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ETFB (NM_001014763) Human Tagged ORF Clone
CAT＃: RC215657
ETFB (Myc-DDK-tagged)-Human electron-transfer-flavoprotein, beta polypeptide (ETFB), transcript variant 2
ORF Plasmid: tGFP
"NM_001014763" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FP585; MADD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215657 representing NM_001014763
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCTCTCACTGTGGGTTACCATAAATACTGTAAATTTGAGAAACACTCTTTCAGGCCTGAGAGGAG CAGTGACAACAGTAGGGATGATCAAGTCAGATGTCCCTGGTACCCAGGAATGGCTGGATGAGAGAAGGAG ACAGGGTGACCTGCCCCTGCCTACAAACTCTAACCCAGTTCTGTCCCTGGAGCTGTGTGACCCCGGACAA GGCCCTGCCCCTTTCCAGGCTGTCGTTGTCCTCATCCAGCCTGGGAGAGGGTTGGCGCTGAGGCCCCCAC CTTCTTGCCTGTTTCCTCCTGACCCCACCCCTTCCCCTCCTGCTGGGCAGATCCGAGTGAAGCCTGACAG GACCGGTGTGGTCACGGATGGTGTGAAGCACTCCATGAACCCCTTCTGTGAGATCGCGGTGGAGGAGGCT GTGCGGCTCAAGGAGAAGAAGCTGGTGAAGGAGGTCATCGCCGTCAGCTGTGGGCCTGCACAGTGCCAGG AGACGATTCGTACCGCCCTGGCCATGGGTGCAGACCGAGGTATCCACGTGGAGGTGCCCCCAGCAGAAGC AGAACGCTTGGGTCCCCTGCAGGTGGCTCGGGTCCTGGCCAAGCTGGCAGAGAAGGAGAAGGTGGACCTG GTGCTGCTGGGCAAACAGGCCATCGATGATGACTGTAACCAGACAGGGCAGATGACAGCTGGATTTCTTG ACTGGCCACAGGGCACATTCGCCTCCCAGGTGACGCTGGAGGGGGACAAGTTGAAAGTGGAGCGGGAGAT CGATGGGGGCCTGGAGACCCTGCGCCTGAAGCTGCCAGCTGTGGTGACAGCTGACCTGAGGCTCAACGAG CCCCGCTACGCCACGCTGCCCAACATCATGAAAGCCAAGAAGAAGAAGATCGAGGTGATCAAGCCTGGGG ACCTGGGTGTGGACCTGACCTCCAAGCTCTCTGTGATCAGTGTGGAGGACCCGCCCCAGCGCACGGCCGG CGTCAAGGTGGAGACCACTGAGGACCTGGTGGCCAAGCTGAAGGAGATTGGGCGGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215657 representing NM_001014763
Red=Cloning site Green=Tags(s) MYLSLWVTINTVNLRNTLSGLRGAVTTVGMIKSDVPGTQEWLDERRRQGDLPLPTNSNPVLSLELCDPGQ GPAPFQAVVVLIQPGRGLALRPPPSCLFPPDPTPSPPAGQIRVKPDRTGVVTDGVKHSMNPFCEIAVEEA VRLKEKKLVKEVIAVSCGPAQCQETIRTALAMGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDL VLLGKQAIDDDCNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNE PRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014763 |
| ORF Size | 1038 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001014763.1, NP_001014763.1 |
| RefSeq Size | 1318 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 2109 |
| UniProt ID | P38117 |
| Protein Families | Druggable Genome |
| MW | 37.3 kDa |
| Gene Summary | This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215657L3 | Lenti ORF clone of Human electron-transfer-flavoprotein, beta polypeptide (ETFB), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215657L4 | Lenti ORF clone of Human electron-transfer-flavoprotein, beta polypeptide (ETFB), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG215657 | ETFB (tGFP-tagged) - Human electron-transfer-flavoprotein, beta polypeptide (ETFB), transcript variant 2 |
CNY 4,370.00 |
|
| SC301950 | ETFB (untagged)-Human electron-transfer-flavoprotein, beta polypeptide (ETFB), transcript variant 2 |
CNY 5,610.00 |
