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GCKR (NM_001486) Human Tagged ORF Clone
CAT＃: RC214230
GCKR (Myc-DDK-tagged)-Human glucokinase (hexokinase 4) regulator (GCKR)
ORF Plasmid: tGFP
"NM_001486" in other vectors (6)
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CNY 6,976.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FGQTL5; GKRP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214230 representing NM_001486
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGCACAAAACGGTTTCAACATGTCATTGAGACCCCGGAGCCTGGCAAGTGGGAGTTGTCTGGGT ACGAGGCAGCTGTGCCAATCACGGAGAAGTCAAACCCACTGACCCAGGATCTAGACAAAGCAGATGCTGA GAACATTGTTCGACTGCTAGGGCAATGTGATGCTGAGATCTTCCAGGAGGAGGGGCAAGCCCTGTCCACA TACCAGAGACTCTACAGCGGATCCATTCTGACCACCATGGTACAGGTGGCTGGGAAAGTTCAGGAAGTGC TGAAGGAGCCAGATGGGGGGCTGGTTGTGCTGAGTGGAGGGGGCACCTCTGGCCGGATGGCATTCCTCAT GTCGGTGTCCTTTAATCAGCTGATGAAAGGTCTGGGACAGAAACCTCTTTACACCTACCTCATTGCAGGT GGTGACAGGTCTGTGGTGGCCTCTAGGGAGGGGACAGAAGATAGTGCCTTGCACGGGATTGAGGAACTGA AGAAGGTGGCTGCCGGGAAGAAGAGAGTGATTGTCATTGGCATTTCTGTGGGACTCTCTGCTCCCTTTGT GGCAGGCCAGATGGACTGCTGCATGAACAACACAGCTGTCTTCTTGCCAGTCCTGGTTGGCTTCAATCCA GTGAGCATGGCCAGAAATGACCCCATTGAAGACTGGAGTTCAACATTCCGACAAGTAGCAGAGCGGATGC AGAAAATGCAGGAGAAACAGAAAGCTTTTGTGCTCAATCCTGCCATCGGGCCCGAGGGTCTCAGCGGCTC CTCCCGGATGAAAGGTGGAAGTGCCACCAAGATTCTGCTGGAAACCCTGTTATTAGCAGCCCATAAGACT GTGGACCAGGGCATTGCAGCATCTCAAAGATGCCTCCTGGAAATCTTGCGGACATTTGAGCGAGCTCATC AGGTGACCTACAGCCAAAGCCCCAAGATTGCCACCCTGATGAAGAGTGTCAGCACCAGTCTGGAGAAGAA AGGCCACGTGTACCTGGTTGGCTGGCAGACCCTGGGCATCATTGCCATCATGGATGGAGTAGAGTGCATC CACACCTTTGGTGCTGATTTCCGAGATGTCCGTGGCTTTCTCATTGGTGATCACAGTGACATGTTTAACC AGAAGGCTGAGCTCACCAACCAGGGTCCCCAGTTCACCTTCTCCCAGGAGGACTTCCTGACTTCCATCCT TCCCTCTCTCACGGAAATCGATACTGTGGTCTTCATTTTCACCCTGGATGACAACCTCACGGAGGTGCAG ACTATAGTGGAGCAGGTGAAAGAGAAGACCAACCACATCCAGGCCCTGGCACACAGCACCGTGGGTCAGA CCTTGCCGATCCCTCTGAAGAAGCTCTTTCCCTCCATCATCAGCATCACATGGCCACTGCTTTTCTTTGA ATATGAAGGGAACTTCATCCAGAAGTTCCAGCGTGAGCTAAGCACCAAATGGGTGCTGAATACAGTGAGT ACAGGTGCTCATGTGCTTCTTGGTAAGATCCTACAAAACCACATGTTGGACCTTCGGATTAGCAACTCCA AGCTCTTCTGGCGGGCGCTGGCCATGCTGCAGCGGTTCTCTGGACAGTCCAAGGCTCGATGCATCGAGAG CCTCCTCCGAGCGATCCACTTTCCCCAGCCACTGTCAGATGATATTCGGGCTGCTCCCATCTCCTGCCAT GTCCAGGTTGCACATGAGAAGGAACAGGTGATACCCATCGCCTTGCTGAGCCTCCTATTCCGGTGCTCGA TCACTGAGGCTCAGGCACACCTGGCTGCAGCTCCTTCTGTCTGTGAGGCTGTCAGGAGTGCTCTTGCTGG GCCAGGTCAGAAGCGCACTGCGGACCCCCTCGAGATCCTAGAGCCTGACGTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214230 representing NM_001486
Red=Cloning site Green=Tags(s) MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALST YQRLYSGSILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAG GDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNP VSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKT VDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECI HTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQ TIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVS TGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCH VQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001486 |
| ORF Size | 1875 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001486.4 |
| RefSeq Size | 2197 bp |
| RefSeq ORF | 1878 bp |
| Locus ID | 2646 |
| UniProt ID | Q14397 |
| Domains | SIS |
| MW | 69.1 kDa |
| Gene Summary | This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214230L1 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), Myc-DDK-tagged |
CNY 9,376.00 |
|
| RC214230L2 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), mGFP tagged |
CNY 5,890.00 |
|
| RC214230L3 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214230L4 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), mGFP tagged |
CNY 5,890.00 |
|
| RG214230 | GCKR (tGFP-tagged) - Human glucokinase (hexokinase 4) regulator (GCKR) |
CNY 8,576.00 |
|
| SC119244 | GCKR (untagged)-Human glucokinase (hexokinase 4) regulator (GCKR) |
CNY 6,992.00 |
