LOXL3 (NM_032603) Human Tagged ORF Clone

CAT#: RC217575

  • TrueORF®

LOXL3 (Myc-DDK-tagged)-Human lysyl oxidase-like 3 (LOXL3)

ORF Plasmid: DDK tGFP



  "NM_032603" in other vectors (6)

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CNY 5,512.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-LOXL3 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms LOXL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217575 representing NM_032603
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGACCTGTCAGTGTCTGGCAGTGGAGCCCCTGGGGGCTGCTGCTGTGCCTGCTGTGCAGTTCGTGCT
TGGGGTCTCCGTCCCCTTCCACGGGCCCTGAGAAGAAGGCCGGGAGCCAGGGGCTTCGGTTCCGGCTGGC
TGGCTTCCCCAGGAAGCCCTACGAGGGCCGCGTGGAGATACAGCGAGCTGGTGAATGGGGCACCATCTGC
GATGATGACTTCACGCTGCAGGCTGCCCACATCCTCTGCCGGGAGCTGGGCTTCACAGAGGCCACAGGCT
GGACCCACAGTGCCAAATATGGCCCTGGAACAGGCCGCATCTGGCTGGACAACTTGAGCTGCAGTGGGAC
CGAGCAGAGTGTGACTGAATGTGCCTCCCGGGGCTGGGGGAACAGTGACTGTACGCACGATGAGGATGCT
GGGGTCATCTGCAAAGACCAGCGCCTCCCTGGCTTCTCGGACTCCAATGTCATTGAGGTAGAGCATCACC
TGCAAGTGGAGGAGGTGCGAATTCGACCCGCCGTTGGGTGGGGCAGACGACCCCTGCCCGTGACGGAGGG
GCTGGTGGAAGTCAGGCTTCCTGACGGCTGGTCGCAAGTGTGCGACAAAGGCTGGAGCGCCCACAACAGC
CACGTGGTCTGCGGGATGCTGGGCTTCCCCAGCGAAAAGAGGGTCAACGCGGCCTTCTACAGGCTGCTAG
CCCAACGGCAGCAACACTCCTTTGGTCTGCATGGGGTGGCGTGCGTGGGCACGGAGGCCCACCTCTCCCT
CTGTTCCCTGGAGTTCTATCGTGCCAATGACACCGCCAGGTGCCCTGGGGGGGGCCCTGCAGTGGTGAGC
TGTGTGCCAGGCCCTGTCTACGCGGCATCCAGTGGCCAGAAGAAGCAACAACAGTCGAAGCCTCAGGGGG
AGGCCCGTGTCCGTCTAAAGGGCGGCGCCCACCCTGGAGAGGGCCGGGTAGAAGTCCTGAAGGCCAGCAC
ATGGGGCACAGTCTGTGACCGCAAGTGGGACCTGCATGCAGCCAGCGTGGTGTGTCGGGAGCTGGGCTTC
GGGAGTGCTCGAGAAGCTCTGAGTGGCGCTCGCATGGGGCAGGGCATGGGTGCTATCCACCTGAGTGAAG
TTCGCTGCTCTGGACAGGAGCTCTCCCTCTGGAAGTGCCCCCACAAGAACATCACAGCTGAGGATTGTTC
ACATAGCCAGGATGCCGGGGTCCGGTGCAACCTACCTTACACTGGGGCAGAGACCAGGATCCGACTCAGT
GGGGGCCGCAGCCAACATGAGGGGCGAGTCGAGGTGCAAATAGGGGGACCTGGGCCCCTTCGCTGGGGCC
TCATCTGTGGGGATGACTGGGGGACCCTGGAGGCCATGGTGGCCTGTAGGCAACTGGGTCTGGGCTACGC
CAACCACGGCCTGCAGGAGACCTGGTACTGGGACTCTGGGAATATAACAGAGGTGGTGATGAGTGGAGTG
CGCTGCACAGGGACTGAGCTGTCCCTGGATCAGTGTGCCCATCATGGCACCCACATCACCTGCAAGAGGA
CAGGGACCCGCTTCACTGCTGGAGTCATCTGTTCTGAGACTGCATCAGATCTGTTGCTGCACTCAGCACT
GGTGCAGGAGACCGCCTACATCGAAGACCGGCCCCTGCATATGTTGTACTGTGCTGCGGAAGAGAACTGC
CTGGCCAGCTCAGCCCGCTCAGCCAACTGGCCCTATGGTCACCGGCGTCTGCTCCGATTCTCCTCCCAGA
TCCACAACCTGGGACGAGCTGACTTCAGGCCCAAGGCTGGGCGCCACTCCTGGGTGTGGCACGAGTGCCA
TGGGCATTACCACAGCATGGACATCTTCACTCACTATGATATCCTCACCCCAAATGGCACCAAGGTGGCT
GAGGGCCACAAAGCTAGTTTCTGTCTCGAAGACACTGAGTGTCAGGAGGATGTCTCCAAGCGGTATGAGT
GTGCCAACTTTGGAGAGCAAGGCATCACTGTGGGTTGCTGGGATCTCTACCGGCATGACATTGACTGTCA
GTGGATTGACATCACGGATGTGAAGCCAGGAAACTACATTCTCCAGGTTGTCATCAACCCAAACTTTGAA
GTAGCAGAGAGTGACTTTACCAACAATGCAATGAAATGTAACTGCAAATATGATGGACATAGAATCTGGG
TGCACAACTGCCACATTGGTGATGCCTTCAGTGAAGAGGCCAACAGGAGGTTTGAACGCTACCCTGGCCA
GACCAGCAACCAGATTATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217575 representing NM_032603
Red=Cloning site Green=Tags(s)

