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PCGF1 (NM_032673) Human Tagged ORF Clone
CAT＃: RC216322
PCGF1 (Myc-DDK-tagged)-Human polycomb group ring finger 1 (PCGF1)
ORF Plasmid: tGFP
"NM_032673" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010002K04Rik; NSPC1; RNF3A-2; RNF68 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216322 representing NM_032673
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCGTCTCCTCAGGGGGGCCAGATTGCGATCGCGATGAGGCTTCGGAACCAGCTCCAGTCAGTGTACA AGATGGACCCGCTACGGAACGAGGAGGAGGTTCGAGTGAAGATCAAAGACTTGAATGAACACATTGTTTG CTGCCTATGCGCCGGCTACTTCGTGGATGCCACCACCATCACAGAGTGTCTTCATACTTTCTGCAAGAGT TGTATTGTGAAGTACCTCCAAACTAGCAAGTACTGCCCCATGTGCAACATTAAGATCCACGAGACACAGC CACTGCTCAACCTCAAACTGGACCGGGTCATGCAGGACATCGTGTATAAGCTGGTGCCTGGCTTGCAAGA CAGTGAAGAGAAACGGATTCGGGAATTCTACCAGTCCCGAGGTTTGGACCGGGTCACCCAGCCCACTGGG GAAGAGCCAGCACTGAGCAACCTCGGCCTCCCCTTCAGCAGCTTTGACCACTCTAAAGCCCACTACTATC GCTATGATGAGCAGTTGAACCTGTGCCTGGAGCGGCTGAGTTCTGGCAAAGACAAGAATAAAAGCGTCCT GCAGAACAAGTATGTCCGATGTTCTGTTAGAGCTGAGGTACGCCATCTCCGGAGGGTCCTGTGTCACCGC TTGATGCTAAACCCTCAGCATGTGCAGCTCCTTTTTGACAATGAAGTTCTCCCTGATCACATGACAATGA AGCAGATATGGCTCTCCCGCTGGTTCGGCAAGCCATCCCCTTTGCTTTTACAATACAGTGTGAAAGAGAA GAGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216322 representing NM_032673
Red=Cloning site Green=Tags(s) MASPQGGQIAIAMRLRNQLQSVYKMDPLRNEEEVRVKIKDLNEHIVCCLCAGYFVDATTITECLHTFCKS CIVKYLQTSKYCPMCNIKIHETQPLLNLKLDRVMQDIVYKLVPGLQDSEEKRIREFYQSRGLDRVTQPTG EEPALSNLGLPFSSFDHSKAHYYRYDEQLNLCLERLSSGKDKNKSVLQNKYVRCSVRAEVRHLRRVLCHR LMLNPQHVQLLFDNEVLPDHMTMKQIWLSRWFGKPSPLLLQYSVKEKRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032673 |
| ORF Size | 777 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032673.3 |
| RefSeq Size | 939 bp |
| RefSeq ORF | 780 bp |
| Locus ID | 84759 |
| UniProt ID | Q9BSM1 |
| Domains | RING |
| MW | 30.2 kDa |
| Gene Summary | PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216322L1 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216322L2 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), mGFP tagged |
CNY 5,890.00 |
|
| RC216322L3 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216322L4 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), mGFP tagged |
CNY 5,890.00 |
|
| RG216322 | PCGF1 (tGFP-tagged) - Human polycomb group ring finger 1 (PCGF1) |
CNY 4,370.00 |
|
| SC103969 | PCGF1 (untagged)-Human polycomb group ring finger 1 (PCGF1) |
CNY 2,400.00 |
|
| SC317369 | PCGF1 (untagged)-Human polycomb group ring finger 1 (PCGF1) |
CNY 3,990.00 |
