FAM123B (AMER1) (NM_152424) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FAM123B; OSCS; WTX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC214530 representing NM_152424 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCCAAAAGGATGAAGCTGCTCAGGCCAAGGGAGCTGCAGCCTCTGGGAGTACCCGTGAACAAA CAGCAGAAAAAGGAGCCAAGAACAAGGCAGCTGAGGCGACAGAAGGACCAACCTCAGAGCCATCCTCATC CGGCCCAGGTAGGCTGAAGAAAACTGCCATGAAACTCTTTGGTGGCAAGAAGGGTATCTGTACTCTGCCT AGTTTCTTTGGAGGGGGACGGAGCAAAGGTTCTGGGAAAGGCAGCTCCAAGAAAGGTCTCAGCAAGAGCA AGACCCACGATGGCCTGAGTGAAGCAGCCCATGGCCCTGAAGATGTTGTCAGTGAAGGAACTGGCTTCTC CCTGCCTTTGCCTGAGTTACCCTGCCAATTTCCCAGCTCTCAGAGTGCCCATGGGGCTTTGGAGACAGGC TCCAGATGTAAGACATCTGTGGCTGGAGCCACAGAGAAAGCTGTGGCTGAGAAGTTTCCCTCTATGCCCA AGCCAAAGAAAGGCCTAAAAGGCTTTTTTAGCAGTATCCGCCGTCACCGGAAGAGCAAGGTCACTGGGGC TGAGCAAAGTGAGCCAGGGGCCAAGGGGCCTGAGAGGGTCAGAGCCAGGCCTCATGAGCACGTGAGCTCA GCCCCTCAGGTGCCCTGCTTTGAGGAGACCTTCCAAGCCCCTAGAAAGGAAAATGCTAACCCCCAAGATG CCCCTGGGCCAAAAGTTTCTCCAACACCAGAACCTTCTCCACCAGCTACTGAGAAAATGGCCTGTAAAGA TCCAGAAAAACCCATGGAGGCCTGTGCCTCAGCACATGTGCAACCCAAGCCTGCCCCTGAAGCCAGTAGC CTAGAGGAGCCCCATAGCCCAGAAACAGGGGAGAAGGTAGTAGCAGGAGAGGTAAACCCACCCAATGGCC CTGTGGGGGACCCACTGAGCCTCTTGTTTGGGGATGTGACATCCCTGAAAAGCTTTGATTCATTGACAGG TTGTGGTGACATAATAGCAGAACAGGACATGGACAGTATGACAGACAGCATGGCCTCTGGGGGCCAGAGA GCAAACCGAGATGGGACCAAGCGAAGTTCCTGCCTGGTGACCTACCAAGGAGGTGGGGAGGAGATGGCCT TGCCAGATGATGATGACGAGGAGGAAGAAGAGGAAGAAGAGGTGGAATTAGAGGAGGAAGAAGAGGAGGT TAAGGAGGAGGAAGAAGATGATGACTTAGAATATCTGTGGGAAACTGCCCAAATGTATCCACGGCCCAAT ATGAACCTGGGCTACCATCCCACCACATCCCCAGGCCACCACGGCTACATGCTCCTTGACCCAGTTAGGT CTTATCCTGGCCTAGCCCCTGGGGAACTTTTGACTCCTCAGAGTGACCAGCAAGAATCCGCCCCCAATAG TGATGAAGGTTATTATGACTCCACCACACCTGGATTTGAGGATGATTCAGGTGAGGCCCTGGGGCTTGTC CGCAGGGATTGTCTACCCCGAGACAGCTACAGTGGAGATGCCCTATATGAGTTCTATGAGCCAGATGACA GCCTTGAGAACTCTCCACCTGGAGATGACTGCCTTTATGACCTCCATGGTCGAAGCTCTGAGATGTTTGA CCCCTTCTTAAACTTTGAGCCCTTTTTGTCCTCCCGGCCACCTGGGGCAATGGAGACAGAGGAAGAACGG CTAGTGACCATCCAGAAACAGTTGTTGTATTGGGAGCTTCGGCGGGAGCAGCTTGAGGCCCAGGAGGCAC GTGCCCGAGAAGCTCATGCCAGGGAGGCCCACGCCAGGGAGGCCTATACTCGAGAGGCTTATGGCAGGGA AGCCTATGCCAGGGAGGCCCACACTTGGGAAGCTCATGGCAGGGAGGCCAGAACCCGAGAAGCCCAGGCC CGAGAGGTTCGTTGTAGAGAGACTCAAGTCCGAGAGACCCAGGCCCGGCAGGAGAAGCCCGTCTTAGAGT ATCAGATGAGGCCCTTAGGCCCATCAGTGATGGGCCTGGCAGCAGGGGTATCAGGGACCTCTCAGATTTC CCACCGGGGAATTACCTCAGCTTTCCCCACCACTGCAAGCAGCGAGCCAGACTGGAGGGACTTCCGTCCT CTGGAGAAGCGTTATGAAGGAACCTGCTCCAAGAAAGATCAAAGTACCTGCCTGATGCAGCTCTTCCAGA GTGATGCCATGTTTGAGCCAGACATGCAAGAAGCAAATTTTGGAGGATCTCCCAGGAGGGCCTACCCTAC TTATTCACCCCCTGAAGATCCAGAGGAAGAGGAGGTTGAGAAGGAAGGGAATGCCACTGTGAGTTTCTCA CAGGCCCTGGTAGAGTTCACCAGCAATGGGAACCTCTTTTCCAGCATGTCCTGCAGCTCTGACTCTGACT CATCTTTCACTCAAAACCTCCCTGAGCTGCCTCCCATGGTGACCTTTGACATCGCTGATGTGGAACGGGA TGGGGAAGGCAAGTGTGAAGAGAATCCTGAGTTCCACAATGATGAAGATCTTGCAGCCTCCTTGGAAGCC