EPX (NM_000502) Human Tagged ORF Clone
CAT#: RC218959
- TrueORF®
EPX (Myc-DDK-tagged)-Human eosinophil peroxidase (EPX)
ORF Plasmid: tGFP
"NM_000502" in other vectors (4)
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CNY 5,240.00
CNY 5,800.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EPO; EPP; EPX-PEN; EPXD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218959 representing NM_000502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCTGCTCCCAGCCCTGGCAGGGGTCCTGGCCACACTCGTCCTCGCCCAGCCCTGTGAGGGCACTG ACCCAGCCTCCCCTGGGGCAGTGGAGACCTCGGTCCTGCGAGACTGCATAGCAGAGGCCAAGTTGCTGGT GGATGCTGCCTACAATTGGACCCAGAAGAGCATCAAGCAGCGGCTTCGCAGCGGTTCAGCCAGCCCCATG GACCTCCTGTCCTACTTCAAACAACCGGTAGCAGCCACCAGGACAGTTGTTCGGGCCGCAGATTATATGC ATGTGGCTTTGGGGCTGCTTGAAGAGAAGTTACAACCCCAGCGGTCCGGACCCTTCAATGTCACTGATGT GCTAACAGAACCACAGCTGCGGCTGCTGTCCCAGGCCAGTGGCTGTGCTCTCCGGGACCAGGCCGAGCGC TGCAGCGACAAGTACCGCACCATCACTGGACGGTGCAACAACAAGAGGAGACCCTTGCTAGGGGCCTCCA ACCAGGCTCTGGCTCGCTGGCTGCCCGCCGAGTATGAGGATGGGCTGTCGCTCCCCTTCGGCTGGACCCC CAGCAGGAGGCGCAATGGCTTCCTTCTCCCTCTTGTCCGGGCTGTCTCCAACCAGATTGTGCGCTTCCCC AATGAGAGACTGACCTCCGACCGTGGCCGAGCCCTCATGTTCATGCAGTGGGGCCAGTTCATTGACCATG ACCTGGACTTCTCCCCGGAGTCCCCGGCCAGAGTGGCCTTCACTGCAGGCGTTGACTGTGAGAGGACCTG CGCCCAGCTGCCCCCCTGCTTTCCCATCAAGATCCCACCCAATGACCCCCGCATCAAGAACCAGCGTGAC TGCATCCCTTTCTTCCGCTCGGCACCCTCATGCCCCCAAAACAAGAACAGAGTCCGCAACCAGATCAACG CGCTCACCTCCTTTGTGGACGCCAGCATGGTGTATGGCAGTGAGGTCTCCCTCTCGCTGCGGCTCCGCAA CCGGACCAACTACCTGGGGCTGCTGGCCATCAACCAGCGCTTTCAAGACAACGGCCGGGCCCTGCTGCCC TTCGACAACCTGCACGATGACCCCTGTCTCCTCACCAACCGCTCGGCGCGCATCCCCTGCTTCCTGGCAG GTGACACCCGATCAACGGAAACCCCCAAACTGGCAGCCATGCACACCCTCTTTATGCGAGAGCACAACCG GCTGGCCACCGAGCTGAGACGCCTGAATCCCCGGTGGAATGGAGACAAACTGTACAATGAGGCTCGGAAG ATCATGGGGGCCATGGTCCAGATCATCACCTACCGAGACTTTCTGCCCCTGGTTCTGGGCAAGGCCCGGG CCAGGAGAACCCTGGGGCACTACAGGGGGTACTGCTCCAATGTGGACCCACGGGTGGCCAATGTCTTCAC CCTGGCCTTCCGCTTTGGCCACACAATGCTCCAGCCCTTCATGTTCCGCTTGGACAGTCAGTACCGGGCC TCCGCACCCAACTCGCATGTCCCACTTAGCTCTGCCTTCTTTGCCAGCTGGCGGATCGTGTATGAAGGGG GCATCGACCCCATCCTCCGGGGCCTCATGGCCACCCCTGCCAAGCTGAACCGTCAGGATGCCATGTTAGT GGATGAGCTCCGGGACCGGCTGTTTCGGCAAGTGAGGAGGATTGGGCTGGACCTGGCAGCTCTCAACATG CAACGAAGCCGGGACCACGGCCTTCCAGGGTACAATGCTTGGAGGCGCTTCTGTGGGCTCTCCCAGCCCC