p107 (RBL1) (NM_002895) Human Tagged Lenti ORF Clone
CAT#: RC215873L3
- LentiORF®
Lenti-ORF clone of RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1
"NM_002895" in other vectors (6)
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CNY 12,540.00
CNY 1,999.00
CNY 2,700.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CP107; p107; PRB1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC215873).
|
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002895 |
ORF Size | 3204 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002895.2 |
RefSeq Size | 4270 bp |
RefSeq ORF | 3207 bp |
Locus ID | 5933 |
UniProt ID | P28749 |
Domains | RB_B, RB_A |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Cell cycle, TGF-beta signaling pathway |
MW | 120.7 kDa |
Gene Summary | The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215873 | RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1 |
CNY 10,640.00 |
|
RC215873L1 | Lenti-ORF clone of RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1 |
CNY 12,540.00 |
|
RC215873L2 | Lenti-ORF clone of RBL1 (mGFP-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1 |
CNY 14,296.00 |
|
RC215873L4 | Lenti-ORF clone of RBL1 (mGFP-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1 |
CNY 12,540.00 |
|
RG215873 | RBL1 (tGFP-tagged) - Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1 |
CNY 13,496.00 |
|
SC109714 | RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1 |
CNY 11,168.00 |