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TRIM71 (NM_001039111) Human Tagged ORF Clone
CAT＃: RC215451
TRIM71 (Myc-DDK-tagged)-Human tripartite motif containing 71 (TRIM71)
ORF Plasmid: tGFP
"NM_001039111" in other vectors (4)
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CNY 9,536.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HYDCC1; LIN-41; LIN41 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215451 representing NM_001039111
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGTTCCCCGAGACCGATTTCCAGATCTGCTTGCTGTGCAAGGAGATGTGCGGCTCGCCGGCGC CGCTCTCCTCCAACTCGTCCGCGTCGTCGTCCTCCTCGCAGACGTCCACGTCGTCGGGGGGCGGCGGCGG GGGCCCTGGGGCGGCGGCGCGCCGCCTACACGTCCTGCCCTGCCTGCACGCCTTCTGCCGCCCCTGCCTC GAGGCGCACCGGCTGCCGGCGGCGGGCGGCGGCGCGGCGGGAGAGCCGCTCAAGCTGCGCTGCCCCGTGT GCGACCAGAAAGTAGTGCTAGCCGAGGCGGCGGGTATGGACGCGCTGCCTTCGTCCGCCTTCCTGCTTAG CAACCTGCTCGACGCGGTGGTGGCCACTGCCGACGAGCCGCCGCCCAAGAACGGGCGCGCCGGCGCTCCG GCGGGAGCGGGCGGCCACAGCAACCACCGGCACCACGCTCACCACGCGCACCCGCGCGCGTCCGCCTCCG CGCCGCCACTCCCGCAGGCGCCGCAGCCGCCCGCGCCTTCCCGCTCGGCACCCGGCGGCCCTGCCGCTTC CCCGTCGGCGCTGCTGCTCCGCCGTCCTCACGGCTGCAGCTCGTGCGATGAGGGCAACGCAGCTTCTTCG CGCTGCCTCGACTGCCAGGAGCACCTGTGCGACAACTGCGTCCGAGCGCACCAGCGCGTGCGCCTCACCA AGGACCACTACATCGAGCGCGGCCCGCCGGGTCCCGGTGCCGCAGCAGCGGCGCAGCAGCTCGGGCTCGG GCCGCCCTTTCCCGGCCCGCCCTTCTCCATCCTCTCAGTGTTTCCCGAGCGCCTCGGCTTCTGCCAGCAC CACGACGACGAGGTGCTGCACCTGTACTGTGACACTTGCTCTGTACCCATCTGTCGTGAGTGCACAATGG GCCGGCATGGGGGCCACAGCTTCATCTACCTCCAGGAGGCACTGCAGGACTCACGGGCACTCACCATCCA GCTGCTGGCAGATGCCCAGCAGGGACGACAGGCAATCCAGCTGAGCATCGAGCAGGCCCAGACGGTGGCG GAACAGGTGGAGATGAAGGCGAAGGTTGTGCAGTCGGAGGTCAAAGCCGTGACGGCGAGGCATAAGAAAG CCCTGGAGGAACGCGAGTGTGAGCTGCTGTGGAAGGTAGAAAAGATCCGCCAGGTGAAAGCCAAGTCTCT GTACCTGCAGGTGGAGAAGCTGCGGCAAAACCTCAACAAGCTTGAGAGCACCATCAGTGCCGTGCAGCAG GTCCTGGAGGAGGGTAGAGCGCTAGACATCCTACTGGCCCGAGACCGGATGCTGGCCCAGGTGCAGGAGC TGAAGACCGTGCGGAGCCTCCTGCAGCCCCAGGAAGACGACCGAGTCATGTTCACACCCCCCGATCAGGC ACTGTACCTTGCCATCAAGTCTTTTGGCTTTGTTAGCAGCGGGGCCTTTGCCCCACTCACCAAGGCCACA GGCGATGGCCTCAAGCGTGCCCTCCAGGGTAAGGTGGCCTCCTTCACAGTCATTGGTTATGACCACGATG GTGAGCCCCGCCTCTCAGGAGGCGACCTGATGTCGGCTGTGGTCCTGGGCCCTGATGGCAACCTGTTTGG TGCAGAGGTGAGTGATCAGCAGAATGGGACATACGTGGTGAGTTACCGACCCCAGCTGGAGGGTGAGCAC CTGGTATCTGTGACACTGTGCAACCAGCACATTGAGAACAGCCCTTTCAAGGTGGTGGTCAAGTCAGGCC GCAGCTACGTGGGCATTGGGCTCCCGGGCCTGAGCTTCGGCAGTGAGGGTGACAGCGATGGCAAGCTCTG CCGCCCTTGGGGTGTGAGTGTAGACAAGGAGGGCTACATCATTGTCGCCGACCGCAGCAACAACCGCATC CAGGTGTTCAAGCCCTGCGGCGCCTTCCACCACAAATTCGGCACCCTGGGCTCCCGGCCTGGGCAGTTCG ACCGACCAGCCGGCGTGGCCTGTGACGCCTCACGCAGGATCGTGGTGGCTGACAAGGACAATCATCGCAT CCAGATCTTCACGTTCGAGGGCCAGTTCCTCCTCAAGTTTGGTGAGAAAGGAACCAAGAATGGGCAGTTC