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PREX2 (NM_025170) Human Tagged ORF Clone
CAT＃: RC215900
- TrueORF®
PREX2 (Myc-DDK-tagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 2
ORF Plasmid: tGFP
"NM_025170" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DEP.2; DEPDC2; P-REX2; PPP1R129 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215900 representing NM_025170
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGGACAGCCGCGGAGACAGCCGCGCCGAGAGCGCCAAGGACCTGGAGAAGCAGCTTCGCCTGC GCGTGTGCGTGCTCAGCGAGCTCCAGAAGACCGAGCGGGACTATGTGGGCACGCTGGAGTTCCTGGTGTC GGCATTCTTACACAGAATGAACCAGTGTGCAGCATCAAAAGTTGACAAAAATGTGACAGAAGAAACAGTG AAGATGTTGTTCTCAAACATTGAAGACATCCTTGCAGTACATAAAGAATTCTTAAAAGTCGTGGAAGAAT GCTTACACCCCGAACCTAATGCTCAACAAGAAGTGGGAACCTGCTTTCTTCACTTTAAAGACAAGTTTCG TATCTATGATGAATATTGTAGTAACCATGAGAAGGCACAAAAATTACTTCTTGAACTCAACAAAATAAGA ACAATCCGGACATTTCTTTTGAACTGCATGCTGCTTGGAGGACGGAAGAACACAGATGTTCCCTTGGAAG GATATTTAGTAACACCAATACAAAGAATATGCAAGTACCCTCTTATTTTGAAGGAGTTGCTGAAGCGGAC TCCACGGAAACACAGTGACTATGCAGCAGTGATGGAAGCCCTCCAAGCCATGAAAGCTGTCTGTTCCAAC ATAAACGAGGCCAAGAGACAGATGGAGAAGTTAGAAGTTTTAGAGGAATGGCAGTCTCACATTGAAGGCT GGGAGGGGTCCAACATCACTGACACCTGCACTGAAATGCTAATGTGTGGAGTCTTACTGAAAATTTCTTC TGGAAATATTCAAGAACGGGTGTTTTTTCTTTTCGATAATCTTTTGGTGTACTGCAAAAGAAAACACAGA CGGTTGAAGAACAGCAAGGCATCTACAGATGGACATCGGTACCTTTTTCGTGGCCGGATCAACACGGAGG TGATGGAAGTGGAGAATGTGGATGATGGCACCGCTGATTTCCATAGCAGTGGACACATTGTTGTTAATGG ATGGAAGATACATAACACAGCAAAAAATAAATGGTTTGTTTGTATGGCAAAAACACCTGAAGAGAAGCAT GAATGGTTTGAAGCTATTTTGAAAGAAAGAGAACGGCGGAAAGGTTTAAAATTAGGAATGGAGCAAGATA CCTGGGTCATGATCTCTGAACAGGGTGAGAAACTTTATAAAATGATGTGCAGACAAGGAAATCTGATCAA AGACCGAAAGAGAAAACTGACTACGTTCCCTAAATGCTTTCTTGGAAGCGAATTTGTGTCATGGCTGTTG GAAATTGGAGAGATTCACAGGCCTGAGGAAGGCGTGCACTTGGGACAAGCATTATTAGAAAATGGAATCA TTCACCATGTTACTGATAAACATCAATTCAAACCAGAACAGATGTTATATAGATTTCGCTATGATGATGG AACATTTTATCCAAGAAATGAGATGCAGGACGTGATTTCAAAGGGTGTAAGATTATATTGTCGTCTTCAT AGCCTTTTTACTCCAGTGATAAGAGATAAAGATTACCATTTAAGGACCTACAAATCTGTGGTCATGGCCA ACAAACTGATAGACTGGTTAATTGCACAGGGAGATTGCCGCACCAGAGAAGAGGCAATGATATTTGGCGT TGGACTCTGTGACAATGGATTTATGCACCATGTCCTTGAAAAAAGCGAATTCAAAGATGAACCCCTACTT TTCCGTTTTTTTTCGGATGAGGAAATGGAGGGATCAAATATGAAACATCGACTTATGAAACATGACTTAA AAGTTGTGGAAAATGTTATAGCTAAGTCATTATTGATTAAATCCAATGAAGGCAGCTATGGCTTTGGATT AGAAGACAAAAATAAAGTTCCAATAATAAAGTTGGTAGAAAAGGGATCTAATGCTGAGATGGCTGGCATG GAAGTCGGGAAAAAGATTTTTGCTATTAATGGTGACCTAGTTTTTATGAGACCTTTCAATGAAGTGGATT GCTTCCTGAAATCGTGTTTAAACAGCAGAAAACCTCTAAGAGTTCTTGTGAGCACAAAGCCAAGAGAGAC AGTGAAAATTCCAGATTCAGCTGATGGACTTGGCTTCCAGATCCGGGGATTTGGCCCTTCTGTTGTGCAT GCTGTAGGAAGAGGAACTGTGGCTGCAGCAGCTGGTCTTCACCCTGGACAGTGCATTATCAAGGTGAATG