TTTGAGCTGGGCTACTATCACAAACATGCCTTCAACAACTACCATAGTCGATTCTACCAAGGCCTGCCCT GGGGTGTGAGCAGCCTCCCTCGATACTTGGGACTGCCTGGCCTGCACCCTCGACCTCCACCTGCTGCTAT GGCCCTCAACAGGAGAAGCCGCTCTCTCGACACTGCAGAGACCCTGGAGATGGAGCTCTCCAATTCCCAC TTGGTCCAGGGCTACCTCGAGTCTGATGAGCTGCAGGCCCAGCAGGAAGATTCAGATGAGGAGGACGAAG AGGAGGAAGAAGGAGAATGGAGCCGAGACAGTCCCCTTTCCCTCTATACTGAACCCCCAGGGGCCTATGA TTGGCCTGCTTGGGCTCCCTGTCCTCTTCCAGTGGGGCCAGGTCCTGCCTGGATAAGCCCCAACCAGTTG GACAGGCCTTCCAGCCAGTCTCCATATAGGCAGGCAACCTGTTGCATACCTCCTATGACCATGTCAATAT CACTATCAGTGCCAGAGTCAAGGGCACCTGGGGAATCTGGGCCTCAACTAGCTCGTCCCTCACACCTACA CCTGCCCATGGGCCCTTGCTATAACCTCCAGCCACAGGCCTCCCAGAGCATGAGGGCCAGGCCTCGAGAT GTGCTGCTGCCTGTTGATGAGCCCAGTTGCTCTTCCAGTTCTGGAGGCTTCAGCCCCAGCCCTCTACCAC AGGCCAAGCCTGTGGGCATCACCCATGGCATTCCTCAGCTGCCCAGGGTCCGGCCTGAGCACCCCCAGCC TCAGCCCACTCACTATGGGCCTTCCAGCCTTGACCTGTCAAAGGAGAGGGCTGAGCAAGGTGCCTCTCTT GCCACCAGCTACTCCTCCACTGCCATGAATGGAAACCTAGCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC214530 representing NM_152424 Red=Cloning site Green=Tags(s) METQKDEAAQAKGAAASGSTREQTAEKGAKNKAAEATEGPTSEPSSSGPGRLKKTAMKLFGGKKGICTLP SFFGGGRSKGSGKGSSKKGLSKSKTHDGLSEAAHGPEDVVSEGTGFSLPLPELPCQFPSSQSAHGALETG SRCKTSVAGATEKAVAEKFPSMPKPKKGLKGFFSSIRRHRKSKVTGAEQSEPGAKGPERVRARPHEHVSS APQVPCFEETFQAPRKENANPQDAPGPKVSPTPEPSPPATEKMACKDPEKPMEACASAHVQPKPAPEASS LEEPHSPETGEKVVAGEVNPPNGPVGDPLSLLFGDVTSLKSFDSLTGCGDIIAEQDMDSMTDSMASGGQR ANRDGTKRSSCLVTYQGGGEEMALPDDDDEEEEEEEEVELEEEEEEVKEEEEDDDLEYLWETAQMYPRPN MNLGYHPTTSPGHHGYMLLDPVRSYPGLAPGELLTPQSDQQESAPNSDEGYYDSTTPGFEDDSGEALGLV RRDCLPRDSYSGDALYEFYEPDDSLENSPPGDDCLYDLHGRSSEMFDPFLNFEPFLSSRPPGAMETEEER LVTIQKQLLYWELRREQLEAQEARAREAHAREAHAREAYTREAYGREAYAREAHTWEAHGREARTREAQA REVRCRETQVRETQARQEKPVLEYQMRPLGPSVMGLAAGVSGTSQISHRGITSAFPTTASSEPDWRDFRP LEKRYEGTCSKKDQSTCLMQLFQSDAMFEPDMQEANFGGSPRRAYPTYSPPEDPEEEEVEKEGNATVSFS QALVEFTSNGNLFSSMSCSSDSDSSFTQNLPELPPMVTFDIADVERDGEGKCEENPEFHNDEDLAASLEA FELGYYHKHAFNNYHSRFYQGLPWGVSSLPRYLGLPGLHPRPPPAAMALNRRSRSLDTAETLEMELSNSH LVQGYLESDELQAQQEDSDEEDEEEEEGEWSRDSPLSLYTEPPGAYDWPAWAPCPLPVGPGPAWISPNQL DRPSSQSPYRQATCCIPPMTMSISLSVPESRAPGESGPQLARPSHLHLPMGPCYNLQPQASQSMRARPRD VLLPVDEPSCSSSSGGFSPSPLPQAKPVGITHGIPQLPRVRPEHPQPQPTHYGPSSLDLSKERAEQGASL ATSYSSTAMNGNLAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_152424
ORF Size	3405 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_152424.4
RefSeq Size	8443 bp
RefSeq ORF	3408 bp
Locus ID	139285
UniProt ID	Q5JTC6
MW	123.8 kDa
Gene Summary	The protein encoded by this gene upregulates trancriptional activation by the Wilms tumor protein and interacts with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2. Defects in this gene are a cause of osteopathia striata with cranial sclerosis (OSCS). [provided by RefSeq, May 2010]

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