TMEM16K (ANO10) (NM_018075) Human Tagged ORF Clone
CAT#: RC215283
ANO10 (Myc-DDK-tagged)-Human anoctamin 10 (ANO10), transcript variant 1
ORF Plasmid: tGFP
"NM_018075" in other vectors (7)
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CNY 4,912.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SCAR10; TMEM16K |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215283 representing NM_018075
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGACCTTATCAGCTTTGGATACTTCTGAGAGTTCTTTCACACCTTTGGTGGTCATAGAACTTG CTCAGGATGTCAAAGAAGAAACCAAAGAATGGCTGAAAAACAGAATTATAGCTAAAAAAAAAGATGGAGG TGCCCAGTTGTTGTTTAGACCATTGTTAAATAAATATGAACAAGAAACACTAGAAAATCAGAACTTATAT CTTGTTGGTGCCTCCAAGATTAGAATGTTACTAGGGGCAGAAGCAGTGGGATTGGTAAAAGAGTGCAATG ATAACACCATGAGAGCCTTCACATACAGAACCAGACAGAACTTCAAAGGTTTTGATGATAACAATGATGA TTTCCTGACAATGGCAGAATGTCAATTCATTATCAAACATGAACTTGAAAATCTTAGAGCTAAAGATGAA AAAATGATCCCTGGTTACCCTCAGGCAAAGTTGTATCCAGGAAAATCATTGTTGAGAAGATTGCTCACGT CTGGCATCGTGATTCAGGTGTTTCCACTGCATGACAGTGAAGCCCTGAAGAAGCTTGAGGACACCTGGTA CACTCGGTTTGCTTTGAAGTATCAGCCCATAGACAGTATTCGTGGCTACTTTGGGGAAACAATTGCTCTG TACTTTGGATTTTTGGAGTATTTCACTTTTGCATTAATCCCCATGGCTGTCATTGGGTTACCTTACTACT TGTTTGTGTGGGAAGACTATGACAAGTACGTGATCTTTGCCTCGTTCAACCTCATCTGGTCCACGGTGAT TCTGGAACTGTGGAAGCGTGGCTGTGCCAACATGACCTACAGGTGGGGGACACTGCTCATGAAGAGAAAG TTTGAGGAGCCCCGGCCAGGATTTCATGGTGTCTTGGGTATCAATTCCATCACTGGGAAGGAGGAGCCTC TGTACCCCAGCTACAAGAGACAGTTGCGCATTTACCTGGTCTCCCTGCCATTCGTGTGCCTCTGCCTCTA TTTCTCACTGTATGTCATGATGATTTACTTCGACATGGAGGTTTGGGCCTTGGGTCTACATGAGAACAGC GGGTCTGAGTGGACCAGTGTCCTGTTGTATGTGCCCAGCATCATCTATGCCATTGTGATTGAGATCATGA ATCGTCTCTATCGATATGCTGCCGAGTTTTTAACTTCATGGGAGAATCACAGATTGGAATCTGCCTATCA GAACCATCTAATTCTGAAAGTTTTAGTGTTCAACTTCCTCAATTGCTTTGCCTCACTCTTCTATATTGCC TTTGTCTTGAAAGATATGAAGCTTTTGCGCCAGAGCTTGGCCACTCTCCTAATTACCTCCCAGATCCTCA ACCAAATTATGGAATCTTTTCTTCCTTATTGGCTCCAAAGGAAGCATGGTGTGCGGGTGAAGAGGAAGGT GCAGGCTTTAAAGGCAGACATTGATGCTACATTATATGAACAAGTCATCCTGGAAAAAGAAATGGGAACT TATTTGGGCACCTTTGATGATTACTTGGAGTTATTCCTGCAGTTTGGTTATGTGAGCCTTTTCTCCTGTG TTTACCCATTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAAGTAAATTCAGATGCCTTAAAAAT GTGCAGGGTCTTCAAACGTCCATTCTCAGAACCTTCAGCCAATATTGGTGTGTGGCAGTTGGCTTTTGAA ACGATGAGTGTTATATCTGTGGTCACTAACTGTGCGCTGATTGGAATGTCACCACAAGTGAATGCAGTCT TTCCAGAATCAAAAGCAGACCTCATTTTGATTGTAGTAGCAGTGGAGCACGCACTCCTGGCTTTAAAGTT TATACTTGCATTTGCCATACCTGATAAGCCACGGCATATCCAGATGAAACTAGCCAGACTGGAATTTGAG TCTTTGGAGGCACTCAAGCAGCAGCAAATGAAGCTCGTGACCGAGAACCTGAAGGAGGAACCAATGGAAA GCGGGAAGGAGAAGGCAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215283 representing NM_018075
