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USH1C (NM_153676) Human Tagged ORF Clone
CAT＃: RC218335
USH1C (Myc-DDK-tagged)-Human Usher syndrome 1C (autosomal recessive, severe) (USH1C), transcript variant b3
ORF Plasmid: tGFP
"NM_153676" in other vectors (4)
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CNY 6,584.00
CNY 9,500.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AIE-75; DFNB18; DFNB18A; NY-CO-37; NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C; ush1cpst |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218335 representing NM_153676
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGAAAAGTGGCCCGAGAATTCCGGCATAAGGTGGATTTTCTGATTGAAAATGATGCAGAGAAGG ACTATCTCTATGATGTGCTGCGAATGTACCACCAGACCATGGACGTGGCCGTGCTCGTGGGAGACCTGAA GCTGGTCATCAATGAACCCAGCCGTCTGCCTCTGTTTGATGCCATTCGGCCGCTGATCCCACTGAAGCAC CAGGTGGAATATGATCAGCTGACCCCCCGGCGCTCCAGGAAGCTGAAGGAGGTGCGTCTGGACCGTCTGC ACCCCGAAGGCCTCGGCCTGAGTGTGCGTGGTGGCCTGGAGTTTGGCTGTGGGCTCTTCATCTCCCACCT CATCAAAGGCGGTCAGGCAGACAGCGTCGGGCTCCAGGTAGGGGACGAGATCGTCCGGATCAATGGATAT TCCATCTCCTCCTGTACCCATGAGGAGGTCATCAACCTCATTCGAACCAAGAAAACTGTGTCCATCAAAG TGAGACACATCGGCCTGATCCCCGTGAAAAGCTCTCCTGATGAGCCCCTCACTTGGCAGTATGTGGATCA GTTTGTGTCGGAATCTGGGGGCGTGCGAGGCAGCCTGGGCTCCCCTGGAAATCGGGAAAACAAGGAGAAG AAGGTCTTCATCAGCCTGGTAGGCTCCCGAGGCCTTGGCTGCAGCATTTCCAGCGGCCCCATCCAGAAGC CTGGCATCTTTATCAGCCATGTGAAACCTGGCTCCCTGTCTGCTGAGGTGGGATTGGAGATAGGGGACCA GATTGTCGAAGTCAATGGCGTCGACTTCTCTAACCTGGATCACAAGGAGGCTGTAAATGTGCTGAAGAGT AGCCGCAGCCTGACCATCTCCATTGTAGCTGCAGCTGGCCGGGAGCTGTTCATGACAGACCGGGAGCGGC TGGCAGAGGCGCGGCAGCGTGAGCTGCAGCGGCAGGAGCTTCTCATGCAGAAGCGGCTGGCGATGGAGTC CAACAAGATCCTCCAGGAGCAGCAGGAGATGGAGCGGCAAAGGAGAAAAGAAATTGCCCAGAAGGCAGCA GAGGAAAATGAGAGATACCGGAAGGAGATGGAACAGATTGTAGAGGAGGAAGAGAAGTTTAAGAAGCAAT GGGAAGAAGACTGGGGCTCAAAGGAACAGCTACTCTTGCCTAAAACCATCACTGCTGAGGTACACCCAGT ACCCCTTCGCAAGCCAAAGTCTTTTGGATGGTTTTATCGTTACGATGGCAAATTCCCAACCATCCGGAAG AAAGGAAAAGATAAGAAGAAAGCCAAGTATGGCAGCCTGCAGGACTTGAGAAAGAATAAGAAAGAACTGG AGTTTGAGCAAAAGCTTTACAAAGAGAAAGAGGAAATGCTGGAGAAGGAAAAGCAGCTAAAGATCAACCG GCTGGCCCAGGAGGTGTCTGAGACAGAGCGGGAAGACCTTGAAGAATCGGAAAAGATTCAATATTGGGTG GAGAGGCTCTGTCAAACGCGCCTCGAGCAGATTTCCTCTGCTGATAATGAGATTTCAGAGATGACCACAG GGCCCCCGCCTCCCCCGCCTTCTGTGTCTCCCCTGGCCCCACCCTTGAGACGCTTCGCAGGCGGACTGCA CCTGCACACCACTGACCTGGACGACATCCCTTTGGACATGTTCTACTATCCCCCCAAGACTCCCTCTGCC TTGCCTGTGATGCCCCACCCTCCACCCTCCAACCCACCCCACAAGGTCCCGGCGCCCCCTGTCCTTCCCT TATCTGGCCATGTGAGCGCCTCATCCTCTCCATGGGTGCAGCGCACTCCACCCCCCATTCCCATCCCTCC CCCGCCATCCGTTCCCACCCAAGACCTCACTCCCACCCGCCCACTGCCCTCGGCGCTGGAAGAAGCACTG AGCAACCATCCCTTCCGCACTGGGGACACAGGCAATCCAGTGGAGGACTGGGAGGCAAAGAACCACAGTG GGAAGCCCACTAACTCCCCTGTCCCTGAACAGAGCTTCCCACCCACCCCAAAGACATTTTGCCCAAGCCC ACAGCCTCCACGAGGCCCTGGCGTGTCCACCATCTCCAAACCTGTCATGGTCCACCAGGAGCCCAATTTC ATCTACAGGCCAGCTGTGAAATCTGAAGTTCTGCCACAGGAGATGTTGAAGAGGATGGTGGTTTATCAGA