AACTACCCTTGGGATGTGGCGGTGAATTCTGAGGGCAAGATCCTGGTCTCAGACACGAGGAACCACCGGA TCCAGCTGTTTGGGCCTGATGGTGTCTTCCTAAACAAGTATGGCTTCGAGGGGGCTCTCTGGAAGCACTT TGACTCCCCACGGGGTGTGGCCTTCAACCATGAGGGCCACTTGGTGGTCACTGACTTCAACAACCACCGG CTCCTGGTTATTCACCCCGACTGCCAGTCGGCACGCTTTCTGGGCTCGGAGGGCACAGGCAATGGGCAGT TCCTGCGCCCACAAGGGGTAGCTGTGGACCAGGAAGGGCGCATCATTGTGGCGGATTCCAGGAACCATCG GGTACAGATGTTTGAATCCAACGGCAGCTTCCTGTGCAAGTTTGGTGCTCAAGGCAGCGGCTTTGGGCAG ATGGACCGCCCTTCCGGCATCGCCATCACCCCCGACGGAATGATCGTTGTGGTGGACTTTGGCAACAATC GAATCCTCGTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215451 representing NM_001039111
Red=Cloning site Green=Tags(s) MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSGGGGGGPGAAARRLHVLPCLHAFCRPCL EAHRLPAAGGGAAGEPLKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLLDAVVATADEPPPKNGRAGAP AGAGGHSNHRHHAHHAHPRASASAPPLPQAPQPPAPSRSAPGGPAASPSALLLRRPHGCSSCDEGNAASS RCLDCQEHLCDNCVRAHQRVRLTKDHYIERGPPGPGAAAAAQQLGLGPPFPGPPFSILSVFPERLGFCQH HDDEVLHLYCDTCSVPICRECTMGRHGGHSFIYLQEALQDSRALTIQLLADAQQGRQAIQLSIEQAQTVA EQVEMKAKVVQSEVKAVTARHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLESTISAVQQ VLEEGRALDILLARDRMLAQVQELKTVRSLLQPQEDDRVMFTPPDQALYLAIKSFGFVSSGAFAPLTKAT GDGLKRALQGKVASFTVIGYDHDGEPRLSGGDLMSAVVLGPDGNLFGAEVSDQQNGTYVVSYRPQLEGEH LVSVTLCNQHIENSPFKVVVKSGRSYVGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRI QVFKPCGAFHHKFGTLGSRPGQFDRPAGVACDASRRIVVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQF NYPWDVAVNSEGKILVSDTRNHRIQLFGPDGVFLNKYGFEGALWKHFDSPRGVAFNHEGHLVVTDFNNHR LLVIHPDCQSARFLGSEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFESNGSFLCKFGAQGSGFGQ MDRPSGIAITPDGMIVVVDFGNNRILVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039111 |
| ORF Size | 2604 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039111.3 |
| RefSeq Size | 3138 bp |
| RefSeq ORF | 2607 bp |
| Locus ID | 131405 |
| UniProt ID | Q2Q1W2 |
| MW | 93.2 kDa |
| Gene Summary | The protein encoded by this gene is an E3 ubiquitin-protein ligase that binds with miRNAs and maintains the growth and upkeep of embryonic stem cells. This gene also is involved in the G1-S phase transition of the cell cycle. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215451L3 | Lenti ORF clone of Human tripartite motif containing 71 (TRIM71), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC215451L4 | Lenti ORF clone of Human tripartite motif containing 71 (TRIM71), mGFP tagged |
CNY 9,030.00 |
|
| RG215451 | TRIM71 (tGFP-tagged) - Human tripartite motif containing 71 (TRIM71) |
CNY 7,890.00 |
|
| SC310844 | TRIM71 (untagged)-Human tripartite motif containing 71 (TRIM71) |
CNY 9,544.00 |
