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FCHSD1 (NM_033449) Human Tagged ORF Clone
CAT＃: RC215379
FCHSD1 (Myc-DDK-tagged)-Human FCH and double SH3 domains 1 (FCHSD1)
ORF Plasmid: tGFP
"NM_033449" in other vectors (4)
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CNY 5,056.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NWK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215379 representing NM_033449
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGCCGCCCCGAAAAGTGAAGCCGGCCCAGGAGGTGAAGCTTCGCTTCCTGGAACAGCTGAGCA TCCTTCAGACCTGGCAGCAGAGGGAGGCGGATCTGCTGGAGGACATCAGATCCTACAGCAAGCAGAGGGC AGCCATTGAACGGGAGTATGGGCAGGCACTCCAGAAACTGGCTGGCCCATTCCTGAAGAGGGAAGGGCAC CGGAGCGGTGAGATGGACAGCAGGGGCAGGACAGTGTTCGGTGCCTGGCGCTGCCTGCTGGATGCCACCG TGGCTGGGGGCCAAACCCGACTCCAGGCGTCTGACCGATACCGTGACCTAGCAGGGGGTACAGGGCGGAG CGCCAAGGAGCAGGTGCTTAGGAAGGGAACAGAGAACCTCCAGAGGGCGCAGGCTGAGGTGCTGCAGTCT GTCCGGGAGCTGAGCCGAAGTCGGAAGCTGTATGGGCAGCGGGAACGTGTGTGGGCCTTGGCACAGGAGA AGGCGGCTGATGTCCAGGCCAGGCTAAACCGAAGTGACCATGGGATCTTCCACTCTCGGACCAGTCTCCA GAAACTGAGCACCAAGCTGTCCGCCCAGTCAGCCCAGTACTCCCAGCAGCTGCAAGCAGCCCGCAATGAG TACCTGCTTAACTTGGTGGCTACCAATGCCCACCTCGACCATTACTACCAGGAGGAACTGCCAGCTCTGC TCAAGGCCCTGGTCAGTGAGCTGTCAGAGCACTTGAGGGACCCCCTGACCTCCCTGAGCCACACTGAGCT GGAAGCCGCAGAGGTCATCCTGGAGCATGCCCACCGCGGGGAGCAGACAACCTCCCAGGTAAGCTGGGAG CAAGACCTGAAGCTGTTTCTTCAGGAGCCTGGTGTATTTTCCCCCACCCCACCTCAGCAGTTTCAGCCAG CAGGGACTGATCAGGTGTGTGTCCTGGAGTGGGGAGCAGAAGGCGTGGCTGGCAAGAGTGGCCTGGAGAA AGAGGTTCAGCGCTTGACCAGCCGAGCTGCCCGTGACTACAAGATCCAGAACCATGGGCATCGGGTACTG CAACGACTGGAGCAGAGGCGGCAGCAGGCTTCAGAGCGGGAGGCTCCAAGCATAGAACAGAGGTTACAGG AAGTGCGAGAGAGCATCCGCCGGGCACAGGTGAGCCAGGTGAAGGGGGCTGCCCGGCTGGCCCTGCTGCA GGGGGCTGGCTTAGATGTGGAGCGCTGGCTGAAGCCAGCCATGACCCAGGCCCAGGATGAGGTGGAGCAG GAGCGGCGGCTCAGTGAGGCTCGGCTGTCCCAGAGGGACCTCTCTCCAACCGCTGAGGATGCTGAGCTTT CTGACTTTGAGGAATGTGAGGAGACGGGAGAGCTCTTTGAGGAGCCTGCCCCCCAAGCCCTGGCCACGAG GGCCCTCCCCTGCCCTGCACACGTGGTATTTCGCTATCAGGCAGGGCGTGAGGATGAGCTGACAATCACG GAGGGTGAGTGGCTGGAGGTCATAGAGGAGGGAGATGCTGACGAATGGGTCAAGGCTCGGAACCAGCACG GCGAGGTAGGCTTTGTCCCTGAGCGATATCTCAACTTCCCGGACCTCTCCCTCCCAGAGAGCAGCCAAGA CAGTGACAATCCCTGCGGGGCAGAGCCCACAGCATTCCTGGCACAGGCCCTGTACAGCTACACCGGACAG AGTGCAGAGGAGCTGAGCTTCCCTGAGGGGGCACTCATCCGTCTGCTGCCCCGGGCCCAAGATGGAGTAG ATGACGGCTTCTGGAGGGGAGAATTTGGGGGCCGTGTTGGGGTCTTCCCCTCCCTGCTGGTGGAAGAGCT GCTTGGCCCCCCAGGGCCACCTGAACTCTCTGACCCTGAACAGATGCTGCCGTCCCCTTCTCCTCCCAGC TTCTCCCCACCTGCACCTACCTCTGTGTTGGATGGGCCCCCTGCACCTGTCCTGCCTGGGGACAAAGCCC TGGACTTCCCTGGGTTCCTGGACATGATGGCACCTCGACTCAGGCCGATGCGTCCACCACCTCCCCCGCC GGCTAAAGCCCCGGATCCTGGCCACCCAGATCCCCTCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215379 representing NM_033449
Red=Cloning site Green=Tags(s) MQPPPRKVKPAQEVKLRFLEQLSILQTWQQREADLLEDIRSYSKQRAAIEREYGQALQKLAGPFLKREGH RSGEMDSRGRTVFGAWRCLLDATVAGGQTRLQASDRYRDLAGGTGRSAKEQVLRKGTENLQRAQAEVLQS VRELSRSRKLYGQRERVWALAQEKAADVQARLNRSDHGIFHSRTSLQKLSTKLSAQSAQYSQQLQAARNE YLLNLVATNAHLDHYYQEELPALLKALVSELSEHLRDPLTSLSHTELEAAEVILEHAHRGEQTTSQVSWE QDLKLFLQEPGVFSPTPPQQFQPAGTDQVCVLEWGAEGVAGKSGLEKEVQRLTSRAARDYKIQNHGHRVL QRLEQRRQQASEREAPSIEQRLQEVRESIRRAQVSQVKGAARLALLQGAGLDVERWLKPAMTQAQDEVEQ ERRLSEARLSQRDLSPTAEDAELSDFEECEETGELFEEPAPQALATRALPCPAHVVFRYQAGREDELTIT EGEWLEVIEEGDADEWVKARNQHGEVGFVPERYLNFPDLSLPESSQDSDNPCGAEPTAFLAQALYSYTGQ SAEELSFPEGALIRLLPRAQDGVDDGFWRGEFGGRVGVFPSLLVEELLGPPGPPELSDPEQMLPSPSPPS FSPPAPTSVLDGPPAPVLPGDKALDFPGFLDMMAPRLRPMRPPPPPPAKAPDPGHPDPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033449 |
| ORF Size | 2070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033449.3 |
| RefSeq Size | 4333 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 89848 |
| UniProt ID | Q86WN1 |
| MW | 76.8 kDa |
| Gene Summary | Promotes actin polymerization mediated by SNX9 and WASL.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215379L3 | Lenti ORF clone of Human FCH and double SH3 domains 1 (FCHSD1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC215379L4 | Lenti ORF clone of Human FCH and double SH3 domains 1 (FCHSD1), mGFP tagged |
CNY 7,700.00 |
|
| RG215379 | FCHSD1 (tGFP-tagged) - Human FCH and double SH3 domains 1 (FCHSD1) |
CNY 6,370.00 |
|
| SC305637 | FCHSD1 (untagged)-Human FCH and double SH3 domains 1 (FCHSD1) |
CNY 13,300.00 |
