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MTGR1 (CBFA2T2) (NM_001032999) Human Tagged ORF Clone
CAT＃: RC218940
- TrueORF®
CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 3
ORF Plasmid: tGFP
"NM_001032999" in other vectors (4)
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CNY 4,432.00
CNY 5,230.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EHT; MTGR1; p85; ZMYND3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218940 representing NM_001032999
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGGCGTCCCTGGAGCGGCCGCCTTCCAGCTTGGTCCTGAGAAAAGGGTGCCAGCGATGCCTGGAT CGCCTGTGGAAGTGAAGATACAGTCCAGATCCTCACCTCCCACCATGCCACCCCTCCCACCAATAAATCC TGGAGGACCGAGGCCAGTGTCCTTCACTCCTACTGCATTAAGCAATGGCATCAACCATTCTCCTCCTACC CTGAATGGTGCCCCATCACCGCCACAGAGATTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCA CAAATCAGCAATTGCCAGCCACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCT GCAACAGTTTGGCAATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGTG AACTCAACAGTGACAATTGAGGAATTCCACTGTAAGCTCCAAGAAGCCACAAACTTTCCCCTTCGTCCTT TTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACTGCTGCACTGCGCTCGGGCGGC CAAGCAGACCCCATCCCAGTACCTGGCTCAGCACGAACACCTTCTGCTCAACACAAGCATTGCATCGCCT GCTGACTCGTCAGAGTTGCTCATGGAGGTGCACGGAAATGGGAAGAGGCCCAGTCCAGAGAGGAGAGAAG AGAATAGTTTTGATAGAGACACAATTGCTCCTGAGCCTCCTGCCAAGAGAGTATGTACCATCAGCCCTGC TCCTCGGCACAGTCCTGCTCTCACTGTGCCCCTCATGAATCCCGGGGGCCAATTCCATCCTACCCCTCCA CCTCTTCAGCATTACACCTTAGAGGATATTGCAACTTCTCACCTGTATCGGGAACCCAACAAGATGCTAG AGCATCGAGAAGTTCGTGATAGACACCACAGTCTTGGTCTAAATGGAGGCTATCAAGATGAGTTGGTAGA TCATCGTTTGACAGAAAGGGAATGGGCTGATGAATGGAAACATCTTGACCATGCGCTGAATTGCATTATG GAAATGGTAGAGAAAACAAGGCGCTCTATGGCAGTTCTGCGGCGCTGTCAGGAATCAGATCGTGAAGAAC TCAACTACTGGAAAAGACGGTACAATGAAAACACAGAGCTGAGGAAAACGGGGACCGAGTTGGTCTCCAG GCAGCACAGCCCTGGGAGTGCAGATTCTCTCAGCAATGATTCTCAGAGAGAGTTCAACAGCAGGCCAGGT ACAGGATACGTACCTGTGGAGTTTTGGAAAAAAACAGAAGAAGCTGTGAATAAGGTGAAAATTCAGGCCA TGTCAGAAGTACAGAAGGCCGTCGCTGAGGCAGAGCAGAAAGCCTTTGAAGTGATTGCAACAGAGAGAGC ACGAATGGAGCAAACCATAGCGGATGTCAAGCGGCAGGCCGCAGAGGATGCTTTCCTCGTCATCAATGAG CAAGAGGAGTCCACGGAGAACTGCTGGAACTGTGGCCGCAAAGCCAGCGAGACATGCAGTGGCTGCAATA TCGCGCGATACTGTGGCTCTTTCTGCCAGCACAAGGACTGGGAGCGGCACCACCGCCTCTGTGGTCAGAA CCTGCATGGCCAGAGCCCCCACGGCCAGGGCCGGCCGCTGCTTCCTGTAGGCAGGGGCTCCTCTGCCAGG TCCGCCGACTGCAGCGTGCCCAGCCCAGCCCTCGACAAGACCTCGGCAACCACATCGCGTTCCTCAACAC CTGCTTCTGTGACAGCTATCGACACCAACGGACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218940 representing NM_001032999
Red=Cloning site Green=Tags(s) MVGVPGAAAFQLGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPRPVSFTPTALSNGINHSPPT LNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALV NSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHLLLNTSIASP ADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHSPALTVPLMNPGGQFHPTPP PLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLTEREWADEWKHLDHALNCIM EMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSPGSADSLSNDSQREFNSRPG TGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAAEDAFLVINE QEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQSPHGQGRPLLPVGRGSSAR SADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001032999 |
| ORF Size | 1785 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001032999.3 |
| RefSeq Size | 6517 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 9139 |
| UniProt ID | O43439 |
| Protein Families | Transcription Factors |
| MW | 65.7 kDa |
| Gene Summary | In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218940L3 | Lenti-ORF clone of CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 3 |
CNY 7,130.00 |
|
| RC218940L4 | Lenti-ORF clone of CBFA2T2 (mGFP-tagged)-Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 3 |
CNY 7,130.00 |
|
| RG218940 | CBFA2T2 (tGFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 3 |
CNY 5,700.00 |
|
| SC302668 | CBFA2T2 (untagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 3 |
CNY 9,600.00 |