GGAATTTGGCACAGCTTAGCCGGGTGCTGAAAAACCAGGACTTGGCAAGGAAGTTCCTGAATTTGTATGG AACACCTGACAACATTGACATCTGGATTGGGGCCATCGCTGAGCCTCTTTTGCCGGGGGCTCGAGTGGGG CCTCTTCTGGCTTGTCTGTTCGAGAACCAGTTCAGAAGAGCCCGAGACGGAGACAGGTTCTGGTGGCAGA AACGAGGTGTTTTCACCAAAAGACAGCGCAAGGCCCTGAGCAGAATTTCCTTGTCTCGAATTATATGTGA CAATACCGGTATCACCACGGTTTCAAGGGACATCTTCAGAGCCAACATCTACCCTCGGGGCTTTGTGAAC TGCAGCCGTATCCCCAGGTTGAACCTATCAGCCTGGCGAGGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218959 representing NM_000502
Red=Cloning site Green=Tags(s) MHLLPALAGVLATLVLAQPCEGTDPASPGAVETSVLRDCIAEAKLLVDAAYNWTQKSIKQRLRSGSASPM DLLSYFKQPVAATRTVVRAADYMHVALGLLEEKLQPQRSGPFNVTDVLTEPQLRLLSQASGCALRDQAER CSDKYRTITGRCNNKRRPLLGASNQALARWLPAEYEDGLSLPFGWTPSRRRNGFLLPLVRAVSNQIVRFP NERLTSDRGRALMFMQWGQFIDHDLDFSPESPARVAFTAGVDCERTCAQLPPCFPIKIPPNDPRIKNQRD CIPFFRSAPSCPQNKNRVRNQINALTSFVDASMVYGSEVSLSLRLRNRTNYLGLLAINQRFQDNGRALLP FDNLHDDPCLLTNRSARIPCFLAGDTRSTETPKLAAMHTLFMREHNRLATELRRLNPRWNGDKLYNEARK IMGAMVQIITYRDFLPLVLGKARARRTLGHYRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYRA SAPNSHVPLSSAFFASWRIVYEGGIDPILRGLMATPAKLNRQDAMLVDELRDRLFRQVRRIGLDLAALNM QRSRDHGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLNLYGTPDNIDIWIGAIAEPLLPGARVG PLLACLFENQFRRARDGDRFWWQKRGVFTKRQRKALSRISLSRIICDNTGITTVSRDIFRANIYPRGFVN CSRIPRLNLSAWRGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000502 |
ORF Size | 2145 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000502.6 |
RefSeq Size | 2731 bp |
RefSeq ORF | 2148 bp |
Locus ID | 8288 |
UniProt ID | P11678 |
Domains | An_peroxidase |
Protein Families | Druggable Genome |
Protein Pathways | Asthma |
MW | 81.5 kDa |
Gene Summary | This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Feb 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia
,Nagel, S;Pommerenke, C;Meyer, C;MacLeod, RAF;Drexler, HG;,
PLoS One
,PubMed ID 33048949
[EPX]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218959L3 | Lenti-ORF clone of EPX (Myc-DDK-tagged)-Human eosinophil peroxidase (EPX) |
CNY 7,700.00 |
|
RC218959L4 | Lenti-ORF clone of EPX (mGFP-tagged)-Human eosinophil peroxidase (EPX) |
CNY 7,700.00 |
|
RG218959 | EPX (tGFP-tagged) - Human eosinophil peroxidase (EPX) |
CNY 6,840.00 |
|
SC119861 | EPX (untagged)-Human eosinophil peroxidase (EPX) |
CNY 5,248.00 |