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TNNI3K (NM_015978) Human Tagged ORF Clone
CAT＃: RC219069
TNNI3K (Myc-DDK-tagged)-Human TNNI3 interacting kinase (TNNI3K)
ORF Plasmid: tGFP
"NM_015978" in other vectors (6)
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CNY 6,120.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CARK; CCDD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219069 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATTATAAATCTAGACCAACCCAAACTTGTACTGATGAATGGAAGAAAAAAGTCAGTGAATCAT ATGTTATCACAATAGAAAGATTAGAAGATGACCTGCAGATCAAGGAAAAAGAACTGACAGAACTAAGGAA TATATTTGGCTCTGATGAAGCCTTCAGTAAAGTCAATTTAAATTACCGCACTGAAAATGGGCTGTCTCTA CTTCATTTATGTTGCATTTGTGGAGGCAAGAAATCACATATTCGAACTCTTATGTTGAAAGGGCTCCGCC CATCTCGACTGACAAGAAATGGATTTACAGCCTTGCATTTAGCAGTTTACAAGGATAATGCAGAATTGAT CACTTCTCTGCTTCACAGTGGAGCTGATATACAGCAGGTTGGATACGGTGGCCTCACTGCCCTCCATATT GCTACAATAGCTGGCCACCTAGAGGCTGCTGATGTGCTGTTGCAACATGGAGCTAATGTCAATATTCAAG ATGCAGTTTTTTTCACTCCATTGCATATTGCAGCGTACTATGGACATGAACAGGTAACTCGCCTTCTTTT GAAATTTGGTGCTGATGTAAATGTAAGTGGTGAAGTTGGAGATAGACCCCTCCACCTAGCATCTGCAAAA GGATTCTTGAATATTGCAAAACTCTTGATGGAAGAAGGCAGCAAAGCAGATGTGAATGCTCAAGATAATG AAGACCATGTCCCACTCCATTTCTGTTCTCGATTTGGACACCATGATATAGTTAAGTATCTGCTGCAAAG TGATTTGGAAGTTCAACCTCATGTTGTTAATATCTATGGAGATACCCCCTTACACCTGGCATGCTACAAT GGCAAATTTGAAGTTGCCAAGGAAATCATCCAAATATCAGGAACAGAAAGTCTGACTAAGGAAAACATCT TCAGTGAAACAGCTTTTCATAGTGCTTGTACCTATGGCAAGAGCATTGACCTAGTCAAATTTCTTCTTGA TCAGAATGTCATAAACATCAACCACCAAGGAAGGGATGGGCACACTGGATTACACTCTGCTTGCTACCAC GGTCACATTCGCCTGGTTCAGTTCTTACTGGATAATGGAGCTGATATGAATCTAGTGGCTTGTGATCCCA GCAGGTCTAGTGGTGAAAAAGATGAGCAGACATGTTTGATGTGGGCTTATGAAAAAGGGCATGATGCCAT TGTCACACTCCTGAAGCATTATAAGAGACCACAAGATGAATTGCCCTGTAATGAATATTCTCAGCCTGGA GGAGATGGCTCCTATGTGTCTGTTCCATCACCCTTGGGGAAGATTAAAAGCATGACAAAAGAGAAGGCAG ATATTCTCCTCCTAAGAGCTGGATTGCCTTCACATTTCCATCTTCAGCTCTCAGAAATTGAGTTCCATGA GATTATTGGCTCAGGTTCTTTTGGGAAAGTATATAAAGGACGATGCAGAAATAAAATAGTGGCTATAAAA CGTTATCGAGCCAATACCTACTGCTCCAAGTCAGATGTGGATATGTTTTGCCGAGAGGTGTCCATTCTCT GCCAGCTCAATCATCCCTGCGTAATTCAGTTTGTGGGTGCTTGCTTGAATGATCCCAGCCAGTTTGCCAT TGTCACTCAATACATATCAGGGGGTTCTCTGTTCTCCCTCCTTCATGAGCAGAAGAGGATTCTTGATTTG CAGTCTAAATTAATTATTGCAGTAGATGTTGCCAAAGGCATGGAGTACCTTCACAACCTGACACAGCCAA TTATACATCGTGACTTGAACAGTCACAATATTCTTCTCTATGAGGATGGGCATGCTGTGGTGGCAGATTT TGGAGAATCAAGATTTCTACAGTCTCTGGATGAAGACAACATGACAAAACAACCTGGGAACCTCCGTTGG ATGGCTCCTGAGGTGTTCACGCAGTGCACTCGGTACACCATCAAAGCAGATGTCTTCAGCTATGCTCTGT GTCTGTGGGAAATTCTCACTGGCGAAATTCCATTCGCTCATCTCAAGCCAGCGGCTGCGGCAGCAGACAT GGCTTACCACCACATCAGACCTCCCATTGGCTATTCCATTCCCAAGCCCATATCATCTCTGCTGATACGA GGGTGGAACGCATGTCCTGAAGGAAGACCCGAATTTTCTGAAGTTGTCATGAAGTTAGAAGAGTGTCTCT