA GTTGTCGAAATCTTAAAGCCTTTATGCCGGTCTTCCCAAAGTACGGAGCCAAAGTATAAACCACTGGGAA AACTGAAGAGAGCTAGAACAGTTCACCGAGACTCAGAGGAGGAAGATGACTATCTCTTTGATGATCCTCT GCCAATACCTTTAAGGCACAAAGTTCCATACCCGGAAACTTTTCACCCTGAGGTATTTTCAATGACTGCA GTATCAGAAAAGCAGCCTGAAAAACTGAGACAAACCCCAGGATGCTGCAGAGCAGAGTGTATGCAGAGCT CTCGTTTCACAAACTTTGTAGATTGTGAAGAATCCAACAGTGAAAGTGAAGAAGAAGTAGGAATCCCAGC TTCACTGCAAGGAGATCTGGGCTCTGTACTTCACCTGCAAAAGGCTGATGGGGATGTACCCCAGTGGGAA GTATTCTTTAAAAGAAATGATGAAATCACAGATGAGAGTTTGGAAAACTTCCCTTCCTCCACAGTGGCAG GGGGATCTCAGTCACCAAAGCTTTTCAGTGACTCTGATGGAGAATCAACTCACATCTCCTCCCAGAATTC TTCCCAGTCAACACACATAACAGAACAAGGAAGTCAAGGCTGGGACAGCCAATCTGATACTGTTTTGTTA TCTTCCCAAGAGAGAAACAGTGGGGATATTACTTCCTTGGACAAAGCTGACTACAGACCAACAATCAAAG AGAATATTCCTGCCTCTCTCATGGAACAAAATGTAATTTGCCCAAAGGATACTTACTCTGATTTGAAAAG CAGAGATAAAGATGTGACAATAGTTCCTAGTACTGGAGAACCAACTACTCTAAGCAGTGAGACACATATA CCCGAGGAAAAAAGTTTGCTAAATCTTAGCACAAATGCAGATTCCCAGAGCTCTTCTGATTTTGAAGTTC CCTCAACTCCAGAAGCTGAGTTACCTAAACGAGAGCATTTACAATATTTATATGAGAAGCTGGCAACTGG TGAGAGTATAGCAGTCAAAAAAAGAAAATGCTCACTCTTAGATACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214185 representing NM_001033855
Red=Cloning site Green=Tags(s) MSSFEGQMAEYPTISIDRFDRENLRARAYFLSHCHKDHMKGLRAPTLKRRLECSLKVYLYCSPVTKELLL TSPKYRFWKKRIISIEIETPTQISLVDEASGEKEEIVVTLLPAGHCPGSVMFLFQGNNGTVLYTGDFRLA QGEAARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREECLSGVLELVRSWITRSPYHVVWLNCKAAY GYEYLFTNLSEELGVQVHVNKLDMFRNMPEILHHLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSRNRI PLHIISIKPSTMWFGERSRKTNVIVRTGESSYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIPVGTTMDK VVEILKPLCRSSQSTEPKYKPLGKLKRARTVHRDSEEEDDYLFDDPLPIPLRHKVPYPETFHPEVFSMTA VSEKQPEKLRQTPGCCRAECMQSSRFTNFVDCEESNSESEEEVGIPASLQGDLGSVLHLQKADGDVPQWE VFFKRNDEITDESLENFPSSTVAGGSQSPKLFSDSDGESTHISSQNSSQSTHITEQGSQGWDSQSDTVLL SSQERNSGDITSLDKADYRPTIKENIPASLMEQNVICPKDTYSDLKSRDKDVTIVPSTGEPTTLSSETHI PEEKSLLNLSTNADSQSSSDFEVPSTPEAELPKREHLQYLYEKLATGESIAVKKRKCSLLDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033855 |
| ORF Size | 2076 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001033855.3 |
| RefSeq Size | 3701 bp |
| RefSeq ORF | 2079 bp |
| Locus ID | 64421 |
| UniProt ID | Q96SD1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Non-homologous end-joining, Primary immunodeficiency |
| MW | 78.3 kDa |
| Gene Summary | This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The encoded protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Mutations in this gene can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214185L3 | Lenti-ORF clone of DCLRE1C (Myc-DDK-tagged)-Human DNA cross-link repair 1C (DCLRE1C), transcript variant a |
CNY 9,220.00 |
|
| RC214185L4 | Lenti-ORF clone of DCLRE1C (mGFP-tagged)-Human DNA cross-link repair 1C (DCLRE1C), transcript variant a |
CNY 9,220.00 |
|
| RG214185 | DCLRE1C (tGFP-tagged) - Human DNA cross-link repair 1C (DCLRE1C), transcript variant a |
CNY 8,080.00 |
|
| SC302772 | DCLRE1C (untagged)-Human DNA cross-link repair 1C (DCLRE1C), transcript variant a |
CNY 7,616.00 |
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