United States
Germany
Japan
United Kingdom
China
HIF1 beta (ARNT) (NM_001668) Human Tagged ORF Clone
CAT＃: RC216724
ARNT (Myc-DDK-tagged)-Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1
ORF Plasmid: tGFP
"NM_001668" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,776.00
CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216724 representing NM_001668
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGACTACTGCCAACCCCGAAATGACATCAGATGTACCATCACTGGGTCCAGCCATTGCCTCTG GAAACTCTGGACCTGGAATTCAAGGTGGAGGAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCT GGATTTTGATGATGATGGAGAAGGGAACAGTAAATTTTTGAGGTGTGATGATGATCAGATGTCTAACGAT AAGGAGCGGTTTGCCAGGTCGGATGATGAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATC ACAGTGAAATTGAACGGCGGCGACGGAACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACC CACCTGTAGTGCCCTGGCTCGAAAACCAGACAAGCTAACCATCTTACGCATGGCAGTTTCTCACATGAAG TCCTTGCGGGGAACTGGCAACACATCCACTGATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAAC TGAAACATTTGATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTGTCTCATGTGAGACAGGCAGGGTGGT GTATGTGTCTGACTCCGTGACTCCTGTTTTGAACCAGCCACAGTCTGAATGGTTTGGCAGCACACTCTAT GATCAGGTGCACCCAGATGATGTGGATAAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAG GGCGTATCCTGGATCTAAAGACTGGAACAGTGAAAAAGGAAGGTCAGCAGTCTTCCATGAGAATGTGTAT GGGCTCAAGGAGATCGTTTATTTGCCGAATGAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAAT AGGCTGAGCTTTGTGAGGAACAGATGCAGGAATGGACTTGGCTCTGTAAAGGATGGGGAACCTCACTTCG TGGTGGTCCACTGCACAGGCTACATCAAGGCCTGGCCCCCAGCAGGTGTTTCCCTCCCAGATGATGACCC AGAGGCTGGCCAGGGAAGCAAGTTTTGCCTAGTGGCCATTGGCAGATTGCAGGTAACTAGTTCTCCCAAC TGTACAGACATGAGTAATGTTTGTCAACCAACAGAGTTCATCTCCCGACACAACATTGAGGGTATCTTCA CTTTTGTGGATCACCGCTGTGTGGCTACTGTTGGCTACCAGCCACAGGAACTCTTAGGAAAGAATATTGT AGAATTCTGTCATCCTGAAGACCAGCAGCTTCTAAGAGACAGCTTCCAACAGGTAGTGAAATTAAAAGGC CAAGTGCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACCAAGAATGGCTCTGGATGAGAACCAGCTCCT TTACTTTCCAGAACCCTTACTCAGATGAAATTGAGTACATCATCTGTACCAACACCAATGTGAAGAACTC TAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAGGCCACAACTAGGTCCCACAGCTAATTTA CCCCTGGAGATGGGCTCAGGACAGCTGGCACCCAGGCAGCAGCAACAGCAAACAGAATTGGACATGGTAC CAGGAAGAGATGGACTGGCCAGCTACAATCATTCCCAGGTGGTTCAGCCTGTGACAACCACAGGACCAGA ACACAGCAAGCCCCTTGAGAAGTCAGATGGTTTATTTGCCCAGGATAGAGATCCAAGATTTTCAGAAATC TATCACAACATCAATGCGGATCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTAT TCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGCCTAGCCCC TCCTGTAACCATTGTCCAGCCATCAGCTTCTGCAGGACAGATGTTGGCCCAGATTTCCCGCCACTCCAAC CCCACCCAAGGAGCAACCCCAACTTGGACCCCTACTACCCGCTCAGGCTTTTCTGCCCAGCAGGTGGCTA CCCAGGCTACTGCTAAGACTCGTACTTCCCAGTTTGGTGTGGGCAGCTTTCAGACTCCATCCTCCTTCAG CTCCATGTCCCTCCCTGGTGCCCCAACTGCATCGCCTGGTGCTGCTGCCTACCCTAGTCTCACCAATCGT GGATCTAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAATTCCAGACACGGACAGCAGAGGGTGTGG GTGTCTGGCCACAGTGGCAGGGCCAGCAGCCTCATCATCGTTCAAGTTCTAGTGAGCAACATGTTCAACA ACCGCCAGCACAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGATGCTGTCCATGCTGGGAGATCAGAGC AACAGCTACAACAATGAAGAATTCCCTGATCTAACTATGTTTCCCCCCTTTTCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216724 representing NM_001668
Red=Cloning site Green=Tags(s) MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSND KERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMK SLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLY DQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVN RLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPN CTDMSNVCQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKG QVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANL PLEMGSGQLAPRQQQQQTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEI YHNINADQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSN PTQGATPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNR GSNFAPETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQS NSYNNEEFPDLTMFPPFSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001668 |
| ORF Size | 2367 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001668.4 |
| RefSeq Size | 4846 bp |
| RefSeq ORF | 2370 bp |
| Locus ID | 405 |
| UniProt ID | P27540 |
| Domains | PAS, HLH, PAC |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Pathways in cancer, Renal cell carcinoma |
| MW | 86.5 kDa |
| Gene Summary | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MYC-associated protein X binding with the variant rs72780850 in RNA helicase DEAD box 1 for susceptibility to neuroblastoma.
,null,
Science China. Life sciences
,PubMed ID 32915406
[HIF1 beta]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216724L1 | Lenti ORF clone of Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1, Myc-DDK-tagged |
CNY 8,176.00 |
|
| RC216724L2 | Lenti ORF clone of Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC216724L3 | Lenti ORF clone of Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC216724L4 | Lenti ORF clone of Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG216724 | ARNT (tGFP-tagged) - Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1 |
CNY 7,376.00 |
|
| SC119100 | ARNT (untagged)-Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1 |
CNY 5,784.00 |
其它HIF1 beta产品
