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UBN1 (NM_001079514) Human Tagged ORF Clone
CAT＃: RC215072
UBN1 (Myc-DDK-tagged)-Human ubinuclein 1 (UBN1), transcript variant 2
ORF Plasmid: tGFP
"NM_001079514" in other vectors (4)
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CNY 7,896.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | VT; VT4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215072 representing NM_001079514
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAGCCCCACAGGGTCCAGTTCACCTCTCTCCCAGGTTCCCTGAATCCTGCGTTTTTGAAGAAGT CCCGGAAGGAGGAGGCTGGGGCAGGAGAACAGCATCAGGACTGTGAGCCGGCTGCAGCAGCTGTTCGGAT TACACTCACCCTCTTTGAACCAGATCACAAACGCTGCCCAGAGTTCTTCTACCCAGAGCTGGTGAAGAAT ATCCGAGGGAAGGTAAAAGGCCTTCAGCCTGGAGATAAGAAGAAAGATCTGTCAGATCCTTTCAATGACG AAGAAAAGGAAAGGCATAAAGTAGAGGCCCTTGCCCGAAAATTTGAAGAAAAATACGGTGGAAAGAAACG TAGAAAAGACCGAATACAGGACTTGATCGATATGGGGTATGGTTATGATGAATCCGACTCCTTCATCGAT AACTCTGAGGCGTATGATGAGCTTGTTCCTGCTTCTTTGACTACGAAGTATGGAGGATTTTACATTAACT CGGGAACCCTGCAGTTTAGACAAGCATCAGAGTCTGAAGATGACTTCATTAAAGAAAAGAAGAAAAAATC TCCAAAGAAGCGGAAGTTGAAGGAAGGTGGTGAGAAGATAAAGAAGAAGAAAAAAGATGACACTTATGAC AAGGAGAAGAAATCGAAAAAGTCCAAGTTTTCCAAAGCCGGCTTCACAGCCCTCAATGCCAGTAAGGAGA AGAAGAAGAAGAAATATTCTGGGGCTTTAAGCGTTAAAGAGATGCTAAAGAAATTTCAGAAAGAGAAAGA GGCTCAGAAAAAAAGGGAGGAGGAGCATAAGCCTGTTGCGGTCCCATCAGCGGAAGCTCAGGGCCTGCGG GAACTGGAGGGTGCCTCTGACCCCTTGCTCTCACTCTTTGGCTCTACTTCTGACAACGACTTGCTCCAGG CGGCCACTGCCATGGACTCGCTGACGGATTTGGACTTGGAGCATCTGCTCAGTGAGTCTCCAGAAGGAAG TCCCTTCCGAGATATGGATGATGGAAGTGATTCCCTTGGGGTGGGATTGGACCAGGAATTCAGGCAGCCC TCTTCTCTCCCCGAAGGCCTGCCAGCACCCCTGGAGAAGCGCGTTAAGGAGCTGGCTCAGGCTGCCAGAG CTGCTGAGGGGGAGAGCAGACAGAAGTTCTTCACCCAGGATATTAATGGAATCCTATTAGACATAGAGGC GCAGACTCGGGAGCTGAGCAGTCAGGTCCGCTCTGGGGTGTATGCCTATCTTGCGTCATTCCTGCCCTGC AGCAAGGATGCCCTGCTCAAGCGTGCTCGGAAACTTCACCTCTATGAACAGGGGGGCCGTCTGAAGGAGC CTCTCCAGAAGCTCAAGGAAGCCATTGGCAGGGCGATGCCAGAGCAGATGGCCAAGTACCAGGACGAATG CCAGGCACACACGCAGGCAAAGGTTGCCAAGATGCTGGAAGAGGAGAAAGACAAGGAGCAGAGGGACCGG ATTTGTTCGGATGAGGAAGAAGATGAAGAAAAAGGGGGCAGGAGGATAATGGGACCTCGGAAGAAGTTCC AATGGAATGATGAGATCAGGGAGCTACTGTGCCAGGTGGTGAAGATCAAACTGGAGAGCCAGGACCTGGA