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COL8A2 (NM_005202) Human Tagged ORF Clone
CAT＃: RC216003
COL8A2 (Myc-DDK-tagged)-Human collagen, type VIII, alpha 2 (COL8A2)
ORF Plasmid: tGFP
"NM_005202" in other vectors (4)
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CNY 5,152.00
CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FECD; FECD1; PPCD; PPCD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216003 representing NM_005202
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGACTCTGACACCCCTGTCTTCGCTGCTGCTGCTGCTACTGGTGCTGGTGCTGGGGTGTGGGC CGCGGGCGTCCTCTGGTGGCGGGGCCGGTGGGGCAGCGGGCTATGCCCCAGTGAAGTACATCCAGCCCAT GCAGAAAGGACCTGTGGGACCGCCCTTCCGTGAGGGCAAAGGCCAGTACCTGGAAATGCCTCTACCGCTG CTGCCGATGGACCTGAAGGGAGAGCCCGGCCCCCCTGGGAAGCCCGGGCCTCGGGGTCCCCCTGGCCCCC CTGGCTTCCCAGGAAAACCAGGCATGGGAAAGCCAGGACTCCATGGGCAGCCTGGCCCTGCTGGGCCCCC TGGCTTCTCCCGGATGGGCAAGGCTGGTCCCCCAGGGCTCCCTGGCAAGGTCGGGCCACCAGGGCAGCCG GGGCTTCGGGGGGAGCCAGGAATACGAGGGGACCAGGGCCTCCGGGGACCCCCAGGACCCCCTGGCCTCC CGGGCCCCTCAGGCATTACTATCCCTGGAAAACCAGGTGCCCAAGGGGTGCCAGGGCCCCCAGGATTCCA GGGGGAACCAGGGCCCCAGGGGGAGCCTGGGCCCCCAGGTGATCGAGGCCTCAAGGGGGATAATGGAGTG GGCCAGCCCGGGCTGCCTGGGGCCCCAGGGCAGGGGGGTGCCCCCGGCCCCCCCGGCCTCCCTGGTCCAG CTGGCTTAGGCAAACCTGGTTTGGATGGGCTTCCTGGGGCCCCAGGAGACAAGGGTGAGTCTGGGCCTCC TGGAGTTCCAGGCCCCAGGGGGGAGCCAGGAGCTGTGGGCCCAAAAGGACCTCCTGGAGTAGACGGTGTG GGAGTCCCAGGGGCAGCAGGGTTGCCAGGACCACAGGGCCCATCAGGGGCCAAAGGGGAGCCAGGGACCC GGGGCCCCCCTGGGCTGATAGGCCCCACTGGCTATGGGATGCCAGGACTGCCAGGCCCCAAGGGGGACAG GGGCCCAGCTGGGGTCCCAGGACTCTTGGGGGACAGGGGTGAGCCAGGGGAGGATGGGGAGCCAGGGGAG CAGGGCCCACAGGGTCTTGGGGGTCCCCCTGGACTTCCTGGGTCTGCAGGGCTTCCTGGCAGACGTGGGC CCCCTGGGCCTAAGGGTGAGGCAGGGCCTGGAGGACCCCCAGGAGTGCCTGGCATTCGAGGTGACCAGGG GCCTAGTGGCCTGGCTGGGAAACCAGGGGTCCCAGGTGAGAGGGGACTTCCTGGGGCCCATGGACCCCCT GGACCAACTGGGCCCAAGGGTGAGCCGGGTTTCACGGGTCGCCCTGGAGGACCAGGGGTGGCAGGAGCCC TGGGGCAGAAAGGTGACTTGGGGCTCCCTGGGCAGCCTGGCCTGAGGGGTCCCTCAGGAATCCCAGGACT CCAGGGTCCAGCTGGCCCTATTGGGCCCCAAGGCCTGCCGGGCCTGAAGGGGGAACCAGGCCTGCCAGGG CCCCCTGGAGAGGGAAGAGCAGGGGAACCTGGCACGGCTGGGCCCACGGGGCCCCCAGGGGTCCCTGGCT CCCCTGGAATCACGGGCCCTCCGGGGCCTCCCGGGCCCCCGGGACCCCCTGGTGCCCCTGGGGCCTTCGA TGAGACTGGCATCGCAGGCTTGCACCTGCCCAACGGCGGTGTGGAGGGTGCCGTGCTGGGCAAGGGGGGC AAGCCACAGTTTGGGCTGGGCGAGCTGTCTGCCCATGCCACACCGGCCTTCACTGCGGTGCTCACCTCGC CCTTCCCCGCCTCGGGCATGCCCGTGAAATTTGACCGGACTCTCTACAATGGCCACAGCGGCTACAACCC AGCCACTGGCATCTTCACCTGCCCTGTGGGCGGCGTCTACTACTTTGCTTACCATGTGCACGTCAAGGGC ACCAACGTGTGGGTGGCCCTGTACAAGAACAACGTGCCGGCCACCTATACCTACGATGAGTACAAGAAGG GCTACCTGGACCAGGCATCTGGTGGGGCCGTGCTCCAGCTGCGGCCCAACGACCAGGTCTGGGTGCAGAT GCCGTCGGACCAGGCCAACGGCCTCTACTCCACGGAGTACATCCACTCCTCCTTTTCAGGATTCTTGCTC TGCCCCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216003 representing NM_005202
Red=Cloning site Green=Tags(s) MLGTLTPLSSLLLLLLVLVLGCGPRASSGGGAGGAAGYAPVKYIQPMQKGPVGPPFREGKGQYLEMPLPL LPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQP GLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRGLKGDNGV GQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPKGPPGVDGV GVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGEPGEDGEPGE QGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGERGLPGAHGPP GPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPG PPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPNGGVEGAVLGKGG KPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKG TNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLL CPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005202 |
| ORF Size | 2109 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005202.4 |
| RefSeq Size | 4443 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 1296 |
| UniProt ID | P25067 |
| Protein Families | Druggable Genome |
| MW | 67.24 kDa |
| Gene Summary | This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216003L3 | Lenti ORF clone of Human collagen, type VIII, alpha 2 (COL8A2), Myc-DDK-tagged |
CNY 9,310.00 |
|
| RC216003L4 | Lenti ORF clone of Human collagen, type VIII, alpha 2 (COL8A2), mGFP tagged |
CNY 9,310.00 |
|
| RG216003 | COL8A2 (tGFP-tagged) - Human collagen, type VIII, alpha 2 (COL8A2) |
CNY 6,752.00 |
|
| SC127921 | COL8A2 (untagged)-Human collagen, type VIII, alpha 2 (COL8A2) |
CNY 13,590.00 |
