PMPCB (NM_004279) Human Tagged ORF Clone
CAT#: RC215039
PMPCB (Myc-DDK-tagged)-Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_004279" in other vectors (6)
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CNY 3,656.00
CNY 4,280.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Beta-MPP; MAS1; MPP11; MPPB; MPPP52; P-52 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215039 representing NM_004279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCGGCGGCTCGAGTGGTGTTGTCATCCGCGGCGCGGCGGCGGCTCTGGGGTTTCAGCGAGA GTCTTCTAATCCGAGGCGCTGCGGGACGGTCATTATATTTTGGAGAGAACAGATTAAGAAGTACACAGGC TGCTACCCAAGTTGTTCTGAATGTTCCTGAAACAAGAGTAACATGTTTAGAAAGTGGACTCAGAGTAGCT TCGGAAGACTCTGGGCTCTCAACATGCACAGTTGGACTCTGGATTGATGCTGGAAGTAGATACGAAAATG AGAAGAACAATGGAACAGCACACTTTCTGGAGCATATGGCTTTCAAGGGCACCAAGAAGAGATCCCAGTT AGATCTGGAACTTGAGATTGAAAATATGGGTGCTCATCTCAATGCCTATACCTCCAGAGAGCAGACTGTA TACTATGCCAAAGCATTCTCTAAAGACTTGCCAAGAGCTGTAGAAATTCTTGCTGATATAATACAAAACA GCACATTGGGAGAAGCAGAGATTGAACGTGAGCGTGGAGTAATCCTTAGAGAGATGCAGGAAGTTGAAAC CAATTTACAAGAAGTTGTTTTTGATTATCTTCATGCCACAGCTTATCAAAATACTGCACTTGGACGGACA ATTTTGGGACCAACTGAAAATATCAAATCTATAAGTCGTAAGGACTTAGTGGATTATATAACCACACATT ATAAGGGGCCAAGAATAGTGCTTGCTGCTGCTGGAGGTGTTTCCCATGATGAATTGCTTGACTTAGCAAA GTTTCATTTCGGTGACTCTTTATGCACACACAAAGGAGAAATACCAGCTCTGCCTCCCTGCAAATTCACA GGAAGTGAGATTCGTGTGAGGGATGACAAGATGCCTTTGGCGCACCTTGCAATAGCTGTTGAAGCTGTTG GTTGGGCACATCCAGATACAATCTGTCTCATGGTTGCAAACACGCTGATTGGCAACTGGGATCGCTCTTT TGGGGGAGGAATGAATTTATCTAGCAAGCTGGCCCAGCTCACTTGTCATGGCAATCTTTGCCATAGCTTT CAGTCTTTCAACACTTCCTACACAGATACAGGATTATGGGGACTGTATATGGTTTGTGAATCATCCACTG TTGCAGACATGCTACATGTTGTTCAAAAAGAATGGATGCGACTCTGTACAAGTGTCACAGAAAGTGAGGT TGCACGAGCCAGAAATCTTCTGAAAACAAACATGTTGTTGCAGCTTGATGGTTCAACTCCAATTTGTGAA GATATTGGTAGGCAAATGTTATGCTATAATAGAAGGATTCCCATCCCTGAGCTTGAAGCAAGAATTGATG CTGTGAATGCTGAGACAATTCGAGAAGTATGTACCAAATACATTTATAATAGGAGTCCAGCTATTGCTGC TGTTGGTCCCATTAAGCAACTACCAGATTTTAAACAGATACGCAGTAACATGTGTTGGCTTCGTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215039 representing NM_004279
Red=Cloning site Green=Tags(s) MAAAAARVVLSSAARRRLWGFSESLLIRGAAGRSLYFGENRLRSTQAATQVVLNVPETRVTCLESGLRVA SEDSGLSTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTV YYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRT ILGPTENIKSISRKDLVDYITTHYKGPRIVLAAAGGVSHDELLDLAKFHFGDSLCTHKGEIPALPPCKFT GSEIRVRDDKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHSF QSFNTSYTDTGLWGLYMVCESSTVADMLHVVQKEWMRLCTSVTESEVARARNLLKTNMLLQLDGSTPICE DIGRQMLCYNRRIPIPELEARIDAVNAETIREVCTKYIYNRSPAIAAVGPIKQLPDFKQIRSNMCWLRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004279 |
ORF Size | 1467 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004279.3 |
RefSeq Size | 1771 bp |
RefSeq ORF | 1470 bp |
Locus ID | 9512 |
UniProt ID | O75439 |
Domains | Peptidase_M16, Peptidase_M16_C |
Protein Families | Druggable Genome, Protease |
MW | 54.37 kDa |
Gene Summary | This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Genome-wide RNAi Screen identifies PMPCB as a therapeutic vulnerability in EpCAM+ hepatocellular carcinoma
,Takai, A;Dang, H;Oishi, N;Khatib, S;Martin, SP;Dominguez, DA;Luo, J;Bagni, R;Wu, X;Powell, K;Ye, QH;Jia, H;Qin, LX;Chen, J;Mitchell, GA;Luo, X;Thorgeirsson, SS;Wang, XW;,
Cancer Res.
,PubMed ID 30862714
[PMPCB]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215039L1 | Lenti ORF clone of Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC215039L2 | Lenti ORF clone of Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,180.00 |
|
RC215039L3 | Lenti ORF clone of Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,180.00 |
|
RC215039L4 | Lenti ORF clone of Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,180.00 |
|
RG215039 | PMPCB (tGFP-tagged) - Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
SC127012 | PMPCB (untagged)-Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |