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Cytohesin 2 (CYTH2) (NM_017457) Human Tagged ORF Clone
CAT＃: RC217980
CYTH2 (Myc-DDK-tagged)-Human cytohesin 2 (CYTH2), transcript variant 1
ORF Plasmid: tGFP
"NM_017457" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARNO; CTS18; CTS18.1; cytohesin-2; PSCD2; PSCD2L; SEC7L; Sec7p-L; Sec7p-like |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217980 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGCGTCTATGAACCCCCAGACCTGACTCCGGAGGAGCGGATGGAGCTGGAGAACATCCGGC GGCGGAAGCAGGAGCTGCTGGTGGAGATTCAGCGCCTGCGGGAGGAGCTCAGTGAAGCCATGAGCGAGGT GGAGGGGCTGGAGGCCAATGAGGGCAGTAAGACCTTGCAACGGAACCGGAAGATGGCAATGGGCAGGAAG AAGTTCAACATGGACCCCAAGAAGGGGATCCAGTTCTTGGTGGAGAATGAACTGCTGCAGAACACACCCG AGGAGATCGCCCGCTTCCTGTACAAGGGCGAGGGGCTGAACAAGACAGCCATCGGGGACTACCTGGGGGA GAGGGAAGAACTGAACCTGGCAGTGCTCCATGCTTTTGTGGATCTGCATGAGTTCACCGACCTCAATCTG GTGCAGGCCCTCAGGCAGTTTCTATGGAGCTTTCGCCTACCCGGAGAGGCCCAGAAAATTGACCGGATGA TGGAGGCCTTCGCCCAGCGATACTGCCTGTGCAACCCTGGGGTTTTCCAGTCCACAGACACGTGCTATGT GCTGTCCTTCGCCGTCATCATGCTCAACACCAGTCTCCACAATCCCAATGTCCGGGACAAGCCGGGCCTG GAGCGCTTTGTGGCCATGAACCGGGGCATCAACGAGGGCGGGGACCTGCCTGAGGAGCTGCTCAGGAACC TGTACGACAGCATCCGAAATGAGCCCTTCAAGATTCCTGAGGATGACGGGAATGACCTGACCCACACCTT CTTCAACCCGGACCGGGAGGGCTGGCTCCTGAAGCTGGGAGGGGGCCGGGTGAAGACGTGGAAGCGGCGC TGGTTTATCCTCACAGACAACTGCCTCTACTACTTTGAGTACACCACGGACAAGGAGCCCCGAGGAATCA TCCCCCAGGAGAATCTGAGCATCCGAGAGGTGGACGACCCCCGGAAACCGAACTGCTTTGAACTTTACAT CCCCAACAACAAGGGGCAGCTCATCAAAGCCTGCAAAACTGAGGCGGACGGCCGAGTGGTGGAGGGAAAC CACATGGTGTACCGGATCTCGGCCCCCACGCAGGAGGAGAAGGACGAGTGGATCAAGTCCATCCAGGCGG CTGTGAGTGTGGACCCCTTCTATGAGATGCTGGCAGCGAGAAAGAAGCGGATTTCAGTCAAGAAGAAGCA GGAGCAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217980 protein sequence
Red=Cloning site Green=Tags(s) MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRK KFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNL VQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGL ERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRR WFILTDNCLYYFEYTTDKEPRGIIPQENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGN HMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017457 |
| ORF Size | 1200 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017457.5 |
| RefSeq Size | 4625 bp |
| RefSeq ORF | 1203 bp |
| Locus ID | 9266 |
| UniProt ID | Q99418 |
| Domains | Sec7, PH |
| Protein Families | Druggable Genome |
| MW | 46.6 kDa |
| Gene Summary | The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217980L1 | Lenti ORF clone of Human cytohesin 2 (CYTH2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC217980L2 | Lenti ORF clone of Human cytohesin 2 (CYTH2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217980L3 | Lenti ORF clone of Human cytohesin 2 (CYTH2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217980L4 | Lenti ORF clone of Human cytohesin 2 (CYTH2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217980 | CYTH2 (tGFP-tagged) - Human cytohesin 2 (CYTH2), transcript variant 1 |
CNY 5,256.00 |
|
| SC114117 | CYTH2 (untagged)-Human cytohesin 2 (CYTH2), transcript variant 1 |
CNY 3,656.00 |
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