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NLRP7 (NM_139176) Human Tagged ORF Clone
CAT＃: RC215117
NLRP7 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1
ORF Plasmid: tGFP
"NM_139176" in other vectors (6)
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CNY 7,024.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLR19.4; HYDM; NALP7; NOD12; PAN7; PYPAF3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215117 representing NM_139176
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCGCCCCAGCTAGAGTGGACTCTGCAGACCCTTCTGGAGCAGCTGAACGAGGATGAATTAAAGA GTTTCAAATCCCTTTTATGGGCTTTTCCCCTCGAAGACGTGCTACAGAAGACCCCATGGTCTGAGGTGGA AGAGGCTGATGGCAAGAAACTGGCAGAAATTCTGGTCAACACCTCCTCAGAAAATTGGATAAGGAATGCG ACTGTGAACATCTTGGAAGAGATGAATCTCACGGAATTGTGTAAGATGGCAAAGGCTGAGATGATGGAGG ACGGACAGGTGCAAGAAATAGATAATCCTGAGCTGGGAGATGCAGAAGAAGACTCGGAGTTAGCAAAGCC AGGTGAAAAGGAAGGATGGAGAAATTCAATGGAGAAACAGTCTTTGGTCTGGAAGAACACCTTTTGGCAA GGAGACATTGACAATTTCCATGACGACGTCACTCTGAGAAACCAACGGTTCATTCCATTCTTGAATCCCA GAACACCCAGGAAGCTAACACCTTACACGGTGGTGCTGCACGGCCCCGCAGGCGTGGGGAAAACCACGCT GGCCAAAAAGTGTATGCTGGACTGGACAGACTGCAACCTCAGCCCGACGCTCAGATACGCGTTCTACCTC AGCTGCAAGGAGCTCAGCCGCATGGGCCCCTGCAGTTTTGCAGAGCTGATCTCCAAAGACTGGCCTGAAT TGCAGGATGACATTCCAAGCATCCTAGCCCAAGCACAGAGAATCCTGTTCGTGGTCGATGGCCTTGATGA GCTGAAAGTCCCACCTGGGGCGCTGATCCAGGACATCTGCGGGGACTGGGAGAAGAAGAAGCCGGTGCCC GTCCTCCTGGGGAGTTTGCTGAAGAGGAAGATGTTACCCAGGGCAGCCTTGCTGGTCACCACGCGGCCCA GGGCACTGAGGGACCTCCAGCTCCTGGCGCAGCAGCCGATCTACGTAAGGGTGGAGGGCTTCCTGGAGGA GGACAGGAGGGCCTATTTCCTGAGACACTTTGGAGACGAGGACCAAGCCATGCGTGCCTTTGAGCTAATG AGGAGCAACGCGGCCCTGTTCCAGCTGGGCTCGGCCCCCGCGGTGTGCTGGATTGTGTGCACGACTCTGA AGCTGCAGATGGAGAAGGGGGAGGACCCGGTCCCCACCTGCCTCACCCGCACGGGGCTGTTCCTGCGTTT CCTCTGCAGCCGGTTCCCGCAGGGCGCACAGCTGCGGGGCGCGCTGCGGACGCTGAGCCTCCTGGCCGCG CAGGGCCTGTGGGCGCAGATGTCCGTGTTCCACCGAGAGGACCTGGAAAGGCTCGGGGTGCAGGAGTCCG ACCTCCGTCTGTTCCTGGACGGAGACATCCTCCGCCAGGACAGAGTCTCCAAAGGCTGCTACTCCTTCAT CCACCTCAGCTTCCAGCAGTTTCTCACTGCCCTGTTCTACGCCCTGGAGAAGGAGGAGGGGGAGGACAGG GACGGCCACGCCTGGGACATCGGGGACGTACAGAAGCTGCTTTCCGGAGAAGAAAGACTCAAGAACCCCG ACCTGATTCAAGTAGGACACTTCTTATTCGGCCTCGCTAACGAGAAGAGAGCCAAGGAGTTGGAGGCCAC TTTTGGCTGCCGGATGTCACCGGACATCAAACAGGAATTGCTGCAATGCAAAGCACATCTTCATGCAAAT AAGCCCTTATCCGTGACCGACCTGAAGGAGGTCTTGGGCTGCCTGTATGAGTCTCAGGAGGAGGAGCTGG CGAAGGTGGTGGTGGCCCCGTTCAAGGAAATTTCTATTCACCTGACAAATACTTCTGAAGTGATGCATTG TTCCTTCAGCCTGAAGCATTGTCAAGACTTGCAGAAACTCTCACTGCAGGTAGCAAAGGGGGTGTTCCTG GAGAATTACATGGATTTTGAACTGGACATTGAATTTGAAAGCTCAAACAGCAACCTCAAGTTTCTGGAAG TGAAACAAAGCTTCCTGAGTGACTCTTCTGTGCGGATTCTTTGTGACCACGTAACCCGTAGCACCTGTCA TCTGCAGAAAGTGGAGATTAAAAACGTCACCCCTGACACCGCGTACCGGGACTTCTGTCTTGCTTTCATT GGGAAGAAGACCCTCACGCACCTGACCCTGGCAGGGCACATCGAGTGGGAACGCACGATGATGCTGATGC TGTGTGACCTGCTCAGAAATCATAAATGCAACCTGCAGTACCTGAGGTTGGGAGGTCACTGTGCCACCCC GGAGCAGTGGGCTGAATTCTTCTATGTCCTCAAAGCCAACCAGTCCCTGAAGCACCTGCGTCTCTCAGCC AATGTGCTCCTGGATGAGGGTGCCATGTTGCTGTACAAGACCATGACACGCCCAAAACACTTCCTGCAGA TGTTGTCGTTGGAAAACTGTCGTCTTACAGAAGCCAGTTGCAAGGACCTTGCTGCTGTCTTGGTTGTCAG CAAGAAGCTGACACACCTGTGCTTGGCCAAGAACCCCATTGGGGATACAGGGGTGAAGTTTCTGTGTGAG