TLL2 (NM_012465) Human Tagged ORF Clone

CAT#: RC214366

TLL2 (Myc-DDK-tagged)-Human tolloid-like 2 (TLL2)

ORF Plasmid: DDK tGFP



  "NM_012465" in other vectors (6)

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CNY 7,072.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TLL2 Rabbit Polyclonal (CUB 2 Domain) Antibody
    • 50 ug

CNY 5,654.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214366 representing NM_012465
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCGGGCGACTGCACTTGGGGCCCTGGTGTCACTGCTGCTGCTGCTGCCGCTGCCTCGCGGCGCCG
GGGGACTCGGGGAGCGCCCGGACGCCACCGCAGACTACTCAGAGCTGGACGGCGAGGAGGGCACGGAGCA
GCAGCTGGAGCATTACCACGACCCTTGCAAAGCCGCTGTCTTTTGGGGAGACATTGCCTTAGATGAAGAT
GACTTGAAGCTGTTTCACATTGACAAAGCCAGAGACTGGACCAAGCAGACAGTGGGGGCAACAGGACACA
GCACAGGTGGGCTTGAAGAGCAGGCATCTGAGAGCAGCCCAGACACCACAGCCATGGACACTGGCACCAA
GGAAGCTGGAAAGGATGGCCGGGAGAATACCACACTCCTGCACAGCCCTGGGACCTTGCATGCCGCAGCC
AAGACCTTCTCTCCCCGGGTCCGAAGAGCCACAACCTCAAGGACAGAGAGGATATGGCCTGGAGGAGTCA
TCCCCTACGTCATTGGAGGGAACTTCACTGGGAGCCAGAGGGCCATTTTTAAGCAGGCCATGAGACACTG
GGAGAAGCACACCTGTGTGACCTTCATAGAAAGGACGGATGAGGAAAGCTTTATTGTATTCAGTTACAGA
ACCTGTGGCTGTTGCTCCTATGTTGGGCGCCGAGGAGGAGGCCCACAGGCCATATCCATTGGGAAGAACT
GTGACAAGTTTGGCATTGTGGCTCACGAGCTGGGCCATGTGGTTGGGTTTTGGCATGAACACACCCGGCC
AGACAGAGACCAACATGTCACCATCATCAGGGAAAACATCCAGCCAGGTCAGGAGTATAATTTCTTAAAA
ATGGAAGCTGGGGAAGTGAGCTCTCTGGGAGAGACATACGACTTTGACAGCATCATGCACTACGCCCGGA
ACACCTTCTCAAGAGGAGTTTTCTTAGACACCATCCTTCCCCGTCAAGATGACAATGGCGTCAGGCCAAC
CATTGGCCAGCGCGTGCGGCTCAGTCAGGGAGACATAGCTCAAGCCCGGAAGCTGTACAAATGCCCAGCG
TGTGGGGAGACCCTGCAGGACACAACGGGAAACTTTTCTGCACCTGGTTTCCCAAATGGGTACCCATCTT
ACTCCCACTGCGTCTGGAGGATCTCGGTCACCCCAGGGGAAAAGATCGTATTAAACTTCACATCCATGGA
TTTGTTTAAAAGCCGACTGTGCTGGTATGATTACGTGGAGGTCCGGGATGGTTACTGGAGAAAAGCCCCC
CTTTTGGGCAGGTTTTGTGGCGATAAGATCCCGGAGCCCCTCGTCTCCACGGACAGCCGGCTCTGGGTGG
AGTTCCGCAGCAGCAGCAACATCTTGGGCAAGGGCTTCTTTGCAGCGTACGAAGCTACCTGCGGGGGAGA
CATGAACAAAGATGCCGGTCAGATTCAATCTCCCAACTATCCGGATGACTACAGACCTTCCAAGGAATGT
GTCTGGAGGATTACGGTTTCAGAGGGGTTTCACGTGGGACTTACCTTCCAAGCTTTTGAGATTGAAAGGC
ACGACAGCTGTGCATATGACTACCTGGAAGTCCGGGATGGCCCCACGGAAGAGAGTGCCCTGATCGGCCA
CTTTTGTGGCTATGAGAAGCCGGAGGATGTGAAATCGAGCTCCAACAGACTGTGGATGAAGTTTGTGTCC
GATGGCTCTATCAATAAAGCGGGCTTTGCAGCCAATTTTTTCAAGGAGGTGGATGAGTGTTCCTGGCCAG
ATCACGGCGGGTGCGAGCATCGCTGTGTGAACACGCTGGGCAGCTACAAGTGTGCCTGTGACCCTGGCTA
CGAGCTGGCCGCCGATAAGAAGATGTGTGAAGTGGCCTGTGGCGGTTTCATTACCAAGCTGAATGGAACC
ATCACCAGCCCTGGGTGGCCGAAGGAGTATCCCACAAACAAAAACTGTGTCTGGCAGGTGGTGGCCCCCG
CTCAGTACCGGATCTCCCTTCAGTTTGAAGTGTTTGAACTGGAAGGCAATGACGTCTGTAAGTACGACTT
TGTAGAGGTGCGCAGCGGCCTGTCCCCCGACGCCAAGCTGCACGGCAGGTTCTGCGGCTCTGAGACGCCG
GAGGTCATCACCTCGCAGAGCAACAACATGCGCGTGGAGTTCAAGTCCGACAACACCGTCTCCAAGCGCG
GCTTCAGGGCCCACTTCTTCTCAGATAAGGACGAGTGTGCCAAGGACAACGGCGGGTGTCAGCATGAGTG
CGTCAACACCTTCGGGAGCTACCTGTGCAGGTGCAGAAACGGCTACTGGCTCCACGAGAATGGGCATGAC
TGCAAAGAGGCTGGCTGTGCACACAAGATCAGCAGTGTGGAGGGGACCCTGGCGAGCCCCAACTGGCCTG
ACAAATACCCCAGCCGGAGGGAGTGTACCTGGAACATCTCTTCGACTGCAGGCCACAGAGTGAAACTCAC
CTTTAATGAGTTTGAGATCGAGCAGCACCAGGAATGTGCCTATGACCACCTGGAAATGTATGACGGGCCG
GACAGCCTGGCCCCCATTCTGGGCCGTTTCTGCGGCAGCAAGAAACCAGACCCCACGGTGGCTTCCGGCA
GCAGTATGTTTCTCAGGTTTTATTCGGATGCCTCAGTGCAGAGGAAAGGCTTCCAGGCAGTGCACAGCAC
AGAGTGCGGGGGCAGGCTGAAGGCTGAAGTGCAGACCAAAGAGCTCTATTCCCACGCCCAGTTTGGGGAC
AACAACTACCCGAGCGAGGCCCGCTGTGACTGGGTGATCGTGGCAGAGGACGGCTACGGCGTGGAGCTGA
CATTCCGGACCTTTGAGGTTGAGGAGGAGGCCGACTGCGGCTACGACTACATGGAAGCCTACGACGGCTA
CGACAGCTCAGCGCCCAGGCTCGGCCGCTTCTGTGGCTCTGGGCCATTAGAAGAAATCTACTCTGCAGGT
GATTCCCTGATGATTCGATTCCGCACAGATGACACCATCAACAAGAAAGGCTTTCATGCCCGATACACCA
GCACCAAGTTCCAGGATGCCCTGCACATGAAGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214366 representing NM_012465
Red=Cloning site Green=Tags(s)