MRPVSVWQWSPWGLLLCLLCSSCLGSPSPSTGPEKKAGSQGLRFRLAGFPRKPYEGRVEIQRAGEWGTIC
DDDFTLQAAHILCRELGFTEATGWTHSAKYGPGTGRIWLDNLSCSGTEQSVTECASRGWGNSDCTHDEDA
GVICKDQRLPGFSDSNVIEVEHHLQVEEVRIRPAVGWGRRPLPVTEGLVEVRLPDGWSQVCDKGWSAHNS
HVVCGMLGFPSEKRVNAAFYRLLAQRQQHSFGLHGVACVGTEAHLSLCSLEFYRANDTARCPGGGPAVVS
CVPGPVYAASSGQKKQQQSKPQGEARVRLKGGAHPGEGRVEVLKASTWGTVCDRKWDLHAASVVCRELGF
GSAREALSGARMGQGMGAIHLSEVRCSGQELSLWKCPHKNITAEDCSHSQDAGVRCNLPYTGAETRIRLS
GGRSQHEGRVEVQIGGPGPLRWGLICGDDWGTLEAMVACRQLGLGYANHGLQETWYWDSGNITEVVMSGV
RCTGTELSLDQCAHHGTHITCKRTGTRFTAGVICSETASDLLLHSALVQETAYIEDRPLHMLYCAAEENC
LASSARSANWPYGHRRLLRFSSQIHNLGRADFRPKAGRHSWVWHECHGHYHSMDIFTHYDILTPNGTKVA
EGHKASFCLEDTECQEDVSKRYECANFGEQGITVGCWDLYRHDIDCQWIDITDVKPGNYILQVVINPNFE
VAESDFTNNAMKCNCKYDGHRIWVHNCHIGDAFSEEANRRFERYPGQTSNQII

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032603
ORF Size 2259 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032603.5
RefSeq Size 3121 bp
RefSeq ORF 2262 bp
Locus ID 84695
UniProt ID P58215
Protein Families Druggable Genome
MW 80.3 kDa
Gene Summary This gene encodes a lysyl oxidase, which likely functions as an amine oxidase and plays a role in the formation of crosslinks in collagens and elastin. Deletion of the related gene in mouse causes neonatal mortality with cleft palate, spine deformity, and defects in collagen organization. A mutation in this gene was found in a family with Stickler syndrome. [provided by RefSeq, Sep 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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