GGCTTGAGTTACCCTGATTGTAAACTGCAGACCTTGGTGTTACAGCAATGCAGCATAACCAAGCTTGGCT GTAGATATCTCTCAGAGGCGCTCCAAGAAGCCTGCAGCCTCACAAACCTGGACTTGAGTATCAACCAGAT AGCTCGTGGATTGTGGATTCTCTGTCAGGCATTAGAGAATCCAAACTGTAACCTAAAACACCTACGCCTC TGGAGCTGCTCCCTCATGCCTTTCTATTGTCAGCATCTTGGATCTGCTCTCCTCAGCAATCAGAAGCTTG AAACTCTGGACCTGGGCCAGAATCATTTGTGGAAGAGTGGCATAATTAAGCTCTTTGGGGTTCTAAGACA AAGAACTGGATCCTTGAAGATACTCAGGTTGAAGACCTATGAAACTAATTTGGAAATCAAGAAGCTGTTG GAGGAAGTGAAAGAAAAGAATCCCAAGCTGACTATTGATTGCAATGCTTCCGGGGCAACGGCACCTCCGT GCTGTGACTTTTTTTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC215117 representing NM_139176
Red=Cloning site Green=Tags(s) MTSPQLEWTLQTLLEQLNEDELKSFKSLLWAFPLEDVLQKTPWSEVEEADGKKLAEILVNTSSENWIRNA TVNILEEMNLTELCKMAKAEMMEDGQVQEIDNPELGDAEEDSELAKPGEKEGWRNSMEKQSLVWKNTFWQ GDIDNFHDDVTLRNQRFIPFLNPRTPRKLTPYTVVLHGPAGVGKTTLAKKCMLDWTDCNLSPTLRYAFYL SCKELSRMGPCSFAELISKDWPELQDDIPSILAQAQRILFVVDGLDELKVPPGALIQDICGDWEKKKPVP VLLGSLLKRKMLPRAALLVTTRPRALRDLQLLAQQPIYVRVEGFLEEDRRAYFLRHFGDEDQAMRAFELM RSNAALFQLGSAPAVCWIVCTTLKLQMEKGEDPVPTCLTRTGLFLRFLCSRFPQGAQLRGALRTLSLLAA QGLWAQMSVFHREDLERLGVQESDLRLFLDGDILRQDRVSKGCYSFIHLSFQQFLTALFYALEKEEGEDR DGHAWDIGDVQKLLSGEERLKNPDLIQVGHFLFGLANEKRAKELEATFGCRMSPDIKQELLQCKAHLHAN KPLSVTDLKEVLGCLYESQEEELAKVVVAPFKEISIHLTNTSEVMHCSFSLKHCQDLQKLSLQVAKGVFL ENYMDFELDIEFESSNSNLKFLEVKQSFLSDSSVRILCDHVTRSTCHLQKVEIKNVTPDTAYRDFCLAFI GKKTLTHLTLAGHIEWERTMMLMLCDLLRNHKCNLQYLRLGGHCATPEQWAEFFYVLKANQSLKHLRLSA NVLLDEGAMLLYKTMTRPKHFLQMLSLENCRLTEASCKDLAAVLVVSKKLTHLCLAKNPIGDTGVKFLCE GLSYPDCKLQTLVLQQCSITKLGCRYLSEALQEACSLTNLDLSINQIARGLWILCQALENPNCNLKHLRL WSCSLMPFYCQHLGSALLSNQKLETLDLGQNHLWKSGIIKLFGVLRQRTGSLKILRLKTYETNLEIKKLL EEVKEKNPKLTIDCNASGATAPPCCDFFC SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139176 |
| ORF Size | 3027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139176.3, NP_631915.2 |
| RefSeq Size | 3331 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 199713 |
| UniProt ID | Q8WX94 |
| MW | 114.7 kDa |
| Gene Summary | This gene encodes a member of the NACHT, leucine rich repeat, and PYD containing (NLRP) protein family. It has an N-terminal pyrin domain, followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. NLRP proteins are implicated in the activation of proinflammatory caspases through multiprotein complexes called inflammasomes. This gene may act as a feedback regulator of caspase-1-dependent interleukin 1-beta secretion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215117L1 | Lenti-ORF clone of NLRP7 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1 |
CNY 11,970.00 |
|
| RC215117L2 | Lenti-ORF clone of NLRP7 (mGFP-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1 |
CNY 11,970.00 |
|
| RC215117L3 | Lenti-ORF clone of NLRP7 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1 |
CNY 11,970.00 |
|
| RC215117L4 | Lenti-ORF clone of NLRP7 (mGFP-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1 |
CNY 11,970.00 |
|
| RG215117 | NLRP7 (tGFP-tagged) - Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1 |
CNY 11,120.00 |
|
| SC306130 | NLRP7 (untagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1 |
CNY 19,380.00 |
