MAP2 (NM_002374) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAP-2; MAP2A; MAP2B; MAP2C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216775 representing NM_002374. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGATGAACGGAAAGATGAAGCAAAGGCACCTCACTGGACCTCAGCACCGCTAACAGAGGCATCT GCACACTCACATCCACCTGAGATTAAGGATCAAGGCGGAGCAGGGGAAGGACTTGTCCGAAGCGCTAAT GGATTCCCATACAGGGAGGATGAAGAGGGTGCCTTTGGAGAGCATGGGTCACAGGGCACCTATTCAAAT ACCAAAGAGAATGGGATCAACGGAGAGCTGACCTCAGCTGACAGAGAAACAGCAGAGGAGGTGTCTGCA AGGATAGTTCAAGTAGTCACTGCTGAGGCTGTAGCAGTCCTGAAAGGTGAACAAGAGAAAGAAGCTCAA CATAAAGACCAGACTGCAGCTCTGCCTTTAGCAGCTGAAGAAACAGCTAATCTGCCTCCTTCTCCACCC CCATCACCTGCCTCAGAACAGACTGTCACAGTGGAGGAAGATTTACTTACAGCCTCGAAGATGGAGTTC CACGATCAACAGGAATTGACTCCCTCTACAGCTGAGCCTTCAGACCAGAAGGAAAAGGAGTCAGAGAAG CAAAGTAAGCCTGGTGAAGACCTTAAACATGCTGCCTTAGTTTCTCAGCCAGAGACAACTAAAACTTAC CCTGATAAAAAGGACATGCAAGGCACGGAAGAAGAAAAAGCACCCCTAGCTTTGTTTGGGCACACTCTT GTTGCCAGCCTGGAAGACATGAAACAGAAGACAGAACCAAGCCTTGTAGTACCTGGCATTGACCTCCCT AAAGAGCCTCCAACTCCAAAAGAACAAAAGGACTGGTTCATCGAAATGCCAACGGAAGCAAAAAAGGAT GAGTGGGGTTTAGTTGCCCCCATATCTCCTGGCCCTCTGACTCCCATGAGGGAAAAAGATGTATTTGAT GATATCCCAAAATGGGAAGGGAAACAGTTTGATTCTCCCATGCCAAGTCCCTTTCAAGGGGGAAGCTTC ACTCTTCCTTTAGATGTCATGAAGAATGAAATAGTTACAGAAACATCGCCCTTTGCCCCTGCCTTTTTA CAGCCAGATGACAAAAAATCTCTGCAACAAACCAGTGGCCCAGCTACTGCCAAAGATAGTTTTAAAATT GAAGAGCCCCATGAGGCTAAACCTGACAAAATGGCAGAAGCACCACCCTCAGAGGCAATGACCTTACCC AAAGATGCTCACATTCCAGTTGTAGAAGAACATGTTATGGGGAAAGTTTTAGAGGAAGAAAAGGAGGCC ATAAATCAAGAGACTGTGCAGCAAAGGGATACTTTCACCCCCAGTGGACAGGAACCTATACTTACTGAA AAGGAAACTGAGCTGAAGCTTGAAGAAAAAACCACCATTTCTGACAAAGAAGCTGTGCCAAAAGAGAGT AAACCCCCAAAACCTGCAGATGAAGAAATAGGCATAATTCAGACCTCCACAGAGCACACTTTCTCAGAA CAGAAAGACCAAGAGCCTACCACAGATATGTTGAAACAGGACTCGTTCCCTGTAAGTTTGGAGCAAGCA GTTACAGATTCAGCCATGACCTCTAAAACACTGGAGAAAGCCATGACCGAACCATCTGCATTAATTGAA AAGAGCTCAATTCAGGAACTTTTTGAAATGAGAGTTGATGACAAAGATAAGATTGAAGGAGTTGGAGCT GCAACATCAGCTGAGCTTGATATGCCATTTTATGAAGATAAATCAGGAATGTCCAAGTACTTTGAAACA TCTGCCTTGAAAGAAGAAGCAACAAAAAGCATTGAGCCAGGCAGTGATTACTATGAACTGAGTGACACT AGAGAAAGTGTCCATGAGTCTATTGATACCATGTCTCCCATGCATAAAAATGGTGACAAGGAGTTTCAA ACAGGAAAAGAATCCCAGCCCAGTCCTCCAGCACAAGAAGCAGGGTACAGCACTCTCGCACAGAGTTAT CCATCAGATTTACCTGAAGAACCCAGTTCTCCTCAAGAAAGAATGTTCACTATTGATCCAAAAGTGTAT GGAGAGAAAAGGGACCTCCACAGTAAGAATAAGGATGATTTGACCCTTAGCAGGAGTTTAGGACTTGGT GGTAGGTCTGCAATAGAACAAAGAAGCATGTCAATCAATTTGCCGATGTCTTGCCTAGATTCCATAGCC CTTGGATTTAACTTTGGTCGGGGACATGATCTTTCTCCTCTGGCTTCCGATATTCTAACCAACACTAGT GGAAGTATGGATGAAGGGGATGATTACCTTCCAGCCACCACACCTGCACTGGAGAAAGCCCCTTGCTTC CCTGTAGAAAGCAAAGAGGAAGAACAGATAGAGAAAGTAAAAGCTACTGGAGAAGAAAGTACTCAAGCG GAGATATCATGTGAGTCTCCTTTCCTAGCCAAAGATTTTTACAAAAATGGTACTGTCATGGCACCTGAC