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TAT (NM_000353) Human Tagged ORF Clone
CAT＃: RC216782
TAT (Myc-DDK-tagged)-Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_000353" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216782 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCTCCTCAATTCTGGACGTGCATGTCAACG TTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAGAAAGGCCAGGTGGTCTGTGAGGCCCTCAGA CATGGCCAAGAAAACTTTCAACCCCATCCGAGCCATTGTGGACAACATGAAGGTGAAACCAAATCCAAAC AAAACCATGATTTCCCTGTCCATTGGGGACCCTACTGTGTTTGGAAACCTGCCTACAGACCCTGAAGTTA CCCAGGCAATGAAAGATGCCCTGGACTCGGGCAAATATAATGGCTATGCCCCATCCATCGGCTTCCTATC CAGTCGGGAGGAGATTGCTTCTTATTACCACTGTCCTGAGGCACCCCTAGAAGCTAAGGACGTCATTCTG ACAAGTGGCTGCAGCCAAGCTATTGACCTTTGTTTAGCTGTGTTGGCCAACCCAGGGCAAAACATCCTGG TTCCAAGACCTGGTTTCTCTCTCTACAAGACTCTGGCTGAGTCTATGGGAATTGAGGTCAAACTCTACAA TTTGTTGCCAGAGAAATCTTGGGAAATTGACCTGAAACAACTGGAATATCTAATTGATGAAAAGACAGCT TGTCTCATTGTCAATAATCCATCAAACCCCTGTGGGTCAGTGTTCAGCAAACGTCATCTTCAGAAGATTC TGGCAGTGGCTGCACGGCAGTGTGTCCCCATCTTAGCTGATGAGATCTATGGAGACATGGTGTTTTCGGA TTGCAAATATGAACCACTGGCCACCCTCAGCACCGATGTCCCCATCCTGTCCTGTGGAGGGCTGGCCAAG CGCTGGCTGGTTCCTGGCTGGAGGTTGGGCTGGATCCTCATTCATGACCGAAGAGACATTTTTGGCAATG AGATCCGAGATGGGCTGGTGAAGCTGAGTCAGCGCATTTTGGGACCCTGTACCATTGTCCAGGGAGCTCT GAAAAGCATCCTATGTCGCACCCCGGGAGAGTTTTACCACAACACTCTGAGCTTCCTCAAGTCCAATGCT GATCTCTGTTATGGGGCGTTGGCTGCCATCCCTGGACTCCGGCCAGTCCGCCCTTCTGGGGCTATGTACC TCATGGTTGGAATTGAGATGGAACATTTCCCAGAATTTGAGAACGATGTGGAGTTCACGGAGCGGTTAGT TGCTGAGCAGTCTGTCCACTGCCTCCCAGCAACGTGCTTTGAGTACCCGAATTTCATCCGAGTGGTCATC ACAGTCCCCGAGGTGATGATGCTGGAGGCGTGCAGCCGGATCCAGGAGTTCTGTGAGCAGCACTACCATT GTGCTGAAGGCAGCCAGGAGGAGTGTGATAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216782 protein sequence
Red=Cloning site Green=Tags(s) MDPYMIQMSSKGNLSSILDVHVNVGGRSSVPGKMKGRKARWSVRPSDMAKKTFNPIRAIVDNMKVKPNPN KTMISLSIGDPTVFGNLPTDPEVTQAMKDALDSGKYNGYAPSIGFLSSREEIASYYHCPEAPLEAKDVIL TSGCSQAIDLCLAVLANPGQNILVPRPGFSLYKTLAESMGIEVKLYNLLPEKSWEIDLKQLEYLIDEKTA CLIVNNPSNPCGSVFSKRHLQKILAVAARQCVPILADEIYGDMVFSDCKYEPLATLSTDVPILSCGGLAK RWLVPGWRLGWILIHDRRDIFGNEIRDGLVKLSQRILGPCTIVQGALKSILCRTPGEFYHNTLSFLKSNA DLCYGALAAIPGLRPVRPSGAMYLMVGIEMEHFPEFENDVEFTERLVAEQSVHCLPATCFEYPNFIRVVI TVPEVMMLEACSRIQEFCEQHYHCAEGSQEECDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000353 |
| ORF Size | 1362 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000353.3 |
| RefSeq Size | 2757 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 6898 |
| UniProt ID | P17735 |
| Domains | aminotran_1_2 |
| Protein Families | Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism, Ubiquinone and other terpenoid-quinone biosynthesis |
| MW | 50.4 kDa |
| Gene Summary | This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible cognitive disability. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis
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Bioactive Materials
,PubMed ID 35224289
[TAT]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216782L3 | Lenti ORF clone of Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216782L4 | Lenti ORF clone of Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG216782 | TAT (tGFP-tagged) - Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC119924 | TAT (untagged)-Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