CAGCATTCAGACAAGATTTCCGGAAATATGAGGAAGGCTTTGACCCCTACTCTATGTTCACCCCAGAGCA GATCATGGGGAAGGATGTCCGGCTCCTACGCATCAAGAAGGAGGGATCCTTAGACCTGGCCCTGGAAGGC GGTGTGGACTCCCCCATTGGGAAGGTGGTCGTTTCTGCTGTGTATGAGCGGGGAGCTGCTGAGCGGCATG GTGGCATTGTGAAAGGGGACGAGATCATGGCAATCAACGGCAAGATTGTGACAGACTACACCCTGGCTGA GGCTGAGGCTGCCCTGCAGAAGGCCTGGAATCAGGGCGGGGACTGGATCGACCTTGTGGTTGCCGTCTGC CCCCCAAAGGAGTATGACGATGAGCTAGCTTCTCTTCCCTCCTCCGTAGCTGAAAGCCCCCAACCGGTCC GAAAGCTCCTTGAAGACCGTGCTGCCGTGCACAGACACGGGTTCCTCCTGCAGCTGGAGCCCACGGACCT TCTTCTGAAGTCCAAAAGGGGAAACCAAATTCACCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218335 representing NM_153676
Red=Cloning site Green=Tags(s) MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKH QVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGY SISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEK KVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEAVNVLKS SRSLTISIVAAAGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAA EENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKSFGWFYRYDGKFPTIRK KGKDKKKAKYGSLQDLRKNKKELEFEQKLYKEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKIQYWV ERLCQTRLEQISSADNEISEMTTGPPPPPPSVSPLAPPLRRFAGGLHLHTTDLDDIPLDMFYYPPKTPSA LPVMPHPPPSNPPHKVPAPPVLPLSGHVSASSSPWVQRTPPPIPIPPPPSVPTQDLTPTRPLPSALEEAL SNHPFRTGDTGNPVEDWEAKNHSGKPTNSPVPEQSFPPTPKTFCPSPQPPRGPGVSTISKPVMVHQEPNF IYRPAVKSEVLPQEMLKRMVVYQTAFRQDFRKYEEGFDPYSMFTPEQIMGKDVRLLRIKKEGSLDLALEG GVDSPIGKVVVSAVYERGAAERHGGIVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVC PPKEYDDELASLPSSVAESPQPVRKLLEDRAAVHRHGFLLQLEPTDLLLKSKRGNQIHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153676 |
| ORF Size | 2697 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153676.4 |
| RefSeq Size | 3250 bp |
| RefSeq ORF | 2700 bp |
| Locus ID | 10083 |
| UniProt ID | Q9Y6N9 |
| MW | 101.2 kDa |
| Gene Summary | This gene encodes a scaffold protein that functions in the assembly of Usher protein complexes. The protein contains PDZ domains, a coiled-coil region with a bipartite nuclear localization signal and a PEST degradation sequence. Defects in this gene are the cause of Usher syndrome type 1C and non-syndromic sensorineural deafness autosomal recessive type 18. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218335L3 | Lenti ORF clone of Human Usher syndrome 1C (autosomal recessive, severe) (USH1C), transcript variant b3, Myc-DDK-tagged |
CNY 11,400.00 |
|
| RC218335L4 | Lenti ORF clone of Human Usher syndrome 1C (autosomal recessive, severe) (USH1C), transcript variant b3, mGFP tagged |
CNY 11,400.00 |
|
| RG218335 | USH1C (tGFP-tagged) - Human Usher syndrome 1C (autosomal recessive, severe) (USH1C), transcript variant b3 |
CNY 10,450.00 |
|
| SC306638 | USH1C (untagged)-Human Usher syndrome 1C (autosomal recessive, severe) (USH1C), transcript variant b3 |
CNY 6,592.00 |