CTTCCTGAAATGCTAGATCTGGCAGGCACAAGGTCAAGATTGGCTTCTGTGAGTGCAGATGCTGAGGTT GCCAGGAGGAAATCAGTCCCATCAGAGACTGTGGTTGAGGATAGTCGTACTGGCTTGCCCCCGGTAACT GATGAAAACCATGTCATTGTAAAAACGGACAGTCAGCTCGAAGACCTGGGCTACTGTGTGTTCAATAAG TACACAGTCCCATTGCCATCACCTGTTCAAGACAGTGAGAATTTATCAGGGGAGAGTGGTACCTTTTAC GAAGGCACTGATGATAAAGTTCGAAGAGATTTGGCCACAGACCTTTCACTGATTGAAGTGAAACTGGCA GCAGCCGGAAGAGTCAAAGATGAGTTCAGTGTTGACAAAGAAGCATCCGCGCATATCTCTGGTGACAAA TCAGGACTGAGTAAGGAGTTTGACCAAGAGAAGAAAGCTAATGATAGGTTGGATACTGTACTAGAAAAG AGTGAAGAACATGCTGATTCAAAAGAACATGCCAAGAAAACTGAAGAGGCTGGTGATGAAATAGAAACA TTCGGATTAGGAGTAACCTATGAGCAAGCTTTGGCCAAAGATTTGTCAATACCAACAGATGCATCCTCT GAGAAAGCAGAGAAGGGTCTTAGTTCAGTGCCAGAGATAGCTGAGGTAGAACCATCCAAAAAGGTGGAA CAAGGTCTGGATTTTGCTGTCCAGGGTCAACTAGATGTTAAAATTAGTGACTTTGGACAGATGGCTTCA GGGCTAAACATAGATGATAGAAGGGCAACAGAGCTAAAACTTGAGGCTACACAGGACATGACCCCCTCA TCCAAAGCACCGCAGGAGGCAGATGCATTTATGGGTGTTGAGTCTGGCCACATGAAAGAAGGCACTAAA GTTAGTGAGACAGAAGTCAAAGAGAAGGTGGCCAAGCCTGACTTGGTGCACCAGGAGGCTGTAGACAAG GAGGAGTCCTATGAATCTAGTGGTGAGCATGAAAGTCTCACCATGGAGTCCTTGAAAGCTGATGAGGGC AAGAAGGAAACATCTCCAGAATCATCTCTAATTCAAGATGAGATTGCCGTCAAATTGTCAGTGGAAATA CCTTGCCCACCTGCTGTTTCAGAGGCTGATTTAGCCACAGATGAGAGAGCTGATGTCCAGATGGAATTT ATTCAGGGGCCAAAAGAAGAAAGCAAAGAGACCCCAGATATATCCATCACGCCTTCTGATGTTGCAGAG CCATTGCATGAAACGATCGTATCTGAACCAGCAGAGATTCAGAGTGAGGAAGAAGAGATAGAAGCCCAG GGAGAATATGATAAACTGCTCTTCCGCTCAGACACCCTTCAGATAACTGACCTGGGTGTCTCAGGTGCC AGGGAGGAATTTGTGGAGACCTGCCCAAGTGAACACAAAGGAGTGATTGAGTCTGTTGTGACCATCGAG GATGATTTCATCACTGTAGTGCAAACCACAACTGATGAAGGGGAGTCAGGGTCCCACAGCGTGCGTTTT GCAGCCCTAGAGCAGCCTGAGGTGGAAAGGAGACCATCTCCTCATGATGAAGAAGAGTTTGAAGTAGAA GAGGCAGCTGAAGCCCAGGCAGAACCCAAAGATGGTTCCCCAGAGGCTCCAGCTTCCCCTGAGAGAGAA GAGGTTGCACTTTCTGAATATAAGACAGAAACCTATGACGATTACAAAGATGAGACCACCATTGACGAC TCCATCATGGACGCTGACAGCCTCTGGGTGGACACTCAAGATGATGATAGGAGCATCATGACAGAACAG TTAGAAACTATTCCTAAAGAGGAGAAAGCTGAAAAGGAAGCTCGGAGATCATCTCTTGAGAAACATAGA AAAGAAAAGCCTTTTAAAACCGGGAGAGGCAGAATTTCCACTCCTGAAAGAAAAGTAGCTAAAAAGGAA CCTAGCACAGTCTCCAGAGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCTGAACTTGCTAAA AAAACAGAAGTTCAGGCCCACTCTCCCTCCAGGAAATTCATTTTAAAACCTGCTATCAAATATACTAGA CCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAGCAGGTGGGGAATCAGCTCTGGCTCCCAGT GTATTTAAACAGGCAAAGGACAAAGTCTCTGACGGAGTAACCAAGAGCCCAGAAAAGCGCTCTTCTCTC CCAAGACCTTCCTCCATTCTCCCTCCTCGGCGAGGTGTGTCAGGAGACAGAGATGAGAATTCCTTCTCT CTCAACAGTTCTATCTCTTCTTCAGCACGGCGGACCACCAGGTCAGAGCCAATTCGCAGAGCAGGGAAG AGTGGTACCTCAACACCCACTACCCCTGGGTCTACTGCCATCACTCCTGGCACCCCACCAAGTTATTCT TCACGCACACCAGGCACTCCTGGAACCCCTAGCTATCCCAGGACCCCTCACACACCAGGAACCCCCAAG TCTGCCATCTTGGTGCCGAGTGAGAAGAAGGTCGCCATCATACGTACTCCTCCAAAATCTCCTGCGACT CCCAAGCAGCTTCGGCTTATTAACCAACCACTGCCAGACCTGAAGAATGTCAAATCCAAAATCGGATCA