Red=Cloning site Green=Tags(s) MKVTLSALDTSESSFTPLVVIELAQDVKEETKEWLKNRIIAKKKDGGAQLLFRPLLNKYEQETLENQNLY LVGASKIRMLLGAEAVGLVKECNDNTMRAFTYRTRQNFKGFDDNNDDFLTMAECQFIIKHELENLRAKDE KMIPGYPQAKLYPGKSLLRRLLTSGIVIQVFPLHDSEALKKLEDTWYTRFALKYQPIDSIRGYFGETIAL YFGFLEYFTFALIPMAVIGLPYYLFVWEDYDKYVIFASFNLIWSTVILELWKRGCANMTYRWGTLLMKRK FEEPRPGFHGVLGINSITGKEEPLYPSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEVWALGLHENS GSEWTSVLLYVPSIIYAIVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLILKVLVFNFLNCFASLFYIA FVLKDMKLLRQSLATLLITSQILNQIMESFLPYWLQRKHGVRVKRKVQALKADIDATLYEQVILEKEMGT YLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFSEPSANIGVWQLAFE TMSVISVVTNCALIGMSPQVNAVFPESKADLILIVVAVEHALLALKFILAFAIPDKPRHIQMKLARLEFE SLEALKQQQMKLVTENLKEEPMESGKEKAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018075 |
ORF Size | 1980 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018075.5 |
RefSeq Size | 2743 bp |
RefSeq ORF | 1983 bp |
Locus ID | 55129 |
UniProt ID | Q9NW15 |
Domains | DUF590 |
Protein Families | Transmembrane |
MW | 76.1 kDa |
Gene Summary | The transmembrane protein encoded by this gene belongs to the anoctamin family of calcium-activated chloride channels, also known as the transmembrane 16 family. The encoded protein contains eight transmembrane domains with cytosolic N- and C-termini. Defects in this gene may cause autosomal recessive spinocerebellar ataxia-10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215283L1 | Lenti ORF clone of Human anoctamin 10 (ANO10), transcript variant 1, Myc-DDK-tagged |
CNY 7,312.00 |
|
RC215283L2 | Lenti ORF clone of Human anoctamin 10 (ANO10), transcript variant 1, mGFP tagged |
CNY 8,460.00 |
|
RC215283L3 | Lenti ORF clone of Human anoctamin 10 (ANO10), transcript variant 1, Myc-DDK-tagged |
CNY 8,460.00 |
|
RC215283L4 | Lenti ORF clone of Human anoctamin 10 (ANO10), transcript variant 1, mGFP tagged |
CNY 8,460.00 |
|
RG215283 | ANO10 (tGFP-tagged) - Human anoctamin 10 (ANO10), transcript variant 1 |
CNY 6,512.00 |
|
SC315603 | ANO10 (untagged)-Human anoctamin 10 (ANO10), transcript variant 1 |
CNY 10,550.00 |
|
SC318119 | ANO10 (untagged)-Human anoctamin 10 (ANO10), transcript variant 1 |
CNY 10,550.00 |
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