GAGGAACAACAAAGCCCAGGCTTGGGAGGACTGTGTGAAGGGCTTTCTGGATGCGGAAGTCAAGCCCCTC TGGCCCAAAGGCTGGATGCAGGCCAGAACTCTGTTTAAGGAGAGCAGACGAGGCCATGGGCACCTGACTT CAATCCTGGCCAAGAAGAAAGTTATGGCCCCTTCTAAAATCAAGGTGAAGGAATCGTCTACGAAGCCTGA TAAAAAGGTTTCTGTCCCATCAGGACAGATTGGTGGCCCCATTGCTTTGCCCTCAGATCACCAAACAGGA GGCCTGAGTATTGGGGCCTCGAGCAGGGAGCTCCCATCCCAGGCATCGGGCGGCCTTGCTAACCCTCCTC CTGTCAACCTGGAGGACTCATTGGATGAAGACTTGATCCGCAATCCAGCCTCCTCGGTGGAAGCCGTGTC CAAGGAATTGGCTGCATTGAATAGCAGAGCAGCTGGGAACTCTGAATTCACACTGCCTGCACCCTCAAAA GCACCTGCAGAAAAAGTTGGAGGCGTTTTATGTACAGAAGAAAAAAGGAACTTTGCGAAGCCTAGTCCTT CTGCTCCACCACCAGCTAGCTCTCTGCAGTCACCCCTCAATTTTCTGGCAGAACAGGCTCTGGCACTGGG GCAGTCCTCTCAGGAGAAAAAACCAGAGAGTTCTGGCTACAAAGAGCTGTCCTGCCAGGCTCCCCTCAAT AAGGGCCTGCCAGAAGTACATCAGTCCAAAGCTAAGCACCACAGCTTGCCACGGACGTCTCACGGGCCCC AAGTGGCAGTTCCTGTGCCTGGCCCCCAGGTCAAAGTCTTTCATGCCGGCACTCAGCAGCAGAAAAACTT CACGCCCCCATCTCCATTTGCCAATAAGCTCCAAGGCCCAAAGGCTTCTCCCACACAGTGTCATCGTTCC CTCCTGCAGTTAGTGAAGACAGCGGCCAAAGGCCAGGGCTTCCATCCCTCTGCACCAGCCACCTCAGGAG GCCTGTCAGCCTCCAGCAGCAGCTCTCACAAGACCCCAGCCTCGTCCTCTTCTGCCCTGAGCCATCCAGC AAAGCCACATTCAGTCAGCTCTGCAGGCTCATCTTACAAGAATAATCCCTTTGCCAGCTCAATCTCCAAA CATGGGGTTTCTTCTGGCAGCTCTTCCTCGGGAGGAACACCAGTCCAGAGTTCTGTTTCTGGGAGCCTGG TCCCTGGCATACAGCCTCCCTCCGTGGGACAGGCCACCAGCCGACCCGTCCCAAGTTCAGCAGGGAAAAA AATGCCTGTTTCCCAGAAGTTGACTCTGGTAGCCCCTCCAGGCGGTCCAAACGGAGATTCCAGTGGTGGG ACCCAGGGAGTGGCAAAGTTGCTGACCTCGCCGTCCCTAAAGCCCTCTGCAGTTAGTAGTGTGACATCGT CTACCTCCTTGTCAAAAGGAGCGAGTGGGACTGTGCTGCTGGCCGGCTCCTCTTTGATGGCTTCACCCTA CAAATCCAGCAGCCCAAAGCTGTCTGGGGCCATGAGCTCGAACTCCTTGGGAATTATAACCCCTGTCCCT ATTCCTGTCCATGTGCTCTCCTTCAGCGCTGACTCCTCTGCCAAAGCAGGGGTCTCCAAGGATGCCATCG TCACAGGCCCTGCCCCCGGGTCCTTCCACCATGGCCTTGGCCACAGTCTTCTGGCTGGCTTGCACTCCAG CCCGCCCCATGCAGCGCCTCTCCCACACGCTGCGGTGCCCACCCATATCCCGCAGAGTCTGCCAGGTGCT TCTCAGCTTCACGGGAAAGGGCCTGCTGTACCACGGAAATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215072 representing NM_001079514
