PIST (GOPC) (NM_020399) Human Tagged ORF Clone

CAT#: RC216529

GOPC (Myc-DDK-tagged)-Human golgi-associated PDZ and coiled-coil motif containing (GOPC), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_020399" in other vectors (6)

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CNY 3,656.00

CNY 4,090.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal PIST Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CAL; dJ94G16.2; FIG; GOPC1; PIST
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216529 representing NM_020399
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCGGGCGGTCCATGCCCAGCAGCAGCCGGAGGGGGCCCAGGGGGCGCCTCCTGCTCCGTGGGGG
CCCCTGGCGGGGTATCCATGTTCCGGTGGCTGGAGGTGCTGGAGAAGGAGTTCGACAAAGCTTTTGTGGA
TGTGGATCTGCTCCTGGGAGAGATCGATCCAGACCAAGCGGACATCACTTATGAGGGGCGACAGAAGATG
ACCAGCCTGAGCTCCTGCTTTGCACAGCTTTGCCACAAAGCCCAGTCTGTGTCTCAAATCAACCACAAGC
TGGAGGCACAGTTGGTGGATCTGAAATCTGAACTGACAGAAACCCAAGCAGAGAAAGTTGTTTTGGAGAA
AGAAGTACATGATCAGCTTTTACAGCTGCACTCTATTCAGCTGCAGCTTCATGCTAAAACTGGTCAAAGT
GCTGACTCTGGTACCATTAAGGCAAAATTGTCTGGCCCCTCTGTGGAGGAGCTGGAAAGAGAGCTTGAGG
CAAACAAAAAAGAAAAAATGAAAGAAGCACAACTTGAAGCTGAAGTGAAATTGTTGAGAAAAGAGAATGA
AGCCCTTCGTAGACATATAGCTGTTCTCCAGGCTGAAGTATATGGGGCGAGACTAGCTGCCAAGTACTTG
GATAAGGAACTGGCAGGAAGGGTCCAACAGATACAATTGCTAGGACGAGATATGAAGGGACCTGCTCATG
ATAAGCTTTGGAACCAATTAGAAGCTGAAATACATTTGCATCGTCACAAAACTGTGATCCGAGCCTGCAG
AGGACGTAATGACTTGAAACGACCAATGCAAGCACCACCAGGCCATGATCAAGATTCCCTAAAGAAAAGC
CAAGGTGTTGGTCCAATTAGAAAAGTTCTCCTCCTTAAGGAAGATCATGAAGGCCTTGGCATTTCAATTA
CAGGTGGGAAAGAACATGGTGTTCCAATCCTCATCTCTGAGATCCATCCGGGGCAACCTGCTGATAGATG
CGGAGGGCTGCACGTTGGGGATGCTATTTTGGCAGTCAACGGAGTTAACCTAAGGGACACAAAGCATAAA
GAAGCTGTAACTATTCTTTCTCAGCAGAGAGGAGAGATTGAATTTGAAGTAGTTTATGTGGCTCCTGAAG
TGGATTCTGATGATGAAAACGTAGAGTATGAAGATGAGAGTGGACATCGTTACCGTTTGTACCTTGATGA
GTTAGAAGGAGGTGGTAACCCTGGTGCTAGTTGCAAAGACACAAGTGGGGAAATCAAAGTATTACAAGGA
TTTAATAAGAAGGCAGTAACTGACACACATGAAAATGGAGACCTGGGCACTGCAAGTGAAACTCCGCTAG
ATGACGGTGCTTCAAAATTAGATGATCTGCACACTCTGTATCATAAAAAATCTTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216529 representing NM_020399
Red=Cloning site Green=Tags(s)

MSAGGPCPAAAGGGPGGASCSVGAPGGVSMFRWLEVLEKEFDKAFVDVDLLLGEIDPDQADITYEGRQKM
TSLSSCFAQLCHKAQSVSQINHKLEAQLVDLKSELTETQAEKVVLEKEVHDQLLQLHSIQLQLHAKTGQS
ADSGTIKAKLSGPSVEELERELEANKKEKMKEAQLEAEVKLLRKENEALRRHIAVLQAEVYGARLAAKYL
DKELAGRVQQIQLLGRDMKGPAHDKLWNQLEAEIHLHRHKTVIRACRGRNDLKRPMQAPPGHDQDSLKKS
QGVGPIRKVLLLKEDHEGLGISITGGKEHGVPILISEIHPGQPADRCGGLHVGDAILAVNGVNLRDTKHK
EAVTILSQQRGEIEFEVVYVAPEVDSDDENVEYEDESGHRYRLYLDELEGGGNPGASCKDTSGEIKVLQG
FNKKAVTDTHENGDLGTASETPLDDGASKLDDLHTLYHKKSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020399
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020399.4
RefSeq Size 4604 bp
RefSeq ORF 1389 bp
Locus ID 57120
UniProt ID Q9HD26
Domains PDZ
MW 50.3 kDa
Gene Summary This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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