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CAMK1D (NM_020397) Human Tagged ORF Clone
CAT＃: RC217129
CAMK1D (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 1
ORF Plasmid: tGFP
"NM_020397" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CaM-K1; CaMKID; CKLiK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217129 representing NM_020397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGAGAACGGCGAGAGCAGCTCCTCCTGGAAAAAGCAAGCTGAAGACATCAAGAAGATCTTCG AGTTCAAAGAGACCCTCGGAACCGGGGCCTTTTCCGAAGTGGTTTTAGCTGAAGAGAAGGCAACTGGCAA GCTCTTTGCTGTGAAGTGTATCCCTAAGAAGGCGCTGAAGGGCAAGGAAAGCAGCATAGAGAATGAGATA GCCGTCCTGAGAAAGATTAAGCATGAAAATATTGTTGCCCTGGAAGACATTTATGAAAGCCCAAATCACC TGTACTTGGTCATGCAGCTGGTGTCCGGTGGAGAGCTGTTTGACCGGATAGTGGAGAAGGGGTTTTATAC AGAGAAGGATGCCAGCACTCTGATCCGCCAAGTCTTGGACGCCGTGTACTATCTCCACAGAATGGGCATC GTCCACAGAGACCTCAAGCCCGAAAATCTCTTGTACTACAGTCAAGATGAGGAGTCCAAAATAATGATCA GTGACTTTGGATTGTCAAAAATGGAGGGCAAAGGAGATGTGATGTCCACTGCCTGTGGAACTCCAGGCTA TGTCGCTCCTGAAGTCCTCGCCCAGAAACCTTACAGCAAAGCCGTTGACTGCTGGTCCATCGGAGTGATT GCCTACATCTTGCTCTGCGGCTACCCTCCTTTTTATGATGAAAATGACTCCAAGCTCTTTGAGCAGATCC TCAAGGCGGAATATGAGTTTGACTCTCCCTACTGGGATGACATCTCCGACTCTGCAAAAGACTTCATTCG GAACCTGATGGAGAAGGACCCGAATAAAAGATACACGTGTGAGCAGGCAGCTCGGCACCCATGGATCGCT GGTGACACAGCCCTCAACAAAAACATCCACGAGTCCGTCAGCGCCCAGATCCGGAAAAACTTTGCCAAGA GCAAATGGAGACAAGCATTTAATGCCACGGCCGTCGTCAGACATATGAGAAAACTACACCTCGGCAGCAG CCTGGACAGTTCAAATGCAAGTGTTTCGAGCAGCCTCAGTTTGGCCAGCCAAAAAGACTGTGCGTATGTA GCAAAACCAGAATCCCTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217129 representing NM_020397
Red=Cloning site Green=Tags(s) MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEI AVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGI VHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVI AYILLCGYPPFYDENDSKLFEQILKAEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIA GDTALNKNIHESVSAQIRKNFAKSKWRQAFNATAVVRHMRKLHLGSSLDSSNASVSSSLSLASQKDCAYV AKPESLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020397 |
| ORF Size | 1071 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020397.4 |
| RefSeq Size | 1727 bp |
| RefSeq ORF | 1074 bp |
| Locus ID | 57118 |
| UniProt ID | Q8IU85 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 40 kDa |
| Gene Summary | This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217129L3 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217129L4 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217129 | CAMK1D (tGFP-tagged) - Human calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 1 |
CNY 4,370.00 |
|
| SC313112 | CAMK1D (untagged)-Human calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 1 |
CNY 3,656.00 |