Red=Cloning site Green=Tags(s) MSEPHRVQFTSLPGSLNPAFLKKSRKEEAGAGEQHQDCEPAAAAVRITLTLFEPDHKRCPEFFYPELVKN IRGKVKGLQPGDKKKDLSDPFNDEEKERHKVEALARKFEEKYGGKKRRKDRIQDLIDMGYGYDESDSFID NSEAYDELVPASLTTKYGGFYINSGTLQFRQASESEDDFIKEKKKKSPKKRKLKEGGEKIKKKKKDDTYD KEKKSKKSKFSKAGFTALNASKEKKKKKYSGALSVKEMLKKFQKEKEAQKKREEEHKPVAVPSAEAQGLR ELEGASDPLLSLFGSTSDNDLLQAATAMDSLTDLDLEHLLSESPEGSPFRDMDDGSDSLGVGLDQEFRQP SSLPEGLPAPLEKRVKELAQAARAAEGESRQKFFTQDINGILLDIEAQTRELSSQVRSGVYAYLASFLPC SKDALLKRARKLHLYEQGGRLKEPLQKLKEAIGRAMPEQMAKYQDECQAHTQAKVAKMLEEEKDKEQRDR ICSDEEEDEEKGGRRIMGPRKKFQWNDEIRELLCQVVKIKLESQDLERNNKAQAWEDCVKGFLDAEVKPL WPKGWMQARTLFKESRRGHGHLTSILAKKKVMAPSKIKVKESSTKPDKKVSVPSGQIGGPIALPSDHQTG GLSIGASSRELPSQASGGLANPPPVNLEDSLDEDLIRNPASSVEAVSKELAALNSRAAGNSEFTLPAPSK APAEKVGGVLCTEEKRNFAKPSPSAPPPASSLQSPLNFLAEQALALGQSSQEKKPESSGYKELSCQAPLN KGLPEVHQSKAKHHSLPRTSHGPQVAVPVPGPQVKVFHAGTQQQKNFTPPSPFANKLQGPKASPTQCHRS LLQLVKTAAKGQGFHPSAPATSGGLSASSSSSHKTPASSSSALSHPAKPHSVSSAGSSYKNNPFASSISK HGVSSGSSSSGGTPVQSSVSGSLVPGIQPPSVGQATSRPVPSSAGKKMPVSQKLTLVAPPGGPNGDSSGG TQGVAKLLTSPSLKPSAVSSVTSSTSLSKGASGTVLLAGSSLMASPYKSSSPKLSGAMSSNSLGIITPVP IPVHVLSFSADSSAKAGVSKDAIVTGPAPGSFHHGLGHSLLAGLHSSPPHAAPLPHAAVPTHIPQSLPGA SQLHGKGPAVPRKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001079514 |
| ORF Size | 3402 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001079514.3 |
| RefSeq Size | 5982 bp |
| RefSeq ORF | 3405 bp |
| Locus ID | 29855 |
| UniProt ID | Q9NPG3 |
| MW | 121.3 kDa |
| Gene Summary | Cellular senescence is a hallmark of tumor suppression and tissue aging. Senescent cells contain domains of heterochromatin, called senescence-associated heterochromatin foci (SAHF), that repress proliferation-promoting genes. The protein encoded by this gene binds to proliferation-promoting genes and is required for SAHF formation, enhancing methylation of histone H3. [provided by RefSeq, Oct 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215072L3 | Lenti ORF clone of Human ubinuclein 1 (UBN1), transcript variant 2, Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC215072L4 | Lenti ORF clone of Human ubinuclein 1 (UBN1), transcript variant 2, mGFP tagged |
CNY 13,210.00 |
|
| RG215072 | UBN1 (tGFP-tagged) - Human ubinuclein 1 (UBN1), transcript variant 2 |
CNY 12,450.00 |
|
| SC315646 | UBN1 (untagged)-Human ubinuclein 1 (UBN1), transcript variant 2 |
CNY 21,850.00 |