GCAACATTGAGCTGATGTCTCCTGCATCAAGTAACAGCAGTGGGTCTCTCTCACCTTCTTCTTCTTCTGA TTGCCTGGTGAACCGGGGAGGACCTGGCCGGAGTCATGTGGCAGCATTAAGAAGTCGTTTCGAATTGGAA TATGCTCTAAATGCAAGGTCCTATGCTGCTTTGTCCCAAAGTGCTGGACAATATTCCTCTCAAGGTCTGT CTTTGGAGGAGATGAAAAGAAGTCTTCAATACACACCCATTGACAAATATGGCTATGTATCCGATCCCAT GAGCTCAATGCATTTTCATTCTTGCCGAAATAGTAGCAGCTTTGAGGACAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219069 protein sequence
Red=Cloning site Green=Tags(s) MGNYKSRPTQTCTDEWKKKVSESYVITIERLEDDLQIKEKELTELRNIFGSDEAFSKVNLNYRTENGLSL LHLCCICGGKKSHIRTLMLKGLRPSRLTRNGFTALHLAVYKDNAELITSLLHSGADIQQVGYGGLTALHI ATIAGHLEAADVLLQHGANVNIQDAVFFTPLHIAAYYGHEQVTRLLLKFGADVNVSGEVGDRPLHLASAK GFLNIAKLLMEEGSKADVNAQDNEDHVPLHFCSRFGHHDIVKYLLQSDLEVQPHVVNIYGDTPLHLACYN GKFEVAKEIIQISGTESLTKENIFSETAFHSACTYGKSIDLVKFLLDQNVININHQGRDGHTGLHSACYH GHIRLVQFLLDNGADMNLVACDPSRSSGEKDEQTCLMWAYEKGHDAIVTLLKHYKRPQDELPCNEYSQPG GDGSYVSVPSPLGKIKSMTKEKADILLLRAGLPSHFHLQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIK RYRANTYCSKSDVDMFCREVSILCQLNHPCVIQFVGACLNDPSQFAIVTQYISGGSLFSLLHEQKRILDL QSKLIIAVDVAKGMEYLHNLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRW MAPEVFTQCTRYTIKADVFSYALCLWEILTGEIPFAHLKPAAAAADMAYHHIRPPIGYSIPKPISSLLIR GWNACPEGRPEFSEVVMKLEECLCNIELMSPASSNSSGSLSPSSSSDCLVNRGGPGRSHVAALRSRFELE YALNARSYAALSQSAGQYSSQGLSLEEMKRSLQYTPIDKYGYVSDPMSSMHFHSCRNSSSFEDSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015978 |
| ORF Size | 2505 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015978.3 |
| RefSeq Size | 3026 bp |
| RefSeq ORF | 2508 bp |
| Locus ID | 51086 |
| UniProt ID | Q59H18 |
| Domains | pkinase, TyrKc, ANK, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 92.9 kDa |
| Gene Summary | This gene encodes a protein that belongs to the MAP kinase kinase kinase (MAPKKK) family of protein kinases. The protein contains ankyrin repeat, protein kinase and serine-rich domains and is thought to play a role in cardiac physiology. [provided by RefSeq, Sep 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219069L1 | Lenti ORF clone of Human TNNI3 interacting kinase (TNNI3K), Myc-DDK-tagged |
CNY 8,520.00 |
|
| RC219069L2 | Lenti ORF clone of Human TNNI3 interacting kinase (TNNI3K), mGFP tagged |
CNY 9,030.00 |
|
| RC219069L3 | Lenti ORF clone of Human TNNI3 interacting kinase (TNNI3K), Myc-DDK-tagged |
CNY 8,520.00 |
|
| RC219069L4 | Lenti ORF clone of Human TNNI3 interacting kinase (TNNI3K), mGFP tagged |
CNY 9,030.00 |
|
| RG219069 | TNNI3K (tGFP-tagged) - Human TNNI3 interacting kinase (TNNI3K) |
CNY 7,720.00 |
|
| SC314794 | TNNI3K (untagged)-Human TNNI3 interacting kinase (TNNI3K) |
CNY 7,220.00 |
