United States
Germany
Japan
United Kingdom
China
Myosin 1G (MYO1G) (NM_033054) Human Tagged ORF Clone
CAT＃: RC218428
MYO1G (Myc-DDK-tagged)-Human myosin IG (MYO1G)
ORF Plasmid: tGFP
"NM_033054" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HA2; HLA-HA2; MHAG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218428 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGAGGAAGGCCCTGAGTATGGCAAACCTGACTTTGTGCTTTTGGACCAAGTGACCATGGAGG ACTTCATGAGGAACCTGCAGCTCAGGTTCGAGAAGGGCCGCATCTACACCTACATCGGTGAGGTGCTGGT GTCCGTGAACCCCTACCAGGAGCTGCCCCTGTATGGGCCTGAGGCCATCGCCAGGTACCAGGGCCGTGAG CTCTATGAGCGGCCACCCCATCTCTATGCTGTGGCCAACGCCGCCTACAAGGCAATGAAGCACCGGTCCA GGGACACCTGCATCGTCATCTCAGGGGAGAGTGGGGCAGGGAAGACAGAAGCCAGTAAGCACATCATGCA GTACATCGCTGCTGTCACCAATCCAAGCCAGAGGGCTGAGGTGGAGAGGGTCAAGGACGTGCTGCTCAAG TCCACCTGTGTGCTGGAGGCCTTTGGCAATGCCCGCACCAACCGCAATCACAACTCCAGCCGCTTTGGCA AGTACATGGACATCAACTTTGACTTCAAGGGGGACCCGATCGGAGGACACATCCACAGCTACCTACTGGA GAAGTCTCGGGTCCTCAAGCAGCACGTGGGTGAAAGAAACTTCCACGCCTTCTACCAATTGCTGAGAGGC AGTGAGGACAAGCAGCTGCATGAACTGCACTTGGAGAGAAACCCTGCTGTATACAATTTCACACACCAGG GAGCAGGACTCAACATGACTGTGCACAGTGCCTTGGACAGTGATGAGCAGAGCCACCAGGCAGTGACCGA GGCCATGAGGGTCATCGGCTTCAGTCCTGAAGAGGTGGAGTCTGTGCATCGCATCCTGGCTGCCATATTG CACCTGGGAAACATCGAGTTTGTGGAGACGGAGGAGGGTGGGCTGCAGAAGGAGGGCCTGGCAGTGGCCG AGGAGGCACTGGTGGACCATGTGGCTGAGCTGACGGCCACACCCCGGGACCTCGTGCTCCGCTCCCTGCT GGCTCGCACAGTTGCCTCGGGAGGCAGGGAACTCATAGAGAAGGGCCACACTGCAGCTGAGGCCAGCTAT GCCCGGGATGCCTGTGCCAAGGCAGTGTACCAGCGGCTGTTTGAGTGGGTGGTGAACAGGATCAACAGTG TCATGGAACCCCGGGGCCGGGATCCTCGGCGTGATGGCAAGGACACAGTCATTGGCGTGCTGGACATCTA TGGCTTCGAGGTGTTTCCCGTCAACAGTTTCGAGCAGTTCTGCATCAACTACTGCAATGAGAAGCTGCAG CAGCTATTCATCCAGCTCATCCTGAAGCAGGAACAGGAAGAGTACGAGCGCGAGGGCATCACCTGGCAGA GCGTTGAGTATTTCAACAACGCCACCATTGTGGATCTGGTGGAGCGGCCCCACCGTGGCATCCTGGCCGT GCTGGACGAGGCCTGCAGCTCTGCTGGCACCATCACTGACCGAATCTTCCTGCAGACCCTGGACACGCAC CACCGCCATCATCTACACTACACCAGCCGCCAGCTCTGCCCCACAGACAAGACCATGGAGTTTGGCCGAG ACTTCCGGATCAAGCACTATGCAGGGGACGTCACGTACTCCGTGGAAGGCTTCATCGACAAGAACAGAGA TTTCCTCTTCCAGGACTTCAAGCGGCTGCTGTACAACAGCACGGACCCCACTCTACGGGCCATGTGGCCG GACGGGCAGCAGGACATCACAGAGGTGACCAAGCGCCCCCTGACGGCTGGCACACTCTTCAAGAACTCCA TGGTGGCCCTGGTGGAGAACCTTGCCTCCAAGGAGCCCTTCTACGTCCGCTGCATCAAGCCCAATGAGGA CAAGGTAGCTGGGAAGCTGGATGAGAACCACTGTCGCCACCAGGTCGCATACCTGGGGCTGCTGGAGAAT GTGAGGGTCCGCAGGGCTGGCTTCGCTTCCCGCCAGCCCTACTCTCGATTCCTGCTCAGGTACAAGATGA CCTGTGAATACACATGGCCCAACCACCTGCTGGGCTCCGACAAGGCAGCCGTGAGCGCTCTCCTGGAGCA GCATGGGCTGCAGGGGGACGTGGCCTTTGGCCACAGCAAGCTGTTCATCCGCTCACCCCGGACACTGGTC ACACTGGAGCAGAGCCGAGCCCGCCTCATCCCCATCATTGTGCTGCTATTGCAGAAGGCATGGCGGGGCA CCTTGGCGAGGTGGCGCTGCCGGAGGCTGAGGGCTATCTACACCATCATGCGCTGGTTCCGGAGACACAA GGTGCGGGCTCACCTGGCTGAGCTGCAGCGGCGATTCCAGGCTGCAAGGCAGCCGCCACTCTACGGGCGT GACCTTGTGTGGCCGCTGCCCCCTGCTGTGCTGCAGCCCTTCCAGGACACCTGCCACGCACTCTTCTGCA