GPRC6A (NM_148963) Human Tagged ORF Clone

CAT＃: RC218656

GPRC6A (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 6, member A (GPRC6A)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_148963" in other vectors (6)

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CNY 6,784.00

CNY 9,790.00

货期*
现货
规格
    • 10 ug
Cited in 4 publications.

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA368435
GPRC6A rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

添加到购物车

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms bA86F4.3; GPCR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218656 representing NM_148963
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTCTTAATTATACTAATTACCTGCTTTGTGATTATTCTTGCTACTTCACAGCCTTGCCAGACCC
CTGATGACTTTGTGGCTGCCACTTCTCCGGGACATATCATAATTGGAGGTTTGTTTGCTATTCATGAAAA
AATGTTGTCCTCAGAAGACTCTCCCAGACGACCACAAATCCAGGAGTGTGTTGGCTTTGAAATATCAGTT
TTTCTTCAAACTCTTGCCATGATACACAGCATTGAGATGATCAACAATTCAACACTCTTATCTGGAGTCA
AACTGGGGTATGAAATCTATGACACTTGTACAGAAGTCACAGTGGCAATGGCAGCCACTCTGAGGTTTCT
TTCTAAATTCAACTGCTCCAGAGAAACTGTGGAGTTTAAGTGTGACTATTCCAGCTACATGCCAAGAGTT
AAGGCTGTCATAGGTTCTGGGTACTCAGAAATAACTATGGCTGTCTCCAGGATGTTGAATTTACAGCTCA
TGCCACAGGTGGGTTATGAATCAACTGCAGAAATCCTGAGTGACAAAATTCGCTTTCCTTCATTTTTACG
GACTGTGCCCAGTGACTTCCATCAAATTAAAGCAATGGCTCACCTGATTCAGAAATCTGGTTGGAACTGG
ATTGGCATCATAACCACAGATGATGACTATGGACGATTGGCTCTTAACACTTTTATAATTCAGGCTGAAG
CAAATAACGTGTGCATAGCCTTCAAAGAGGTTCTTCCAGCCTTTCTTTCAGATAATACCATTGAAGTCAG
AATCAATCGGACACTGAAGAAAATCATTTTAGAAGCCCAGGTTAATGTCATTGTGGTATTTCTGAGGCAA
TTCCATGTTTTTGATCTCTTCAATAAAGCCATTGAAATGAATATAAATAAGATGTGGATTGCTAGTGATA
ATTGGTCAACTGCCACCAAGATTACCACCATTCCTAATGTTAAAAAGATTGGCAAAGTTGTAGGGTTTGC
CTTTAGAAGAGGGAATATATCCTCTTTCCATTCCTTTCTTCAAAATCTGCACTTGCTTCCCAGTGACAGT
CACAAACTCTTACATGAATATGCCATGCATTTATCTGCCTGCGCATATGTCAAGGACACTGATTTGAGTC
AATGCATATTCAATCATTCTCAAAGGACTTTGGCCTACAAGGCTAACAAGGCTATAGAAAGGAACTTCGT
CATGAGAAATGACTTCCTCTGGGACTATGCTGAGCCAGGACTCATTCATAGTATTCAGCTTGCAGTGTTT
GCCCTTGGTTATGCCATTCGGGATCTGTGTCAAGCTCGTGACTGTCAGAACCCCAACGCCTTTCAACCAT
GGGAGTTACTTGGTGTGCTAAAAAATGTGACATTCACTGATGGATGGAATTCATTTCATTTTGATGCTCA
TGGGGATTTAAATACTGGATATGATGTTGTGCTCTGGAAGGAGATCAATGGACACATGACTGTCACTAAG
ATGGCAGAATATGACCTACAGAATGATGTCTTCATCATCCCAGATCAGGAAACAAAAAATGAGTTCAGGA
ATCTTAAGCAAATTCAATCTAAATGCTCCAAGGAATGCAGTCCTGGGCAAATGAAGAAAACTACAAGAAG
TCAACACATCTGTTGCTATGAATGTCAGAACTGTCCTGAAAATCATTACACTAATCAGACAGATATGCCT
CATTGCCTTTTATGCAACAACAAAACTCACTGGGCCCCTGTTAGGAGCACTATGTGCTTTGAAAAGGAAG
TGGAATATCTCAACTGGAATGACTCCTTGGCCATCCTACTCCTGATTCTCTCCCTACTGGGAATCATATT
TGTTCTGGTTGTTGGCATAATATTTACAAGAAACCTGAACACTCCTGTTGTGAAATCATCCGGGGGATTA
AGAGTCTGCTATGTGATCCTTCTCTGTCATTTCCTCAATTTTGCCAGCACGAGCTTTTTCATTGGAGAAC
CACAAGACTTCACATGTAAAACCAGGCAGACAATGTTTGGAGTGAGCTTTACTCTTTGCATCTCCTGCAT
TTTGACGAAGTCTCTGAAAATTTTGCTAGCTTTCAGCTTTGATCCCAAATTACAGAAATTTCTGAAGTGC
CTCTATAGACCGATCCTTATTATCTTCACTTGCACGGGCATCCAGGTTGTCATTTGCACACTCTGGCTAA
TCTTTGCAGCACCTACTGTAGAGGTGAATGTCTCCTTGCCCAGAGTCATCATCCTGGAGTGTGAGGAGGG
ATCCATACTTGCATTTGGCACCATGCTGGGCTACATTGCCATCCTGGCCTTCATTTGCTTCATATTTGCT
TTCAAAGGCAAATATGAGAATTACAATGAAGCCAAATTCATTACATTTGGCATGCTCATTTACTTCATAG
CTTGGATCACATTCATCCCTATCTATGCTACCACATTTGGCAAATATGTACCGGCTGTGGAGATTATTGT
CATATTAATATCTAACTATGGAATCCTGTATTGCACATTCATCCCCAAATGCTATGTTATTATTTGTAAG
CAAGAGATTAACACAAAGTCTGCCTTTCTCAAGATGATCTACAGTTATTCTTCCCATAGTGTGAGCAGCA
TTGCCCTGAGTCCTGCTTCACTGGACTCCATGAGCGGCAATGTCACAATGACCAATCCCAGCTCTAGTGG
CAAGTCTGCAACCTGGCAGAAAAGCAAAGATCTTCAGGCACAAGCATTTGCACACATATGCAGGGAAAAT
GCCACAAGTGTATCTAAAACTTTGCCTCGAAAAAGAATGTCAAGTATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC218656 representing NM_148963
Red=Cloning site Green=Tags(s)

