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RP105 (CD180) (NM_005582) Human Tagged ORF Clone
CAT＃: RC215954
- TrueORF®
CD180 (Myc-DDK-tagged)-Human CD180 molecule (CD180)
ORF Plasmid: tGFP
"NM_005582" in other vectors (4)
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CNY 4,920.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LY64; Ly78; RP105 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215954 representing NM_005582
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTTGACGTCAGCTGCTTCTTTTGGGTGGTGCTGTTTTCTGCCGGCTGTAAAGTCATCACCTCCT GGGATCAGATGTGCATTGAGAAAGAAGCCAACAAAACATATAACTGTGAAAATTTAGGTCTCAGTGAAAT CCCTGACACTCTACCAAACACAACAGAATTTTTGGAATTCAGCTTTAATTTTTTGCCTACAATTCACAAT AGAACCTTCAGCAGACTCATGAATCTTACCTTTTTGGATTTAACTAGGTGCCAGATTAACTGGATACATG AAGACACTTTTCAAAGCCATCATCAATTAAGCACACTTGTGTTAACTGGAAATCCCCTGATATTCATGGC AGAAACATCGCTTAATGGGCCCAAGTCACTGAAGCATCTTTTCTTAATCCAAACGGGAATATCCAATCTC GAGTTTATTCCAGTGCACAATCTGGAAAACTTGGAAAGCTTGTATCTTGGAAGCAACCATATTTCCTCCA TTAAGTTCCCCAAAGACTTCCCAGCACGGAATCTGAAAGTACTGGATTTTCAGAATAATGCTATACACTA CATCTCTAGAGAAGACATGAGGTCTCTGGAGCAGGCCATCAACCTAAGCCTGAACTTCAATGGCAATAAT GTTAAAGGTATTGAGCTTGGGGCTTTTGATTCAACGATCTTCCAAAGTTTGAACTTTGGAGGAACTCCAA ATTTGTCTGTTATATTCAATGGTCTGCAGAACTCTACTACTCAGTCTCTCTGGCTGGGAACATTTGAGGA CATTGATGACGAAGATATTAGTTCAGCCATGCTCAAGGGACTCTGTGAAATGTCTGTTGAGAGCCTCAAC CTGCAGGAACACCGCTTCTCTGACATCTCATCCACCACATTTCAGTGCTTCACCCAACTCCAAGAATTGG ATCTGACAGCAACTCACTTGAAAGGGTTACCCTCTGGGATGAAGGGTCTGAACTTGCTCAAGAAATTAGT TCTCAGTGTAAATCATTTCGATCAATTGTGTCAAATCAGTGCTGCCAATTTCCCCTCCCTTACACACCTC TACATCAGAGGCAACGTGAAGAAACTTCACCTTGGTGTTGGCTGCTTGGAGAAACTAGGAAACCTTCAGA CACTTGATTTAAGCCATAATGACATAGAGGCTTCTGACTGCTGCAGTCTGCAACTCAAAAACCTGTCCCA CTTGCAAACCTTAAACCTGAGCCACAATGAGCCTCTTGGTCTCCAGAGTCAGGCATTCAAAGAATGTCCT CAGCTAGAACTCCTCGATTTGGCATTTACCCGCTTACACATTAATGCTCCACAAAGTCCCTTCCAAAACC TCCATTTCCTTCAGGTTCTGAATCTCACTTACTGCTTCCTTGATACCAGCAATCAGCATCTTCTAGCAGG CCTACCAGTTCTCCGGCATCTCAACTTAAAAGGGAATCACTTTCAAGATGGGACTATCACGAAGACCAAC CTACTTCAGACCGTGGGCAGCTTGGAGGTTCTGATTTTGTCCTCTTGTGGTCTCCTCTCTATAGACCAGC AAGCATTCCACAGCTTGGGAAAAATGAGCCATGTAGACTTAAGCCACAACAGCCTGACATGCGACAGCAT TGATTCTCTTAGCCATCTTAAGGGAATCTACCTCAATCTGGCTGCCAACAGCATTAACATCATCTCACCC CGTCTCCTCCCTATCTTGTCCCAGCAGAGCACCATTAATTTAAGTCATAACCCCCTGGACTGCACTTGCT CGAATATTCATTTCTTAACATGGTACAAAGAAAACCTGCACAAACTTGAAGGCTCGGAGGAGACCACGTG TGCAAACCCGCCATCTCTAAGGGGAGTTAAGCTATCTGATGTCAAGCTTTCCTGTGGGATTACAGCCATA GGCATTTTCTTTCTCATAGTATTTCTATTATTGTTGGCTATTCTGCTATTTTTTGCAGTTAAATACCTTC TCAGGTGGAAATACCAACACATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215954 representing NM_005582
Red=Cloning site Green=Tags(s) MAFDVSCFFWVVLFSAGCKVITSWDQMCIEKEANKTYNCENLGLSEIPDTLPNTTEFLEFSFNFLPTIHN RTFSRLMNLTFLDLTRCQINWIHEDTFQSHHQLSTLVLTGNPLIFMAETSLNGPKSLKHLFLIQTGISNL EFIPVHNLENLESLYLGSNHISSIKFPKDFPARNLKVLDFQNNAIHYISREDMRSLEQAINLSLNFNGNN VKGIELGAFDSTIFQSLNFGGTPNLSVIFNGLQNSTTQSLWLGTFEDIDDEDISSAMLKGLCEMSVESLN LQEHRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMKGLNLLKKLVLSVNHFDQLCQISAANFPSLTHL YIRGNVKKLHLGVGCLEKLGNLQTLDLSHNDIEASDCCSLQLKNLSHLQTLNLSHNEPLGLQSQAFKECP QLELLDLAFTRLHINAPQSPFQNLHFLQVLNLTYCFLDTSNQHLLAGLPVLRHLNLKGNHFQDGTITKTN LLQTVGSLEVLILSSCGLLSIDQQAFHSLGKMSHVDLSHNSLTCDSIDSLSHLKGIYLNLAANSINIISP RLLPILSQQSTINLSHNPLDCTCSNIHFLTWYKENLHKLEGSEETTCANPPSLRGVKLSDVKLSCGITAI GIFFLIVFLLLLAILLFFAVKYLLRWKYQHI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005582 |
| ORF Size | 1983 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005582.3 |
| RefSeq Size | 2725 bp |
| RefSeq ORF | 1986 bp |
| Locus ID | 4064 |
| UniProt ID | Q99467 |
| Domains | LRRCT, LRR, LRR_TYP |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 74.6 kDa |
| Gene Summary | CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215954L3 | Lenti-ORF clone of CD180 (Myc-DDK-tagged)-Human CD180 molecule (CD180) |
CNY 7,320.00 |
|
| RC215954L4 | Lenti-ORF clone of CD180 (mGFP-tagged)-Human CD180 molecule (CD180) |
CNY 5,890.00 |
|
| RG215954 | CD180 (tGFP-tagged) - Human CD180 molecule (CD180) |
CNY 6,520.00 |
|
| SC116668 | CD180 (untagged)-Human CD180 molecule (CD180) |
CNY 4,928.00 |
