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SPON1 (NM_006108) Human Tagged ORF Clone
CAT＃: RC218131
SPON1 (Myc-DDK-tagged)-Human spondin 1, extracellular matrix protein (SPON1)
ORF Plasmid: tGFP
"NM_006108" in other vectors (6)
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CNY 5,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | f-spondin; VSGP/F-spondin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218131 representing NM_006108
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGTCCCCGGCGCCCCTGAAGCTGAGCCGGACTCCGGCACTGCTGGCCCTGGCGCTGCCCCTGG CCGCGGCGCTGGCCTTCTCCGACGAGACCCTGGACAAAGTGCCCAAGTCAGAGGGCTACTGCAGCCGTAT CCTGCGCGCCCAGGGCACGCGGCGCGAGGGCTACACCGAGTTCAGCCTCCGCGTGGAGGGCGACCCCGAC TTCTACAAGCCGGGAACCAGCTACCGCGTAACACTTTCAGCTGCTCCTCCCTCCTACTTCAGAGGATTCA CATTAATTGCCCTCAGAGAGAACAGAGAGGGTGATAAGGAAGAAGACCATGCTGGGACCTTCCAGATCAT AGACGAAGAAGAAACTCAGTTTATGAGCAATTGCCCTGTTGCAGTCACTGAAAGCACTCCACGGAGGAGG ACCCGGATCCAGGTGTTTTGGATAGCACCACCAGCGGGAACAGGCTGCGTGATTCTGAAGGCCAGCATCG TACAAAAACGCATTATTTATTTTCAAGATGAGGGCTCTCTGACCAAGAAACTTTGTGAACAAGATTCCAC ATTTGATGGGGTGACTGACAAACCCATCTTAGACTGCTGTGCCTGCGGAACTGCCAAGTACAGACTCACA TTTTATGGGAATTGGTCCGAGAAGACACACCCAAAGGATTACCCTCGTCGGGCCAACCACTGGTCTGCGA TCATCGGAGGATCCCACTCCAAGAATTATGTACTGTGGGAATATGGAGGATATGCCAGCGAAGGCGTCAA ACAAGTTGCAGAATTGGGCTCACCCGTGAAAATGGAGGAAGAAATTCGACAACAGAGTGATGAGGTCCTC ACCGTCATCAAAGCCAAAGCCCAGTGGCCAGCCTGGCAGCCTCTCAACGTGAGAGCAGCACCTTCAGCTG AATTTTCCGTGGACAGAACGCGCCATTTAATGTCCTTCCTGACCATGATGGGCCCTAGTCCCGACTGGAA CGTAGGCTTATCTGCAGAAGATCTGTGCACCAAGGAATGTGGCTGGGTCCAGAAGGTGGTGCAAGACCTG ATTCCCTGGGACGCTGGCACCGACAGCGGGGTGACCTATGAGTCACCCAACAAACCCACCATTCCCCAGG AGAAAATCCGGCCCCTGACCAGCCTGGACCATCCTCAGAGTCCTTTCTATGACCCAGAGGGTGGGTCCAT CACTCAAGTAGCCAGAGTTGTCATCGAGAGAATCGCACGGAAGGGTGAACAATGCAATATTGTACCTGAC AATGTCGATGATATTGTAGCTGACCTGGCTCCAGAAGAGAAAGATGAAGATGACACCCCTGAAACCTGCA TCTACTCCAACTGGTCCCCATGGTCCGCCTGCAGCTCCTCCACCTGTGACAAAGGCAAGAGGATGCGACA GCGCATGCTGAAAGCACAGCTGGACCTCAGCGTCCCCTGCCCTGACACCCAGGACTTCCAGCCCTGCATG GGCCCTGGCTGCAGTGACGAAGACGGCCCCACCTGCACCATGTCCGAGTGGATCACCTGGTCGCCCTGCA GCATCTCCTGCGGCATGGGCATGAGGTCCCGGGAGAGGTATGTGAAGCAGTTCCCGGAGGACGGCTCCGT GTGCACGCTGCCCACTGAGGAAACGGAGAAGTGCACGGTCAACGAGGAGTGCTCTCCCAGCAGCTGCCTG ATGACCGAGTGGGGCGAGTGGGACGAGTACAGCGCCACCTGCGGCATGGGCATGAAGAAGCGGCACCGCA TGATCAAGATGAACCCCGCAGATGGCTCCATGTGCAAAGCCGAGACATCACAGGCAGAGAAGCGCATGAT GCCAGAGTGCCACACCATCCCATGCTTGCTGTCCCCATGGTCCGAGTGGAGTGACTGCAGCGTGACCTGC GGGAAGGGCATGCGAACCCGACAGCGGATGCTCAAGTCTCTGGCAGAACTTGGAGACTGCAATGAGGATC TGGAGCAGGTGGAGAAGTGCATGCTCCCTGAATGCCCCATTGACTGTGAGCTCACCGAGTGGTCCCAGTG GTCGGAATGTAACAAGTCATGTGGGAAAGGCCACGTGATTCGAACCCGGATGATCCAAATGGAGCCTCAG TTTGGAGGTGCACCCTGCCCAGAGACTGTGCAGCGAAAAAAGTGCCGCATCCGAAAATGCCTTCGAAATC CATCCATCCAAAAGCTACGCTGGAGGGAGGCCCGAGAGAGCCGGCGGAGTGAGCAGCTGAAGGAAGAGTC TGAAGGGGAGCAGTTCCCAGGTTGTAGGATGCGCCCATGGACGGCCTGGTCAGAATGCACCAAACTGTGC GGAGGTGGAATTCAGGAACGTTACATGACTGTAAAGAAGAGATTCAAAAGCTCCCAGTTTACCAGCTGCA AAGACAAGAAGGAGATCAGAGCATGCAATGTTCATCCTTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218131 representing NM_006108
Red=Cloning site Green=Tags(s) MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYTEFSLRVEGDPD FYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRR TRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGTAKYRLT FYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVL TVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDL IPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPD NVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM GPGCSDEDGPTCTMSEWITWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEECSPSSCL MTEWGEWDEYSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKRMMPECHTIPCLLSPWSEWSDCSVTC GKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNKSCGKGHVIRTRMIQMEPQ FGGAPCPETVQRKKCRIRKCLRNPSIQKLRWREARESRRSEQLKEESEGEQFPGCRMRPWTAWSECTKLC GGGIQERYMTVKKRFKSSQFTSCKDKKEIRACNVHPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006108 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006108.1, NP_006099.1 |
| RefSeq Size | 4755 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 10418 |
| UniProt ID | Q9HCB6 |
| Domains | tsp_1, Reeler |
| Protein Families | Secreted Protein |
| MW | 90.8 kDa |
| Gene Summary | Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218131L1 | Lenti ORF clone of Human spondin 1, extracellular matrix protein (SPON1), Myc-DDK-tagged |
CNY 8,312.00 |
|
| RC218131L2 | Lenti ORF clone of Human spondin 1, extracellular matrix protein (SPON1), mGFP tagged |
CNY 5,890.00 |
|
| RC218131L3 | Lenti ORF clone of Human spondin 1, extracellular matrix protein (SPON1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218131L4 | Lenti ORF clone of Human spondin 1, extracellular matrix protein (SPON1), mGFP tagged |
CNY 5,890.00 |
|
| RG218131 | SPON1 (tGFP-tagged) - Human spondin 1, extracellular matrix protein (SPON1) |
CNY 7,512.00 |
|
| SC101178 | SPON1 (untagged)-Human spondin 1, extracellular matrix protein (SPON1) |
CNY 5,920.00 |
