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RNF190 (MARCH10) (NM_001100875) Human Tagged ORF Clone
CAT＃: RC218712
MARCH10 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2
ORF Plasmid: tGFP
"NM_001100875" in other vectors (4)
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CNY 4,896.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MARCH-X; MARCH10; RNF190 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218712 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCATA AGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAACG CGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAACAG AGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGTGACT CCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGGAAAGC AGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGACCAAGCACCAATGGTTTTATTAAGAAAGAGGAAA CCAAACCTGAGGAGATTTACAGTCAGCCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGAAGCAGAC AGAAACAGCAGTGGCCTGCAAAGGTGCCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAAGAAGGCCT GATGTGTAACACTAAGCTGAAGAGGCCAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCACAGCCAATG ACTGAGAACGCCCCTGATAGAGCCAAAAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAGCTGCATCCAG CCCTGTCCCAGGCCTTCCAAGAAAAAAATAGTCCTCAAGTATTGAGTGAGTTCTCGGGGCCACCACTCAC ACCCACCACTGTAGGAGGGCCAAGAAAGGCATCATTTAGGTTCCGAGATGAAGACTTTTATTCCATTTTG TCATTGAACAGCAGAAGAGAAAGCGATGACACTGAAGAGGAAACCCAGTCTGAAGAATGTCTGTGGGTTG GTGTGCGCTCACCCTGTTCTCCTTCCCATCACAAAAGAAGTAGATTTGGGGGGACATCGACCCCTCAGGC CAAAAATAAAAATTTTGAAGAAAATGCTGAAAATTGCAGAGGTCATTCTTCAAGAAGAAGTGAGCCCAGT CATGGCTCATTGAGAATAAGCAATGCAATGGAGCCAGCAACAGAGCGGCCTTCTGCTGGGCAAAGGTTGT CCCAAGACCCCGGGCTGCCTGATAGGGAATCTGCTACAGAGAAGGACAGAGGTGGCAGTGAAAATGCGAA AAAGAGCCCTCTTTCGTGGGACACCAAATCCGAGCCCAGACAAGAGGTTGGTGTCAATGCTGAAAATGTA TGGAGTGATTGTATTTCTGTGGAACATAGGCCTGGCACCCATGATTCTGAAGGATACTGGAAAGACTATT TAAACAGTTCTCAAAATTCTCTTGACTACTTTATTTCTGGCAGACCAATATCTCCAAGATCATCAGTGAA TTCATCCTATAACCCTCCTGCATCATTCATGCATTCTGCTCTGAGAGATGATATTCCAGTAGACTTGTCA ATGTCATCGACTTCAGTTCACAGCTCAGACTCAGAGGGAAACTCAGGGTTTCATGTTTGCCAACCACTGT CTCCCATTAGAAATAGGACTCCATTTGCCAGTGCCGAAAACCATAATTATTTCCCAGTAAACAGTGCACA CGAATTTGCTGTCAGGGAAGCAGAAGATACTACTTTAACAAGCCAGCCTCAGGGGGCTCCACTATATACA GATCTCTTACTAAATCCACAGGGCAATTTGTCCTTAGTGGATTCTTCCAGCTCCTCTCCATCAAGAATGA ACTCAGAAGGCCATTTGCATGTGTCTGGGTCTCTGCAAGAAAATACACCATTTACTTTCTTTGCAGTGTC TCATTTCCCAAATCAAAATGATAATGGGAGCAGGATGGCAGCCTCTGGTTTCACAGATGAAAAGGAAACC AGTAAGATAAAGGCAGACCCTGAGAAACTCAAGAAATTGCAAGAAAGTCTCCTGGAGGAGGACTCCGAGG AGGAGGGAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218712 protein sequence
Red=Cloning site Green=Tags(s) MLHDARDRQKFFSDVQYLRDMQHKVDSEYQACLRRQEYRRDPNEKKRDQFWGQETSFERSRFSSRSSSKQ SSSEEDALTEPRSSIKISAFKCDSKLPAIDQTSVKQKHKSTMTVRKAEKVDPSEPSPADQAPMVLLRKRK PNLRRFTVSPESHSPRASGDRSRQKQQWPAKVPVPRGADQVVQQEGLMCNTKLKRPNQERRNLVPSSQPM TENAPDRAKKGDPSAPSQSELHPALSQAFQEKNSPQVLSEFSGPPLTPTTVGGPRKASFRFRDEDFYSIL SLNSRRESDDTEEETQSEECLWVGVRSPCSPSHHKRSRFGGTSTPQAKNKNFEENAENCRGHSSRRSEPS HGSLRISNAMEPATERPSAGQRLSQDPGLPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVGVNAENV WSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPASFMHSALRDDIPVDLS MSSTSVHSSDSEGNSGFHVCQPLSPIRNRTPFASAENHNYFPVNSAHEFAVREAEDTTLTSQPQGAPLYT DLLLNPQGNLSLVDSSSSSPSRMNSEGHLHVSGSLQENTPFTFFAVSHFPNQNDNGSRMAASGFTDEKET SKIKADPEKLKKLQESLLEEDSEEEGD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100875 |
| ORF Size | 1971 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100875.3 |
| RefSeq Size | 3072 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 162333 |
| UniProt ID | Q8NA82 |
| MW | 73.5 kDa |
| Gene Summary | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218712L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218712L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG218712 | 41343 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2 |
CNY 4,370.00 |
|
| SC316836 | 41343 (untagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2 |
CNY 7,220.00 |