GGTGGCGGGCCCGGCAGCTGGTGAAGAACATCCCCCCTTCAGACATGCCCCAGATCAAGGCCAAGGTGGC CGCCATGGGGGCCCTGCAAGGGCTTCGTCAGGACTGGGGCTGCCGACGGGCCTGGGCCCGAGACTACCTG TCCTCTGCCACTGACAATCCCACAGCATCAAGCCTGTTTGCTCAGCGACTAAAGACACTTCGGGACAAAG ATGGCTTCGGGGCTGTGCTCTTTTCAAGCCATGTCCGCAAGGTGAACCGCTTCCACAAGATCCGGAACCG GGCCCTCCTGCTCACAGACCAGCACCTCTACAAGCTGGACCCTGACCGGCAGTACCGGGTGATGCGGGCC GTGCCCCTTGAGGCGGTGACGGGGCTGAGCGTGACCAGCGGAGGAGACCAGCTGGTGGTGCTGCACGCCC GCGGCCAGGACGACCTCGTGGTGTGCCTGCACCGCTCCCGGCCGCCATTGGACAACCGCGTTGGGGAGCT GGTGGGCGTGCTGGCCGCACACTGCCAGGGGGAGGGCCGCACCCTGGAGGTTCGCGTCTCCGACTGCATC CCACTAAGCCATCGCGGGGTCCGGCGCCTCATCTCCGTGGAGCCCAGGCCGGAGCAGCCAGAGCCCGATT TCCGCTGCGCTCGCGGCTCCTTCACCCTGCTCTGGCCCAGCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218428 protein sequence
Red=Cloning site Green=Tags(s) MEDEEGPEYGKPDFVLLDQVTMEDFMRNLQLRFEKGRIYTYIGEVLVSVNPYQELPLYGPEAIARYQGRE LYERPPHLYAVANAAYKAMKHRSRDTCIVISGESGAGKTEASKHIMQYIAAVTNPSQRAEVERVKDVLLK STCVLEAFGNARTNRNHNSSRFGKYMDINFDFKGDPIGGHIHSYLLEKSRVLKQHVGERNFHAFYQLLRG SEDKQLHELHLERNPAVYNFTHQGAGLNMTVHSALDSDEQSHQAVTEAMRVIGFSPEEVESVHRILAAIL HLGNIEFVETEEGGLQKEGLAVAEEALVDHVAELTATPRDLVLRSLLARTVASGGRELIEKGHTAAEASY ARDACAKAVYQRLFEWVVNRINSVMEPRGRDPRRDGKDTVIGVLDIYGFEVFPVNSFEQFCINYCNEKLQ QLFIQLILKQEQEEYEREGITWQSVEYFNNATIVDLVERPHRGILAVLDEACSSAGTITDRIFLQTLDTH HRHHLHYTSRQLCPTDKTMEFGRDFRIKHYAGDVTYSVEGFIDKNRDFLFQDFKRLLYNSTDPTLRAMWP DGQQDITEVTKRPLTAGTLFKNSMVALVENLASKEPFYVRCIKPNEDKVAGKLDENHCRHQVAYLGLLEN VRVRRAGFASRQPYSRFLLRYKMTCEYTWPNHLLGSDKAAVSALLEQHGLQGDVAFGHSKLFIRSPRTLV TLEQSRARLIPIIVLLLQKAWRGTLARWRCRRLRAIYTIMRWFRRHKVRAHLAELQRRFQAARQPPLYGR DLVWPLPPAVLQPFQDTCHALFCRWRARQLVKNIPPSDMPQIKAKVAAMGALQGLRQDWGCRRAWARDYL SSATDNPTASSLFAQRLKTLRDKDGFGAVLFSSHVRKVNRFHKIRNRALLLTDQHLYKLDPDRQYRVMRA VPLEAVTGLSVTSGGDQLVVLHARGQDDLVVCLHRSRPPLDNRVGELVGVLAAHCQGEGRTLEVRVSDCI PLSHRGVRRLISVEPRPEQPEPDFRCARGSFTLLWPSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033054 |
| ORF Size | 3054 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033054.3 |
| RefSeq Size | 3313 bp |
| RefSeq ORF | 3057 bp |
| Locus ID | 64005 |
| UniProt ID | B0I1T2 |
| MW | 116.4 kDa |
| Gene Summary | MYO1G is a plasma membrane-associated class I myosin (see MIM 601478) that is abundant in T and B lymphocytes and mast cells (Pierce et al., 2001 [PubMed 11544309]; Patino-Lopez et al., 2010 [PubMed 20071333]).[supplied by OMIM, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218428L3 | Lenti ORF clone of Human myosin IG (MYO1G), Myc-DDK-tagged |
CNY 12,070.00 |
|
| RC218428L4 | Lenti ORF clone of Human myosin IG (MYO1G), mGFP tagged |
CNY 12,070.00 |
|
| RG218428 | MYO1G (tGFP-tagged) - Human myosin IG (MYO1G) |
CNY 11,210.00 |
|
| SC305543 | MYO1G (untagged)-Human myosin IG (MYO1G) |
CNY 7,096.00 |
其它Myosin 1G产品