MAFLIILITCFVIILATSQPCQTPDDFVAATSPGHIIIGGLFAIHEKMLSSEDSPRRPQIQECVGFEISV
FLQTLAMIHSIEMINNSTLLSGVKLGYEIYDTCTEVTVAMAATLRFLSKFNCSRETVEFKCDYSSYMPRV
KAVIGSGYSEITMAVSRMLNLQLMPQVGYESTAEILSDKIRFPSFLRTVPSDFHQIKAMAHLIQKSGWNW
IGIITTDDDYGRLALNTFIIQAEANNVCIAFKEVLPAFLSDNTIEVRINRTLKKIILEAQVNVIVVFLRQ
FHVFDLFNKAIEMNINKMWIASDNWSTATKITTIPNVKKIGKVVGFAFRRGNISSFHSFLQNLHLLPSDS
HKLLHEYAMHLSACAYVKDTDLSQCIFNHSQRTLAYKANKAIERNFVMRNDFLWDYAEPGLIHSIQLAVF
ALGYAIRDLCQARDCQNPNAFQPWELLGVLKNVTFTDGWNSFHFDAHGDLNTGYDVVLWKEINGHMTVTK
MAEYDLQNDVFIIPDQETKNEFRNLKQIQSKCSKECSPGQMKKTTRSQHICCYECQNCPENHYTNQTDMP
HCLLCNNKTHWAPVRSTMCFEKEVEYLNWNDSLAILLLILSLLGIIFVLVVGIIFTRNLNTPVVKSSGGL
RVCYVILLCHFLNFASTSFFIGEPQDFTCKTRQTMFGVSFTLCISCILTKSLKILLAFSFDPKLQKFLKC
LYRPILIIFTCTGIQVVICTLWLIFAAPTVEVNVSLPRVIILECEEGSILAFGTMLGYIAILAFICFIFA
FKGKYENYNEAKFITFGMLIYFIAWITFIPIYATTFGKYVPAVEIIVILISNYGILYCTFIPKCYVIICK
QEINTKSAFLKMIYSYSSHSVSSIALSPASLDSMSGNVTMTNPSSSGKSATWQKSKDLQAQAFAHICREN
ATSVSKTLPRKRMSSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_148963
ORF Size 2778 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_148963.4
RefSeq Size 2860 bp
RefSeq ORF 2781 bp
Locus ID 222545
UniProt ID Q5T6X5
Protein Families Druggable Genome, Transmembrane
MW 104.6 kDa
Gene Summary Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

The use of this cDNA Clones has been cited in the following citations:
SHBG141-161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and β-Langerhans cell lines ,De Toni, L;Guidolin, D;De Filippis, V;Peterle, D;Rocca, MS;Di Nisio, A;De Rocco Ponce, M;Foresta, C;, Sci Rep ,PubMed ID 31857654 [GPRC6A]
Membrane androgen receptor characteristics of human ZIP9 (SLC39A) zinc transporter in prostate cancer cells: Androgen-specific activation and involvement of an inhibitory G protein in zinc and MAP kinase signaling ,Thomas, P;Pang, Y;Dong, J;, Mol. Cell. Endocrinol ,PubMed ID 28219737 [GPRC6A]
Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A ,De Toni, L;Guidolin, D;De Filippis, V;Tescari, S;Strapazzon, G;Santa Rocca, M;Ferlin, A;Plebani, M;Foresta, C;, Endocrinology ,PubMed ID 27673554 [GPRC6A]
POLYMORPHISM rs2274911 OF GPRC6A AS A NOVEL RISK FACTOR FOR TESTIS FAILURE ,Toni, L;Nisio, AD;Speltra, E;Rocca, MS;Ghezzi, M;Zuccarello, D;Turiaco, N;Ferlin, A;Foresta, C;, J. Clin. Endocrinol. Metab. ,PubMed ID 26735260 [GPRC6A]

Other Versions

SKU Description Size Price
RC218656L1 Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), Myc-DDK-tagged
    • 10 ug

CNY 9,184.00

RC218656L2 Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), mGFP tagged
    • 10 ug

CNY 11,690.00

RC218656L3 Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), Myc-DDK-tagged
    • 10 ug

CNY 11,690.00

RC218656L4 Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), mGFP tagged
    • 10 ug

CNY 9,184.00

RG218656 GPRC6A (tGFP-tagged) - Human G protein-coupled receptor, family C, group 6, member A (GPRC6A)
    • 10 ug

CNY 8,384.00

SC306340 GPRC6A (untagged)-Human G protein-coupled receptor, family C, group 6, member A (GPRC6A)
    • 10 ug

CNY 6,792.00

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