MPRATALGALVSLLLLLPLPRGAGGLGERPDATADYSELDGEEGTEQQLEHYHDPCKAAVFWGDIALDED
DLKLFHIDKARDWTKQTVGATGHSTGGLEEQASESSPDTTAMDTGTKEAGKDGRENTTLLHSPGTLHAAA
KTFSPRVRRATTSRTERIWPGGVIPYVIGGNFTGSQRAIFKQAMRHWEKHTCVTFIERTDEESFIVFSYR
TCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLK
MEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKCPA
CGETLQDTTGNFSAPGFPNGYPSYSHCVWRISVTPGEKIVLNFTSMDLFKSRLCWYDYVEVRDGYWRKAP
LLGRFCGDKIPEPLVSTDSRLWVEFRSSSNILGKGFFAAYEATCGGDMNKDAGQIQSPNYPDDYRPSKEC
VWRITVSEGFHVGLTFQAFEIERHDSCAYDYLEVRDGPTEESALIGHFCGYEKPEDVKSSSNRLWMKFVS
DGSINKAGFAANFFKEVDECSWPDHGGCEHRCVNTLGSYKCACDPGYELAADKKMCEVACGGFITKLNGT
ITSPGWPKEYPTNKNCVWQVVAPAQYRISLQFEVFELEGNDVCKYDFVEVRSGLSPDAKLHGRFCGSETP
EVITSQSNNMRVEFKSDNTVSKRGFRAHFFSDKDECAKDNGGCQHECVNTFGSYLCRCRNGYWLHENGHD
CKEAGCAHKISSVEGTLASPNWPDKYPSRRECTWNISSTAGHRVKLTFNEFEIEQHQECAYDHLEMYDGP
DSLAPILGRFCGSKKPDPTVASGSSMFLRFYSDASVQRKGFQAVHSTECGGRLKAEVQTKELYSHAQFGD
NNYPSEARCDWVIVAEDGYGVELTFRTFEVEEEADCGYDYMEAYDGYDSSAPRLGRFCGSGPLEEIYSAG
DSLMIRFRTDDTINKKGFHARYTSTKFQDALHMKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012465
ORF Size 3045 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012465.4
RefSeq Size 6753 bp
RefSeq ORF 3048 bp
Locus ID 7093
UniProt ID Q9Y6L7
Domains CUB, Astacin, EGF_CA, ZnMc, EGF, EGF
Protein Families Druggable Genome, Secreted Protein
MW 113.4 kDa
Gene Summary This gene encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. Unlike other family members, a similar protein in mice does not cleave procollagen C-propeptides or chordin. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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