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Prol1 (OPRPN) (NM_021225) Human Tagged ORF Clone
CAT＃: RC218759
- TrueORF®
PROL1 (Myc-DDK-tagged)-Human proline rich, lacrimal 1 (PROL1)
ORF Plasmid: tGFP
"NM_021225" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BPLP; opiorphin; PRL1; PROL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218759 representing NM_021225
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATTAACTTTCTTCTTGGGCCTGTTGGCTCTTATTTCATGTTTCACACCCAGTGAGAGTCAAAGAT TCTCCAGAAGACCATATCTACCTGGCCAGCTGCCACCACCTCCACTCTACAGGCCAAGATGGGTTCCACC AAGTCCCCCACCTCCCTATGACTCAAGACTTAATTCACCACTTTCTCTTCCCTTTGTCCCAGGGCGAGTT CCACCATCTTCTTTCTCTCGATTTAGCCAAGCAGTCATTCTATCTCAACTCTTTCCATTGGAATCTATTA GACAACCTCGACTCTTTCCGGGTTATCCAAACCTACATTTCCCACTAAGACCTTACTATGTAGGACCTAT TAGGATATTAAAACCCCCATTTCCTCCTATTCCTTTTTTTCTTGCTATTTACCTTCCTATCTCTAACCCT GAGCCCCAAATAAACATCACCACCGCAGATACAACAATCACCACAAATCCCCCCACCACTGCAACAGCAA CCACCAGCACTTCCACAAAACCCACAATGACGATCAGCTCCTCAACAGTACCTATCTCTTCAACACCAGA GCCTGCCACCTCCATATCAGCAGCAACCCCCGCAGCATCTACTGAAAATACTACTCAAATTCTCGCCAAC CGTCCTCACACAGTATTGCTCAATGCCACTGTCCAAGTTACGACTTCCAACCAAACTATATTAAGCAGCC CAGCCTTTAAAAGTTTTTGGCAAAAACTCTTTGCCATTTTTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218759 representing NM_021225
Red=Cloning site Green=Tags(s) MKLTFFLGLLALISCFTPSESQRFSRRPYLPGQLPPPPLYRPRWVPPSPPPPYDSRLNSPLSLPFVPGRV PPSSFSRFSQAVILSQLFPLESIRQPRLFPGYPNLHFPLRPYYVGPIRILKPPFPPIPFFLAIYLPISNP EPQINITTADTTITTNPPTTATATTSTSTKPTMTISSSTVPISSTPEPATSISAATPAASTENTTQILAN RPHTVLLNATVQVTTSNQTILSSPAFKSFWQKLFAIFG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021225 |
| ORF Size | 744 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021225.5 |
| RefSeq Size | 1060 bp |
| RefSeq ORF | 747 bp |
| Locus ID | 58503 |
| UniProt ID | Q99935 |
| Protein Families | Secreted Protein |
| MW | 27 kDa |
| Gene Summary | This gene encodes a member of the proline-rich protein family. The encoded protein has multiple proposed functions, including roles in pain suppression, penile erection, and protection of the eye surface. The QRFSR pentapeptide, known as opiorphin, is derived from the N-terminal of this protein. Opiorphin inhibits the enkephalin-inactivating peptidases neprilysin and aminopeptidase N, and this activity is thought to reduce sensitivity to painful stimuli by effecting enkephalin-related activation of opioid-dependent pathways. Opiorphin may also act as an anti-depressant. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218759L1 | Lenti ORF clone of Human proline rich, lacrimal 1 (PROL1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC218759L2 | Lenti ORF clone of Human proline rich, lacrimal 1 (PROL1), mGFP tagged |
CNY 5,890.00 |
|
| RC218759L3 | Lenti ORF clone of Human proline rich, lacrimal 1 (PROL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218759L4 | Lenti ORF clone of Human proline rich, lacrimal 1 (PROL1), mGFP tagged |
CNY 4,800.00 |
|
| RG218759 | PROL1 (tGFP-tagged) - Human proline rich, lacrimal 1 (PROL1) |
CNY 4,000.00 |
|
| SC304924 | PROL1 (untagged)-Human proline rich, lacrimal 1 (PROL1) |
CNY 2,400.00 |
