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VR1 (TRPV1) (NM_080705) Human Tagged ORF Clone
CAT＃: RC217713
TRPV1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4
ORF Plasmid: tGFP
"NM_080705" in other vectors (6)
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CNY 9,216.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | VR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217713 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAATGGAGCAGCACAGACTTGGGGGCAGCTGCGGACCCACTCCAAAAGGACACCTGCCCAGACC CCCTGGATGGAGACCCTAACTCCAGGCCACCTCCAGCCAAGCCCCAGCTCTCCACGGCCAAGAGCCGCAC CCGGCTCTTTGGGAAGGGTGACTCGGAGGAGGCTTTCCCGGTGGATTGCCCTCACGAGGAAGGTGAGCTG GACTCCTGCCCGACCATCACAGTCAGCCCTGTTATCACCATCCAGAGGCCAGGAGACGGCCCCACCGGTG CCAGGCTGCTGTCCCAGGACTCTGTCGCCGCCAGCACCGAGAAGACCCTCAGGCTCTATGATCGCAGGAG TATCTTTGAAGCCGTTGCTCAGAATAACTGCCAGGATCTGGAGAGCCTGCTGCTCTTCCTGCAGAAGAGC AAGAAGCACCTCACAGACAACGAGTTCAAAGACCCTGAGACAGGGAAGACCTGTCTGCTGAAAGCCATGC TCAACCTGCACGACGGACAGAACACCACCATCCCCCTGCTCCTGGAGATCGCGCGGCAAACGGACAGCCT GAAGGAGCTTGTCAACGCCAGCTACACGGACAGCTACTACAAGGGCCAGACAGCACTGCACATCGCCATC GAGAGACGCAACATGGCCCTGGTGACCCTCCTGGTGGAGAACGGAGCAGACGTCCAGGCTGCGGCCCATG GGGACTTCTTTAAGAAAACCAAAGGGCGGCCTGGATTCTACTTCGGTGAACTGCCCCTGTCCCTGGCCGC GTGCACCAACCAGCTGGGCATCGTGAAGTTCCTGCTGCAGAACTCCTGGCAGACGGCCGACATCAGCGCC AGGGACTCGGTGGGCAACACGGTGCTGCACGCCCTGGTGGAGGTGGCCGACAACACGGCCGACAACACGA AGTTTGTGACGAGCATGTACAATGAGATTCTGATCCTGGGGGCCAAACTGCACCCGACGCTGAAGCTGGA GGAGCTCACCAACAAGAAGGGAATGACGCCGCTGGCTCTGGCAGCTGGGACCGGGAAGATCGGGGTCTTG GCCTATATTCTCCAGCGGGAGATCCAGGAGCCCGAGTGCAGGCACCTGTCCAGGAAGTTCACCGAGTGGG CCTACGGGCCCGTGCACTCCTCGCTGTACGACCTGTCCTGCATCGACACCTGCGAGAAGAACTCGGTGCT GGAGGTGATCGCCTACAGCAGCAGCGAGACCCCTAATCGCCACGACATGCTCTTGGTGGAGCCGCTGAAC CGACTCCTGCAGGACAAGTGGGACAGATTCGTCAAGCGCATCTTCTACTTCAACTTCCTGGTCTACTGCC TGTACATGATCATCTTCACCATGGCTGCCTACTACAGGCCCGTGGATGGCTTGCCTCCCTTTAAGATGGA AAAAACTGGAGACTATTTCCGAGTTACTGGAGAGATCCTGTCTGTGTTAGGAGGAGTCTACTTCTTTTTC CGAGGGATTCAGTATTTCCTGCAGAGGCGGCCGTCGATGAAGACCCTGTTTGTGGACAGCTACAGTGAGA TGCTTTTCTTTCTGCAGTCACTGTTCATGCTGGCCACCGTGGTGCTGTACTTCAGCCACCTCAAGGAGTA TGTGGCTTCCATGGTATTCTCCCTGGCCTTGGGCTGGACCAACATGCTCTACTACACCCGCGGTTTCCAG CAGATGGGCATCTATGCCGTCATGATAGAGAAGATGATCCTGAGAGACCTGTGCCGTTTCATGTTTGTCT ACATCGTCTTCTTGTTCGGGTTTTCCACAGCGGTGGTGACGCTGATTGAAGACGGGAAGAATGACTCCCT GCCGTCTGAGTCCACGTCGCACAGGTGGCGGGGGCCTGCCTGCAGGCCCCCCGATAGCTCCTACAACAGC CTGTACTCCACCTGCCTGGAGCTGTTCAAGTTCACCATCGGCATGGGCGACCTGGAGTTCACTGAGAACT ATGACTTCAAGGCTGTCTTCATCATCCTGCTGCTGGCCTATGTAATTCTCACCTACATCCTCCTGCTCAA CATGCTCATCGCCCTCATGGGTGAGACTGTCAACAAGATCGCACAGGAGAGCAAGAACATCTGGAAGCTG CAGAGAGCCATCACCATCCTGGACACGGAGAAGAGCTTCCTTAAGTGCATGAGGAAGGCCTTCCGCTCAG GCAAGCTGCTGCAGGTGGGGTACACACCTGATGGCAAGGACGACTACCGGTGGTGCTTCAGGGTGGACGA GGTGAACTGGACCACCTGGAACACCAACGTGGGCATCATCAACGAAGACCCGGGCAACTGTGAGGGCGTC AAGCGCACCCTGAGCTTCTCCCTGCGGTCAAGCAGAGTTTCAGGCAGACACTGGAAGAACTTTGCCCTGG TCCCCCTTTTAAGAGAGGCAAGTGCTCGAGATAGGCAGTCTGCTCAGCCCGAGGAAGTTTATCTGCGACA GTTTTCAGGGTCTCTGAAGCCAGAGGACGCTGAGGTCTTCAAGAGTCCTGCCGCTTCCGGGGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217713 protein sequence
Red=Cloning site Green=Tags(s) MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGEL DSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKS KKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQTALHIAI ERRNMALVTLLVENGADVQAAAHGDFFKKTKGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISA RDSVGNTVLHALVEVADNTADNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVL AYILQREIQEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN RLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKTGDYFRVTGEILSVLGGVYFFF RGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVASMVFSLALGWTNMLYYTRGFQ QMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLIEDGKNDSLPSESTSHRWRGPACRPPDSSYNS LYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKL QRAITILDTEKSFLKCMRKAFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGV KRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080705 |
| ORF Size | 2517 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080705.4 |
| RefSeq Size | 4117 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 7442 |
| UniProt ID | Q8NER1 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 94.9 kDa |
| Gene Summary | Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Contact sensitizer 2,4-dinitrochlorobenzene is a highly potent human TRPA1 agonist
,Saarnilehto, M;Chapman, H;Savinko, T;Lindstedt, K;Lauerma, AI;Koivisto, A;,
Allergy July 2014
,PubMed ID 25041656
[VR1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217713L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4, Myc-DDK-tagged |
CNY 11,616.00 |
|
| RC217713L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4, mGFP tagged |
CNY 11,616.00 |
|
| RC217713L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217713L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RG217713 | TRPV1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4 |
CNY 10,816.00 |
|
| SC305819 | TRPV1 (untagged)-Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4 |
CNY 9,224.00 |