ACAGACAACATCAAATACCAGCCTAAAGGGGGGCAGGTACAAATTGTTACCAAGAAAATAGACCTAAGC CATGTGACATCCAAATGTGGCTCTCTGAAGAACATCCGCCACAGGCCAGGTGGCGGACGTGTGAAAATT GAGAGTGTAAAACTAGATTTCAAAGAAAAGGCCCAAGCTAAAGTTGGTTCTCTTGATAATGCTCATCAT GTACCTGGAGGTGGTAATGTCAAGATTGACAGCCAAAAGTTGAACTTCAGAGAGCATGCTAAAGCCCGT GTGGACCATGGGGCTGAGATCATTACACAGTCCCCAGGCAGATCCAGCGTGGCATCACCCCGACGACTC AGCAATGTCTCCTCGTCTGGAAGCATCAACCTGCTCGAATCTCCTCAGCTTGCCACTTTGGCTGAGGAT GTCACTGCTGCACTCGCTAAGCAGGGCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC216775 Blue=ORF Red=Cloning site Green=Tag(s) MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSN TKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPP PSPASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTY PDKKDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKD EWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFL QPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEA INQETVQQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSE QKDQEPTTDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGA ATSAELDMPFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQ TGKESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLG GRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCF PVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEV ARRKSVPSETVVEDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFY EGTDDKVRRDLATDLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEK SEEHADSKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVE QGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTK VSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEI PCPPAVSEADLATDERADVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQ GEYDKLLFRSDTLQITDLGVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRF AALEQPEVERRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDD SIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKE PSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPS VFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGK SGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPAT PKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKI ESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRL SNVSSSGSINLLESPQLATLAEDVTAALAKQGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC216775 also available, TP316775
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_002374
ORF Size	5481 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq Size	6534 bp
RefSeq ORF	5484 bp
Locus ID	4133
UniProt ID	P11137
Domains	tubulin-binding
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS
MW	199.5 kDa
Gene Summary	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

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