GAATCAATGTCAGCAAAGAGACACATGCCAGTGTCATTGCACACGTTACAGCCTGCAGGAAGTACAGGCG GCCAACGAAGCAAGATTCCATACAATGGGTTTATAATAGCATTGAGAGTGCTCAAGAAGACCTTCAAAAA TCTCACTCCAAGCCCCCTGGAGATGAAGCAGGGGATGCTTTTGACTGTAAAGTAGAAGAGGTTATTGACA AATTCAACACTATGGCCATCATTGATGGGAAGAAGGAGCATGTGAGTCTGACAGTGGACAATGTCCACCT GGAATATGGTGTCGTGTATGAGTACGACAGCACAGCTGGCATCAAGTGCAATGTGGTGGAAAAGATGATT GAGCCCAAAGGTTTCTTCAGCTTAACTGCCAAGATTCTTGAAGCCCTGGCTAAAAGTGATGAGCATTTTG TACAAAACTGTACCAGCCTAAATTCTCTAAATGAAGTGATTCCTACTGACCTTCAGAGTAAATTCAGTGC CCTTTGCAGTGAAAGAATTGAACACCTATGTCAGAGAATATCCAGTTATAAAAAGGTTCAAGCTTCAGAA AGGTTTTACAATTTCACAGCACGGCATGCTGTCTGGGAGCACAGTTTTGATCTTCACAGTGTAAGCAGCA CATTTCCAGTACCTGTGACTATGGAGTTCCTCCTGCTGCCTCCTCCTCTCTTAGGAATATCCCAGGATGG AAGACAGCACTGCATTCCTGAGGACCTTCCTTCTCAAGAAATGCTCTTAGCAGAGAGGGCCCCTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215900 representing NM_025170
Red=Cloning site Green=Tags(s) MSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASKVDKNVTEETV KMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIR TIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSN INEAKRQMEKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHR RLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKH EWFEAILKERERRKGLKLGMEQDTWVMISEQGEKLYKMMCRQGNLIKDRKRKLTTFPKCFLGSEFVSWLL EIGEIHRPEEGVHLGQALLENGIIHHVTDKHQFKPEQMLYRFRYDDGTFYPRNEMQDVISKGVRLYCRLH SLFTPVIRDKDYHLRTYKSVVMANKLIDWLIAQGDCRTREEAMIFGVGLCDNGFMHHVLEKSEFKDEPLL FRFFSDEEMEGSNMKHRLMKHDLKVVENVIAKSLLIKSNEGSYGFGLEDKNKVPIIKLVEKGSNAEMAGM EVGKKIFAINGDLVFMRPFNEVDCFLKSCLNSRKPLRVLVSTKPRETVKIPDSADGLGFQIRGFGPSVVH AVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVTACRKYRRPTKQDSIQWVYNSIESAQEDLQK SHSKPPGDEAGDAFDCKVEEVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNVVEKMI EPKGFFSLTAKILEALAKSDEHFVQNCTSLNSLNEVIPTDLQSKFSALCSERIEHLCQRISSYKKVQASE RFYNFTARHAVWEHSFDLHSVSSTFPVPVTMEFLLLPPPLLGISQDGRQHCIPEDLPSQEMLLAERAPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025170 |
| ORF Size | 2937 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025170.6 |
| RefSeq Size | 3024 bp |
| RefSeq ORF | 2940 bp |
| Locus ID | 80243 |
| UniProt ID | Q70Z35 |
| MW | 111.9 kDa |
| Gene Summary | The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215900L3 | Lenti-ORF clone of PREX2 (Myc-DDK-tagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 2 |
CNY 9,030.00 |
|
| RC215900L4 | Lenti-ORF clone of PREX2 (mGFP-tagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 2 |
CNY 9,030.00 |
|
| RG215900 | PREX2 (tGFP-tagged) - Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 2 |
CNY 7,890.00 |
|
| SC305242 | PREX2 (untagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 2 |
CNY 18,810.00 |
